Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)

Metroxylon sagu can be found in two forms known as trunking and non-trunking sago palm. Trunking sago palm is the plant that contains high amount of starch accumulated in the pith of its trunk whereas non-trunking sago palm has a stunted growth of the trunk. Non-trunking sago palm can lead to...

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Main Author:	Zulaikha, Binti Pol Ong
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General) QH301 Biology
Online Access:	http://ir.unimas.my/id/eprint/25237/3/Rapid%20Amplification%20of%20cDNA%20Ends%20of%20Differentially%20Expressed%20Genes.pdf
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spelling	my-unimas-ir.252372023-06-13T03:54:32Z Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.) 2019-05-27 Zulaikha, Binti Pol Ong Q Science (General) QH301 Biology Metroxylon sagu can be found in two forms known as trunking and non-trunking sago palm. Trunking sago palm is the plant that contains high amount of starch accumulated in the pith of its trunk whereas non-trunking sago palm has a stunted growth of the trunk. Non-trunking sago palm can lead to wastage of various resources especially production of starch. The failure in the formation of trunk for non-trunking sago palm might be related to specific genes which are over or under expressed. Research which have been done previously on differential expressed transcripts of trunking and non-trunking sago palm showed that there were several partial gene transcripts successfully distinguished between trunking and non trunking sago palm. Since the gene sequences that are related to the formation and the failure to form a trunk of sago palm are still not fully determined, five of these transcripts were selected in an attempt to obtain full length cDNA sequences. The selected candidate genes in this study were DET1, DET2, DET3, DET4, and DET5. The 5’ and 3’ RACE were done by using total RNA from young leaves of sago palm. The 3’ends for five candidate genes were successfully obtained. For the 5’ends however, only DET1 gene sequence was successfully amplified. Then, new sequences were constructed and analysed, and found that each DET1, DET2, DET3, DET4, and DET5 are highly matched with peptidyl prolyl cis trans isomerase, beta-glucosidase, dnaJ, S-adenosylmethionine synthase and ascorbate peroxidase. Based on the additional sequence obtained in this study, this information can be used in a developing database specifically for trunking and non-trunking sago palm and to study further on the roles of these candidate genes in sago palm. 2019-05 Thesis http://ir.unimas.my/id/eprint/25237/ http://ir.unimas.my/id/eprint/25237/3/Rapid%20Amplification%20of%20cDNA%20Ends%20of%20Differentially%20Expressed%20Genes.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Molecular Biology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QH301 Biology
spellingShingle	Q Science (General) QH301 Biology Zulaikha, Binti Pol Ong Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)
description	Metroxylon sagu can be found in two forms known as trunking and non-trunking sago palm. Trunking sago palm is the plant that contains high amount of starch accumulated in the pith of its trunk whereas non-trunking sago palm has a stunted growth of the trunk. Non-trunking sago palm can lead to wastage of various resources especially production of starch. The failure in the formation of trunk for non-trunking sago palm might be related to specific genes which are over or under expressed. Research which have been done previously on differential expressed transcripts of trunking and non-trunking sago palm showed that there were several partial gene transcripts successfully distinguished between trunking and non trunking sago palm. Since the gene sequences that are related to the formation and the failure to form a trunk of sago palm are still not fully determined, five of these transcripts were selected in an attempt to obtain full length cDNA sequences. The selected candidate genes in this study were DET1, DET2, DET3, DET4, and DET5. The 5’ and 3’ RACE were done by using total RNA from young leaves of sago palm. The 3’ends for five candidate genes were successfully obtained. For the 5’ends however, only DET1 gene sequence was successfully amplified. Then, new sequences were constructed and analysed, and found that each DET1, DET2, DET3, DET4, and DET5 are highly matched with peptidyl prolyl cis trans isomerase, beta-glucosidase, dnaJ, S-adenosylmethionine synthase and ascorbate peroxidase. Based on the additional sequence obtained in this study, this information can be used in a developing database specifically for trunking and non-trunking sago palm and to study further on the roles of these candidate genes in sago palm.
format	Thesis
qualification_level	Master's degree
author	Zulaikha, Binti Pol Ong
author_facet	Zulaikha, Binti Pol Ong
author_sort	Zulaikha, Binti Pol Ong
title	Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)
title_short	Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)
title_full	Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)
title_fullStr	Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)
title_full_unstemmed	Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)
title_sort	rapid amplification of cdna ends of differentially expressed genes from trunking and non-trunking sago palm (metroxylon sagu rottb.)
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Molecular Biology
publishDate	2019
url	http://ir.unimas.my/id/eprint/25237/3/Rapid%20Amplification%20of%20cDNA%20Ends%20of%20Differentially%20Expressed%20Genes.pdf
_version_	1783728294065078272

Rapid Amplification of cDNA Ends of Differentially Expressed Genes from Trunking and Non-Trunking Sago Palm (Metroxylon Sagu Rottb.)

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