Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties

Cancer is one of the most feared diseases in the world and is the second leading cause of ath worldwide. With the progression of modern technology, there is an upsurge in the use of natural resources as a direct medicine for cancer treatment. Combining the focus towards the use of natural resource f...

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Main Author:	Nurhazlina, Hamdan
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General) QH301 Biology
Online Access:	http://ir.unimas.my/id/eprint/25225/3/Nurhazlin.pdf
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id	my-unimas-ir.25225
record_format	uketd_dc
spelling	my-unimas-ir.252252023-05-22T07:00:22Z Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties 2019-05 Nurhazlina, Hamdan Q Science (General) QH301 Biology Cancer is one of the most feared diseases in the world and is the second leading cause of ath worldwide. With the progression of modern technology, there is an upsurge in the use of natural resources as a direct medicine for cancer treatment. Combining the focus towards the use of natural resource for cancer medicine and the developing interest on peptides as the anticancer agent has led to this research study. The aim of the study was to discover proteins possessing potential anticancer activity from locally used medicinal plants. Several common plants that traditionally known to have medicinal properties which are Orthosiphon aristatus, Gynura procumbens, Clinacanthus nutans, Piper sarmentosum, Piper betle and Senna alata were used as the source samples. The crude protein extracts for these plants were extracted overnight by using two buffers; Phosphate Buffered Saline (PBS) and HEPES buffer. Protein profiling and quantification were performed by using SDS-PAGE and Bradford assay respectively. Nasopharyngeal cell lines (NP-69; normal cell line and HK-1; cancer cell line) were used to test the crude protein extracts for anticancer activity, resultedin narrowing down the targeted plant to Orthosiphon aristatus and Piper sarmentosum. The two plants samples were fractionized using ammonium sulfate precipitation technique and the fractionized sample cell toxicity values were measured by using MTT assay. The active fractions from both plants sample were identified using LC-MS/MS spectrometry resulted on a list of identified proteins. Universiti Malaysia Sarawak (UNIMAS) 2019-05 Thesis http://ir.unimas.my/id/eprint/25225/ http://ir.unimas.my/id/eprint/25225/3/Nurhazlin.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QH301 Biology
spellingShingle	Q Science (General) QH301 Biology Nurhazlina, Hamdan Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties
description	Cancer is one of the most feared diseases in the world and is the second leading cause of ath worldwide. With the progression of modern technology, there is an upsurge in the use of natural resources as a direct medicine for cancer treatment. Combining the focus towards the use of natural resource for cancer medicine and the developing interest on peptides as the anticancer agent has led to this research study. The aim of the study was to discover proteins possessing potential anticancer activity from locally used medicinal plants. Several common plants that traditionally known to have medicinal properties which are Orthosiphon aristatus, Gynura procumbens, Clinacanthus nutans, Piper sarmentosum, Piper betle and Senna alata were used as the source samples. The crude protein extracts for these plants were extracted overnight by using two buffers; Phosphate Buffered Saline (PBS) and HEPES buffer. Protein profiling and quantification were performed by using SDS-PAGE and Bradford assay respectively. Nasopharyngeal cell lines (NP-69; normal cell line and HK-1; cancer cell line) were used to test the crude protein extracts for anticancer activity, resultedin narrowing down the targeted plant to Orthosiphon aristatus and Piper sarmentosum. The two plants samples were fractionized using ammonium sulfate precipitation technique and the fractionized sample cell toxicity values were measured by using MTT assay. The active fractions from both plants sample were identified using LC-MS/MS spectrometry resulted on a list of identified proteins.
format	Thesis
qualification_level	Master's degree
author	Nurhazlina, Hamdan
author_facet	Nurhazlina, Hamdan
author_sort	Nurhazlina, Hamdan
title	Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties
title_short	Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties
title_full	Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties
title_fullStr	Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties
title_full_unstemmed	Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties
title_sort	identification of proteins from selected medicinal plants with potential anticancer properties
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2019
url	http://ir.unimas.my/id/eprint/25225/3/Nurhazlin.pdf
_version_	1783728293508284416

Identification of Proteins from Selected Medicinal Plants with Potential Anticancer Properties

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