Phylogenetic and systematic studies of the amorphophallus species in Sarawak

A systematic study was conducted on ten species of Amorphophallus in Sarawak based on morphology, ecology and genomic sequences data. The objectives of this study were; (I) to document morphological characteristics and ecological data of ten selected Amorphophallus species in Sarawak; (2) to find a...

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Main Author:	Aizat, Japar
Format:	Thesis
Language:	English
Published:	2013
Subjects:	SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/14257/1/Aizat%20Japar%20ft.pdf
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id	my-unimas-ir.14257
record_format	uketd_dc
spelling	my-unimas-ir.142572023-03-28T03:13:36Z Phylogenetic and systematic studies of the amorphophallus species in Sarawak 2013 Aizat, Japar SB Plant culture A systematic study was conducted on ten species of Amorphophallus in Sarawak based on morphology, ecology and genomic sequences data. The objectives of this study were; (I) to document morphological characteristics and ecological data of ten selected Amorphophallus species in Sarawak; (2) to find a suitable method of DNA extraction (3) to reconstruct phylogenetic tree and examine the phylogenetic relationship between the Amorphophallus speciej) Materials to be used as herbarium specimen and DNA samples were collected, from localities around Sarawak such as Kuching, Sri Aman, Padawan and Mulu. Random amplified polymorphic DNA technique was applied and genetic profiles were obtained for the selected species generated using primers OPC-06, OPD-05 and OPD-20. Dendrogram created using the unweighted pair group method with arithmetic averages provide enough information for the analysis of genetic diversity. Phylogenetic trees for Amorphophallus species were reconstructed using two genomic sequence, rbcL genes and trnL-F intergenic spacer. The sequenced data was analyzed by four different methods; Bayesian, maximum likelihood, maximum parsimony and neighbor joining. The out-groups for the phylogenetic trees are from genus Arisaema, DiefJenbachia, and Bognera. The reconstructed phylogenetic trees were successful to resolve phylogenetic relationship for several species ofAmorphophallus. Universiti Malaysia Sarawak (UNIMAS) 2013 Thesis http://ir.unimas.my/id/eprint/14257/ http://ir.unimas.my/id/eprint/14257/1/Aizat%20Japar%20ft.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Aizat, Japar Phylogenetic and systematic studies of the amorphophallus species in Sarawak
description	A systematic study was conducted on ten species of Amorphophallus in Sarawak based on morphology, ecology and genomic sequences data. The objectives of this study were; (I) to document morphological characteristics and ecological data of ten selected Amorphophallus species in Sarawak; (2) to find a suitable method of DNA extraction (3) to reconstruct phylogenetic tree and examine the phylogenetic relationship between the Amorphophallus speciej) Materials to be used as herbarium specimen and DNA samples were collected, from localities around Sarawak such as Kuching, Sri Aman, Padawan and Mulu. Random amplified polymorphic DNA technique was applied and genetic profiles were obtained for the selected species generated using primers OPC-06, OPD-05 and OPD-20. Dendrogram created using the unweighted pair group method with arithmetic averages provide enough information for the analysis of genetic diversity. Phylogenetic trees for Amorphophallus species were reconstructed using two genomic sequence, rbcL genes and trnL-F intergenic spacer. The sequenced data was analyzed by four different methods; Bayesian, maximum likelihood, maximum parsimony and neighbor joining. The out-groups for the phylogenetic trees are from genus Arisaema, DiefJenbachia, and Bognera. The reconstructed phylogenetic trees were successful to resolve phylogenetic relationship for several species ofAmorphophallus.
format	Thesis
qualification_level	Master's degree
author	Aizat, Japar
author_facet	Aizat, Japar
author_sort	Aizat, Japar
title	Phylogenetic and systematic studies of the amorphophallus species in Sarawak
title_short	Phylogenetic and systematic studies of the amorphophallus species in Sarawak
title_full	Phylogenetic and systematic studies of the amorphophallus species in Sarawak
title_fullStr	Phylogenetic and systematic studies of the amorphophallus species in Sarawak
title_full_unstemmed	Phylogenetic and systematic studies of the amorphophallus species in Sarawak
title_sort	phylogenetic and systematic studies of the amorphophallus species in sarawak
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2013
url	http://ir.unimas.my/id/eprint/14257/1/Aizat%20Japar%20ft.pdf
_version_	1783728155504148480

Phylogenetic and systematic studies of the amorphophallus species in Sarawak

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