Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma

Osteosarcoma is the most common primary malignancy of bone with worldwide incidence of 2 to 4 million/year. This tumor usually developed at distal part of fast growing bone and associated with instability of chromosome 6. Despite that, the preceding molecular mechanism leading to the instability is...

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Main Author:	Ramli, Mohd Rozi
Format:	Thesis
Language:	English
Published:	2019
Subjects:	RB Pathology
Online Access:	http://ir.unimas.my/id/eprint/25380/1/Mohd%20Rozi%20Ramli%20ft.pdf
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spelling	my-unimas-ir.253802023-06-27T09:14:02Z Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma 2019-06-24 Ramli, Mohd Rozi RB Pathology Osteosarcoma is the most common primary malignancy of bone with worldwide incidence of 2 to 4 million/year. This tumor usually developed at distal part of fast growing bone and associated with instability of chromosome 6. Despite that, the preceding molecular mechanism leading to the instability is not fully known. Following the local ethical committee review, a total of 31 cases within 12 years were collected to investigate the copy number of gene encoding Vascular Endothelial Growth Factor (VEGF) located at chromosome 6p21 using Fluorescence in situ Hybridization (FISH) and the general chromosomal instability as indicated by micronuclei formation. In addition to VEGF, Hypoxia inducible factor 1α (HIF-1α) and p53 diagnostic immunohistochemistry (IHC) staining were performed as to investigate relationship of hypoxic stress and tumor growth suppress. Furthermore, the Mitogen Activated Protein Kinase (MAPK) cellular proliferation pathway molecules; ERK, p38 and JNK IHC were also examined. Findings showed VEGF expression at both protein and gene level (6p21). On top of that, expressed ERK, p38 but not JNK; and expressed HIF & p53 in those cases suggest tumor cell survival by evading the natural cell death program, apoptosis. In conclusion, this study presented a possible molecular mechanism underlying chromosomal instability in human osteosarcoma. Universiti Malaysia Sarawak (UNIMAS) 2019-06 Thesis http://ir.unimas.my/id/eprint/25380/ http://ir.unimas.my/id/eprint/25380/1/Mohd%20Rozi%20Ramli%20ft.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of medicine and health sciences
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	RB Pathology
spellingShingle	RB Pathology Ramli, Mohd Rozi Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma
description	Osteosarcoma is the most common primary malignancy of bone with worldwide incidence of 2 to 4 million/year. This tumor usually developed at distal part of fast growing bone and associated with instability of chromosome 6. Despite that, the preceding molecular mechanism leading to the instability is not fully known. Following the local ethical committee review, a total of 31 cases within 12 years were collected to investigate the copy number of gene encoding Vascular Endothelial Growth Factor (VEGF) located at chromosome 6p21 using Fluorescence in situ Hybridization (FISH) and the general chromosomal instability as indicated by micronuclei formation. In addition to VEGF, Hypoxia inducible factor 1α (HIF-1α) and p53 diagnostic immunohistochemistry (IHC) staining were performed as to investigate relationship of hypoxic stress and tumor growth suppress. Furthermore, the Mitogen Activated Protein Kinase (MAPK) cellular proliferation pathway molecules; ERK, p38 and JNK IHC were also examined. Findings showed VEGF expression at both protein and gene level (6p21). On top of that, expressed ERK, p38 but not JNK; and expressed HIF & p53 in those cases suggest tumor cell survival by evading the natural cell death program, apoptosis. In conclusion, this study presented a possible molecular mechanism underlying chromosomal instability in human osteosarcoma.
format	Thesis
qualification_level	Master's degree
author	Ramli, Mohd Rozi
author_facet	Ramli, Mohd Rozi
author_sort	Ramli, Mohd Rozi
title	Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma
title_short	Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma
title_full	Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma
title_fullStr	Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma
title_full_unstemmed	Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma
title_sort	elucidating the underlying molecular pathways mediating chromosomal instability in human osteosarcoma
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of medicine and health sciences
publishDate	2019
url	http://ir.unimas.my/id/eprint/25380/1/Mohd%20Rozi%20Ramli%20ft.pdf
_version_	1783728296897282048

Elucidating the Underlying Molecular Pathways Mediating Chromosomal Instability in Human Osteosarcoma

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