Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model

Fusarium oxyporum is a pathogenic fungus that is responsible for severe plant diseases on many economically important crops including Piper nigrum L. One of these, a destructive disease commonly known as Fusarium wilt, has led to an economic loss in Malaysian pepper industry. The molecular pathoge...

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Main Author:	Nurul Haniza, Binti Zaini
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Q Science (General) QH301 Biology
Online Access:	http://ir.unimas.my/id/eprint/21453/1/Nurul%20Haniza%20Zaini%20ft.pdf
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id	my-unimas-ir.21453
record_format	uketd_dc
spelling	my-unimas-ir.214532023-05-18T09:10:47Z Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model 2017 Nurul Haniza, Binti Zaini Q Science (General) QH301 Biology Fusarium oxyporum is a pathogenic fungus that is responsible for severe plant diseases on many economically important crops including Piper nigrum L. One of these, a destructive disease commonly known as Fusarium wilt, has led to an economic loss in Malaysian pepper industry. The molecular pathogenicity arsenal of the F. oxyporum in response to the infection was investigated. Current work presented the development of an artificial induction model for the study of the differentially expressed gene transcripts of F. oxyporum upon its interaction with Piper nigrum L. The assessment of the differentially expressed genes (DEGs) during pre induction and post-induction was done by Differential-Display Reverse-Transcriptase PCR (DDRT-PCR) by cDNA-RAPD approach, and the identified DEGs were sequenced. Based on the models tested: In-situ Induction Models and In-vitro Induction Models, the In-vitro Induction Model 6 (ivIM6) was established and selected as the most suitable artificial induction model due to its feasibility in pathogen visualization, effective sampling strategy and elimination of endophytic contamination. By utilizing the optimized four RAPD primers, a total of seven DEGs were identified and sequenced. Out of seven DEGs, three DEGs were up regulated and four DEGs were down-regulated by the employment of DDRT-PCR via cDNA RAPD approach. The bioinformatics analyses of the up-regulated transcripts were revealed to be involved in the F. oxyporum morphogenesis and pathogenicity mechanism. unimas 2017 Thesis http://ir.unimas.my/id/eprint/21453/ http://ir.unimas.my/id/eprint/21453/1/Nurul%20Haniza%20Zaini%20ft.pdf text en public masters UNIMAS
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QH301 Biology
spellingShingle	Q Science (General) QH301 Biology Nurul Haniza, Binti Zaini Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model
description	Fusarium oxyporum is a pathogenic fungus that is responsible for severe plant diseases on many economically important crops including Piper nigrum L. One of these, a destructive disease commonly known as Fusarium wilt, has led to an economic loss in Malaysian pepper industry. The molecular pathogenicity arsenal of the F. oxyporum in response to the infection was investigated. Current work presented the development of an artificial induction model for the study of the differentially expressed gene transcripts of F. oxyporum upon its interaction with Piper nigrum L. The assessment of the differentially expressed genes (DEGs) during pre induction and post-induction was done by Differential-Display Reverse-Transcriptase PCR (DDRT-PCR) by cDNA-RAPD approach, and the identified DEGs were sequenced. Based on the models tested: In-situ Induction Models and In-vitro Induction Models, the In-vitro Induction Model 6 (ivIM6) was established and selected as the most suitable artificial induction model due to its feasibility in pathogen visualization, effective sampling strategy and elimination of endophytic contamination. By utilizing the optimized four RAPD primers, a total of seven DEGs were identified and sequenced. Out of seven DEGs, three DEGs were up regulated and four DEGs were down-regulated by the employment of DDRT-PCR via cDNA RAPD approach. The bioinformatics analyses of the up-regulated transcripts were revealed to be involved in the F. oxyporum morphogenesis and pathogenicity mechanism.
format	Thesis
qualification_level	Master's degree
author	Nurul Haniza, Binti Zaini
author_facet	Nurul Haniza, Binti Zaini
author_sort	Nurul Haniza, Binti Zaini
title	Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model
title_short	Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model
title_full	Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model
title_fullStr	Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model
title_full_unstemmed	Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model
title_sort	differential gene expression study of fusarium oxyporum upon interaction with pepper (piper nigrum l.) via artificial induction model
granting_institution	UNIMAS
publishDate	2017
url	http://ir.unimas.my/id/eprint/21453/1/Nurul%20Haniza%20Zaini%20ft.pdf
_version_	1783728228674830336

Differential Gene Expression Study of Fusarium oxyporum Upon Interaction with Pepper (Piper nigrum L.) via Artificial Induction Model

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