Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production

This study was performed to conduct genetic improvement of the oleaginous yeast Lipomyces starkeyi for the enhancement of lipid production. Strain improvement was carried out via random mutagenesis by using ethidium bromide treatment and mutant selection by using cerulenin. Ten mutant isolates demon...

Full description

Saved in:

Bibliographic Details
Main Author:	Latifah, Binti Suali
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Q Science (General) QH Natural history
Online Access:	http://ir.unimas.my/id/eprint/21153/1/Latifah.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimas-ir.21153
record_format	uketd_dc
spelling	my-unimas-ir.211532023-06-27T07:55:56Z Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production 2017 Latifah, Binti Suali Q Science (General) QH Natural history This study was performed to conduct genetic improvement of the oleaginous yeast Lipomyces starkeyi for the enhancement of lipid production. Strain improvement was carried out via random mutagenesis by using ethidium bromide treatment and mutant selection by using cerulenin. Ten mutant isolates demonstrating normal growth in the presence of cerulenin were considered as good candidates for further experiments. The selected mutant strains were designated as L. starkeyi MV-1, L. starkeyi MV-2, L. starkeyi MV-3, L. starkeyi MV-4, L. starkeyi MV-5, L. starkeyi MV-6, L. starkeyi MV-7, L. starkeyi MV-8, L. starkeyi MV-9 and L. starkeyi MV-10. The strains were studied for genetic variability using a M13-PCR fingerprinting approach with L. starkeyi ATCC 12659 as a reference strain. A total of 11 distinctive PCR patterns were obtained which consisted of 7 to 12 bands with the sizes ranging from 280 to 2000 bp. A dendogram was constructed by the unweighted-pair group average (UPGMA) using linkages available in the PyElph software version 1.3. unimas 2017 Thesis http://ir.unimas.my/id/eprint/21153/ http://ir.unimas.my/id/eprint/21153/1/Latifah.pdf text en validuser masters UNIMAS
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QH Natural history
spellingShingle	Q Science (General) QH Natural history Latifah, Binti Suali Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production
description	This study was performed to conduct genetic improvement of the oleaginous yeast Lipomyces starkeyi for the enhancement of lipid production. Strain improvement was carried out via random mutagenesis by using ethidium bromide treatment and mutant selection by using cerulenin. Ten mutant isolates demonstrating normal growth in the presence of cerulenin were considered as good candidates for further experiments. The selected mutant strains were designated as L. starkeyi MV-1, L. starkeyi MV-2, L. starkeyi MV-3, L. starkeyi MV-4, L. starkeyi MV-5, L. starkeyi MV-6, L. starkeyi MV-7, L. starkeyi MV-8, L. starkeyi MV-9 and L. starkeyi MV-10. The strains were studied for genetic variability using a M13-PCR fingerprinting approach with L. starkeyi ATCC 12659 as a reference strain. A total of 11 distinctive PCR patterns were obtained which consisted of 7 to 12 bands with the sizes ranging from 280 to 2000 bp. A dendogram was constructed by the unweighted-pair group average (UPGMA) using linkages available in the PyElph software version 1.3.
format	Thesis
qualification_level	Master's degree
author	Latifah, Binti Suali
author_facet	Latifah, Binti Suali
author_sort	Latifah, Binti Suali
title	Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production
title_short	Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production
title_full	Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production
title_fullStr	Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production
title_full_unstemmed	Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production
title_sort	strain improvement of lipomyces starkeyi atcc 12659 via random mutagenesis for optimized lipid production
granting_institution	UNIMAS
publishDate	2017
url	http://ir.unimas.my/id/eprint/21153/1/Latifah.pdf
_version_	1783728226638495744

Strain Improvement of Lipomyces starkeyi ATCC 12659 via Random Mutagenesis for Optimized Lipid Production

Similar Items