Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation

Eleven Total Petroleum Hydrocarbons (TPH)-degrading bacteria were successfully isolated from six inocula obtained from Seria Crude Oil Terminal (SCOT) plant in Brunei Darussalam using selective enrichment technique. All isolates were cultivated in liquid media with crude oil as the sole carbon and...

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Main Author:	Pearlycia ., Brooke
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/18916/1/Pearlycia%20Brooke%20ft.pdf
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id	my-unimas-ir.18916
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spelling	my-unimas-ir.189162023-06-22T05:32:47Z Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation 2010 Pearlycia ., Brooke QR Microbiology Eleven Total Petroleum Hydrocarbons (TPH)-degrading bacteria were successfully isolated from six inocula obtained from Seria Crude Oil Terminal (SCOT) plant in Brunei Darussalam using selective enrichment technique. All isolates were cultivated in liquid media with crude oil as the sole carbon and energy source. The nucleotide sequences of the 16S rRNA gene of these bacteria were determined. Based on biochemical and molecilar characterization performed, the identities of the bacteria capable of degrading hydrocarbons belong to the genus Bacillus, Brevibacillus, Pseudomonas, Agrobacterioum and Stenotrophomonas. All these isolates were assessed for their resistance against antibiotocs like gentamicin, cephalexin, tetracycline, penicillin, erythromycin and vancomycin by mean of Kirby-Bauer disk diffusion method. Universiti Malaysia Sarawak (UNIMAS) 2010 Thesis http://ir.unimas.my/id/eprint/18916/ http://ir.unimas.my/id/eprint/18916/1/Pearlycia%20Brooke%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology.
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Pearlycia ., Brooke Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation
description	Eleven Total Petroleum Hydrocarbons (TPH)-degrading bacteria were successfully isolated from six inocula obtained from Seria Crude Oil Terminal (SCOT) plant in Brunei Darussalam using selective enrichment technique. All isolates were cultivated in liquid media with crude oil as the sole carbon and energy source. The nucleotide sequences of the 16S rRNA gene of these bacteria were determined. Based on biochemical and molecilar characterization performed, the identities of the bacteria capable of degrading hydrocarbons belong to the genus Bacillus, Brevibacillus, Pseudomonas, Agrobacterioum and Stenotrophomonas. All these isolates were assessed for their resistance against antibiotocs like gentamicin, cephalexin, tetracycline, penicillin, erythromycin and vancomycin by mean of Kirby-Bauer disk diffusion method.
format	Thesis
qualification_level	Master's degree
author	Pearlycia ., Brooke
author_facet	Pearlycia ., Brooke
author_sort	Pearlycia ., Brooke
title	Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation
title_short	Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation
title_full	Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation
title_fullStr	Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation
title_full_unstemmed	Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation
title_sort	identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology.
publishDate	2010
url	http://ir.unimas.my/id/eprint/18916/1/Pearlycia%20Brooke%20ft.pdf
_version_	1783728202596745216

Identification and characterization of total petroleum hydrocarbon-degrading bacteria isolated from inoculum tanks and their potential in bioremediation

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