Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)
Metroxylon sagu can be found in two forms known as trunking and non-trunking sago palm. Trunking sago palm is the plant that contains high amount of starch accumulated in the pith of its trunk whereas non-trunking sago palm has a stunted growth of the trunk. Non-trunking sago palm can lead to...
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| 主要作者: | |
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| 格式: | Thesis |
| 语言: | English |
| 出版: |
2019
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| 主题: | |
| 在线阅读: | http://ir.unimas.my/id/eprint/25237/3/Rapid%20Amplification%20of%20cDNA%20Ends%20of%20Differentially%20Expressed%20Genes.pdf |
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| 总结: | Metroxylon sagu can be found in two forms known as trunking and non-trunking sago palm.
Trunking sago palm is the plant that contains high amount of starch accumulated in the pith
of its trunk whereas non-trunking sago palm has a stunted growth of the trunk. Non-trunking
sago palm can lead to wastage of various resources especially production of starch. The
failure in the formation of trunk for non-trunking sago palm might be related to specific
genes which are over or under expressed. Research which have been done previously on
differential expressed transcripts of trunking and non-trunking sago palm showed that there
were several partial gene transcripts successfully distinguished between trunking and non
trunking sago palm. Since the gene sequences that are related to the formation and the failure
to form a trunk of sago palm are still not fully determined, five of these transcripts were
selected in an attempt to obtain full length cDNA sequences. The selected candidate genes
in this study were DET1, DET2, DET3, DET4, and DET5. The 5’ and 3’ RACE were done
by using total RNA from young leaves of sago palm. The 3’ends for five candidate genes
were successfully obtained. For the 5’ends however, only DET1 gene sequence was
successfully amplified. Then, new sequences were constructed and analysed, and found that
each DET1, DET2, DET3, DET4, and DET5 are highly matched with peptidyl prolyl cis
trans isomerase, beta-glucosidase, dnaJ, S-adenosylmethionine synthase and ascorbate
peroxidase. Based on the additional sequence obtained in this study, this information can be
used in a developing database specifically for trunking and non-trunking sago palm and to
study further on the roles of these candidate genes in sago palm. |
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