Ecology and systematics studies of three amorphophallus species from Borneo

Three selected Amorphophallus species that were A. borneensis (Engl. ) Engl. & Gehrm. from Kalimantan, A. hewittii Alderw. from Sarawak and A. lambii Mayo & Widjaja. from Sabah of Borneo were studied to determine the genetic relatedness based on both morphological and DNA fingerprinting. Th...

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Main Author:	Angeline, Simon
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QK Botany SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/14861/1/Angeline%20Simon%20ft.pdf
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id	my-unimas-ir.14861
record_format	uketd_dc
spelling	my-unimas-ir.148612023-03-29T03:02:17Z Ecology and systematics studies of three amorphophallus species from Borneo 2010 Angeline, Simon QK Botany SB Plant culture Three selected Amorphophallus species that were A. borneensis (Engl. ) Engl. & Gehrm. from Kalimantan, A. hewittii Alderw. from Sarawak and A. lambii Mayo & Widjaja. from Sabah of Borneo were studied to determine the genetic relatedness based on both morphological and DNA fingerprinting. The three species studied have closed resemblance in their morphology characteristics and therefore two molecular markers were selected for the DNA fingerprinting which were the Direct Amplified Minisatellite Region (DAMD) using M 13 universal primer and the Random Amplified Polymorphic DNA (RAPD) using two OPD primers which were OPD 2 and OPD 2& The detail morphology characters for the three species were easily distinguished for the following characters which were the length of the peduncle, the spathe opening, base surface of inner spathe and the shape of stigma. However there were variation in the patterns and colours of both the leaf petiole and peduncle of the inflorescence within the species collected from different localities. Relationship between all of the species studied were determined by the Unweighted Pair-Group Method with Arithmetical Averages (UPGMA) analysed using the Ntsys-pc Numerical Taxonomy and Multivariate Analysis System Version 2.2 Exeter Software. Based on the data obtained three dendograms were generated. Both PCR-DAMD using M13 universal primer and PCR-RAPD using OPD 20 primer showed clear clustering into three groups separating each species according to the clusters. However PCR-RAPD using OPD 2 primer was able to differentiate the species but showed different relatedness compared to M13 universal primer and OPD 20 primer. Using the neighbour joining analysis, two dendograms were generated. The dendogram generated based on the PCR-DAMD using M13 universal primer and based on the PCR-RAPD using both OPD 2 and OPD 20 primer showed different relatedness among all three species studied. Universiti Malaysia Sarawak, (UNIMAS) 2010 Thesis http://ir.unimas.my/id/eprint/14861/ http://ir.unimas.my/id/eprint/14861/1/Angeline%20Simon%20ft.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QK Botany SB Plant culture
spellingShingle	QK Botany SB Plant culture Angeline, Simon Ecology and systematics studies of three amorphophallus species from Borneo
description	Three selected Amorphophallus species that were A. borneensis (Engl. ) Engl. & Gehrm. from Kalimantan, A. hewittii Alderw. from Sarawak and A. lambii Mayo & Widjaja. from Sabah of Borneo were studied to determine the genetic relatedness based on both morphological and DNA fingerprinting. The three species studied have closed resemblance in their morphology characteristics and therefore two molecular markers were selected for the DNA fingerprinting which were the Direct Amplified Minisatellite Region (DAMD) using M 13 universal primer and the Random Amplified Polymorphic DNA (RAPD) using two OPD primers which were OPD 2 and OPD 2& The detail morphology characters for the three species were easily distinguished for the following characters which were the length of the peduncle, the spathe opening, base surface of inner spathe and the shape of stigma. However there were variation in the patterns and colours of both the leaf petiole and peduncle of the inflorescence within the species collected from different localities. Relationship between all of the species studied were determined by the Unweighted Pair-Group Method with Arithmetical Averages (UPGMA) analysed using the Ntsys-pc Numerical Taxonomy and Multivariate Analysis System Version 2.2 Exeter Software. Based on the data obtained three dendograms were generated. Both PCR-DAMD using M13 universal primer and PCR-RAPD using OPD 20 primer showed clear clustering into three groups separating each species according to the clusters. However PCR-RAPD using OPD 2 primer was able to differentiate the species but showed different relatedness compared to M13 universal primer and OPD 20 primer. Using the neighbour joining analysis, two dendograms were generated. The dendogram generated based on the PCR-DAMD using M13 universal primer and based on the PCR-RAPD using both OPD 2 and OPD 20 primer showed different relatedness among all three species studied.
format	Thesis
qualification_level	Master's degree
author	Angeline, Simon
author_facet	Angeline, Simon
author_sort	Angeline, Simon
title	Ecology and systematics studies of three amorphophallus species from Borneo
title_short	Ecology and systematics studies of three amorphophallus species from Borneo
title_full	Ecology and systematics studies of three amorphophallus species from Borneo
title_fullStr	Ecology and systematics studies of three amorphophallus species from Borneo
title_full_unstemmed	Ecology and systematics studies of three amorphophallus species from Borneo
title_sort	ecology and systematics studies of three amorphophallus species from borneo
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2010
url	http://ir.unimas.my/id/eprint/14861/1/Angeline%20Simon%20ft.pdf
_version_	1783728171274731520

Ecology and systematics studies of three amorphophallus species from Borneo

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