Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi

Phenanthrene is a type of polycyclic aromatic hydrocarbon (PAH). It is an organic pollutant that exists persistently in the environment, resulting mainly from anthropogenic activities. Out of the 24 different fungi that had been successfully isolated from water hyacinth (Eicchornia crassipes), 5 fun...

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Main Author:	Dalene, Lesen
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/26239/1/Dalene%20anak%20Lesen%20ft.pdf
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id	my-unimas-ir.26239
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spelling	my-unimas-ir.262392023-05-11T02:52:44Z Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi 2019 Dalene, Lesen Q Science (General) QR Microbiology Phenanthrene is a type of polycyclic aromatic hydrocarbon (PAH). It is an organic pollutant that exists persistently in the environment, resulting mainly from anthropogenic activities. Out of the 24 different fungi that had been successfully isolated from water hyacinth (Eicchornia crassipes), 5 fungal isolates showed the ability to degrade phenanthrene. Isolates EC08 and EC22, showed higher rate of phenanthrene degradation as compared to isolates EC04, EC13, and EC18. Percentage of phenanthrene degradation of EC08 and EC22 after 7 days was shown to be 8.26% and 14.19%, respectively. All five isolates showed the presence of manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase (Lacc) activities in the presence of phenanthrene. The magnitude of the enzyme activities did not correlate with the percentage of phenanthrene degradation by the fungi. This suggests that other enzymes apart from the ligninolytic enzymes might be involved in the degradation pathways, and that the phenanthrene degradation involves a complex multienzymatic system. The physical and chemical parameters that can affect the growth of fungi and the rate of phenanthrene degradation were studied. It was observed that, each of the isolate showed different preferences in the pH of the growth media, the supplemental carbon, as well as the supplemental nitrogen. Identification of the five isolates was done based on their morphology as well as through molecular analysis. Through the analysis, isolates EC04, EC08, and EC22 were identified as Phlebia acanthocystis, Penicillium pinophilum, and Aspergillus versicolor respectively, while isolates EC13 and EC18 were identified as Inonotus pachyphyleous T61 and Inonotus pachyphloeus RS1, respectively. Keywords: Phenanthrene, degradation, ligninolytic enzymes, manganese peroxidase, lignin peroxidase, laccase Universiti Malaysia Sarawak (UNIMAS) 2019 Thesis http://ir.unimas.my/id/eprint/26239/ http://ir.unimas.my/id/eprint/26239/1/Dalene%20anak%20Lesen%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Dalene, Lesen Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi
description	Phenanthrene is a type of polycyclic aromatic hydrocarbon (PAH). It is an organic pollutant that exists persistently in the environment, resulting mainly from anthropogenic activities. Out of the 24 different fungi that had been successfully isolated from water hyacinth (Eicchornia crassipes), 5 fungal isolates showed the ability to degrade phenanthrene. Isolates EC08 and EC22, showed higher rate of phenanthrene degradation as compared to isolates EC04, EC13, and EC18. Percentage of phenanthrene degradation of EC08 and EC22 after 7 days was shown to be 8.26% and 14.19%, respectively. All five isolates showed the presence of manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase (Lacc) activities in the presence of phenanthrene. The magnitude of the enzyme activities did not correlate with the percentage of phenanthrene degradation by the fungi. This suggests that other enzymes apart from the ligninolytic enzymes might be involved in the degradation pathways, and that the phenanthrene degradation involves a complex multienzymatic system. The physical and chemical parameters that can affect the growth of fungi and the rate of phenanthrene degradation were studied. It was observed that, each of the isolate showed different preferences in the pH of the growth media, the supplemental carbon, as well as the supplemental nitrogen. Identification of the five isolates was done based on their morphology as well as through molecular analysis. Through the analysis, isolates EC04, EC08, and EC22 were identified as Phlebia acanthocystis, Penicillium pinophilum, and Aspergillus versicolor respectively, while isolates EC13 and EC18 were identified as Inonotus pachyphyleous T61 and Inonotus pachyphloeus RS1, respectively. Keywords: Phenanthrene, degradation, ligninolytic enzymes, manganese peroxidase, lignin peroxidase, laccase
format	Thesis
qualification_level	Master's degree
author	Dalene, Lesen
author_facet	Dalene, Lesen
author_sort	Dalene, Lesen
title	Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi
title_short	Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi
title_full	Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi
title_fullStr	Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi
title_full_unstemmed	Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi
title_sort	screening and biodegradation of polycyclic aromatic hydrocarbons by indigenous endophytic fungi
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2019
url	http://ir.unimas.my/id/eprint/26239/1/Dalene%20anak%20Lesen%20ft.pdf
_version_	1783728307444908032

Screening and Biodegradation of Polycyclic Aromatic Hydrocarbons by Indigenous Endophytic Fungi

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