Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)

Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)

Hand Foot and Mouth Disease is a common exanthema of young children, characterized by fever, rash on the palm and bottoms of the feet and ulcers in the oral cavity. In this study, the entire region of CV A16 genome for 22 CV A16 strains associated with HFMD in Sarawak (Malaysia Borneo), isolated be...

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Main Author: Katrina, binti Aslan Joe
Format: Thesis
Language:English
Published: 2012
Subjects:
RJ Pediatrics
Online Access:http://ir.unimas.my/id/eprint/15886/3/Katrina%20ft.pdf
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spelling my-unimas-ir.158862024-08-20T06:50:03Z Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007) 2012 Katrina, binti Aslan Joe RJ Pediatrics Hand Foot and Mouth Disease is a common exanthema of young children, characterized by fever, rash on the palm and bottoms of the feet and ulcers in the oral cavity. In this study, the entire region of CV A16 genome for 22 CV A16 strains associated with HFMD in Sarawak (Malaysia Borneo), isolated between 1997 and 2008 were successfully sequenced. There were 25 sets of full CVA16 genome primers which were designed in such a way that it targets the whole genome of CVA16 regions in addition to three anti-sense gene specific primers that were used to amplify the 5'UTR region and two sense gene-specific primers that were used to amplify the 3'UTR region of the CVA16 genome. These primers successfully amplified the expected product size from extracted RNA of all 22 isolates. Universiti Malaysia Sarawak, (UNIMAS) 2012 Thesis http://ir.unimas.my/id/eprint/15886/ http://ir.unimas.my/id/eprint/15886/3/Katrina%20ft.pdf text en validuser masters Universiti Malaysia Sarawak, (UNIMAS) Institutes of Health and Community Medicine
institution Universiti Malaysia Sarawak
collection UNIMAS Institutional Repository
language English
topic RJ Pediatrics
spellingShingle RJ Pediatrics
Katrina, binti Aslan Joe
Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)
description Hand Foot and Mouth Disease is a common exanthema of young children, characterized by fever, rash on the palm and bottoms of the feet and ulcers in the oral cavity. In this study, the entire region of CV A16 genome for 22 CV A16 strains associated with HFMD in Sarawak (Malaysia Borneo), isolated between 1997 and 2008 were successfully sequenced. There were 25 sets of full CVA16 genome primers which were designed in such a way that it targets the whole genome of CVA16 regions in addition to three anti-sense gene specific primers that were used to amplify the 5'UTR region and two sense gene-specific primers that were used to amplify the 3'UTR region of the CVA16 genome. These primers successfully amplified the expected product size from extracted RNA of all 22 isolates.
format Thesis
qualification_level Master's degree
author Katrina, binti Aslan Joe
author_facet Katrina, binti Aslan Joe
author_sort Katrina, binti Aslan Joe
title Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)
title_short Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)
title_full Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)
title_fullStr Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)
title_full_unstemmed Complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in Sarawak (1999-2007)
title_sort complete genome characterization of non-hev71 species a enteroviruses associated with hand, foot and mouth disease in sarawak (1999-2007)
granting_institution Universiti Malaysia Sarawak, (UNIMAS)
granting_department Institutes of Health and Community Medicine
publishDate 2012
url http://ir.unimas.my/id/eprint/15886/3/Katrina%20ft.pdf
_version_ 1811771540521353216

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