Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma

Ribosomal proteins (RPs) are components of ribosome involved in ribosome biogenesis. Apart from their classical role, some of these RPs were reported to have extraribosomal functions such as deoxyribonucleic acid (DNA) replication, transcription and apoptosis. However, little is known about the role...

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Main Author:	Chan, Stella Li Li
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Q Science (General) QH Natural history
Online Access:	http://ir.unimas.my/id/eprint/26728/1/Stella%20Chan%20Li%20Li%20ft.pdf
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id	my-unimas-ir.26728
record_format	uketd_dc
spelling	my-unimas-ir.267282023-04-27T02:55:26Z Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma 2016 Chan, Stella Li Li Q Science (General) QH Natural history Ribosomal proteins (RPs) are components of ribosome involved in ribosome biogenesis. Apart from their classical role, some of these RPs were reported to have extraribosomal functions such as deoxyribonucleic acid (DNA) replication, transcription and apoptosis. However, little is known about the role of RPs in tumorigenesis of nasopharyngeal carcinoma (NPC). Previous study has shown that ribosomal protein large subunit L27 (RPeL27), ribosomal protein large subunit L43 (RPeL43) and ribosomal protein large subunit L41 (RPeL41) showed differential expression in cell lines derived from NPC compared to nonnal epithelial (NPE) cell line. Here, the same three RPs were reported overexpressed in both transcript and protein level in five NPC cell lines in relative to one NPE cell line. The gene expression was relatively quantitated using real-time polyn1erase chain reaction (qPCR) while protein expression was detected using Western Blot (WB). Co-immunoprecipitation (co-IP) assay identified RPeL27 and RPeL43 interacting with POTE ankyrin domain family Eltubulin alpha-lA chain (POTEE/TUBAIA) and actin, cytoplasmic Ilbeta-actin-like protein 2 (ACTB/ACTBL2) complex endogenously in HKI while RPeL43 was reported interacting with eukaryotic translation initiation factor 2 subunit 1 (EIF2S 1) and muscle rat sarcoma viral oncogene homolog (MRAS). The differential expression ofthe three above mentioned RPs confinned their involvement in NPc. The identified interacting proteins with RPeL27 and RPeL43 has suggested possible mitogen-activated protein kinase/extracellular signal-related kinase (MAPKIERK) pathway in NPC metastasis. Universiti Malaysia Sarawak (UNIMAS) 2016 Thesis http://ir.unimas.my/id/eprint/26728/ http://ir.unimas.my/id/eprint/26728/1/Stella%20Chan%20Li%20Li%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QH Natural history
spellingShingle	Q Science (General) QH Natural history Chan, Stella Li Li Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma
description	Ribosomal proteins (RPs) are components of ribosome involved in ribosome biogenesis. Apart from their classical role, some of these RPs were reported to have extraribosomal functions such as deoxyribonucleic acid (DNA) replication, transcription and apoptosis. However, little is known about the role of RPs in tumorigenesis of nasopharyngeal carcinoma (NPC). Previous study has shown that ribosomal protein large subunit L27 (RPeL27), ribosomal protein large subunit L43 (RPeL43) and ribosomal protein large subunit L41 (RPeL41) showed differential expression in cell lines derived from NPC compared to nonnal epithelial (NPE) cell line. Here, the same three RPs were reported overexpressed in both transcript and protein level in five NPC cell lines in relative to one NPE cell line. The gene expression was relatively quantitated using real-time polyn1erase chain reaction (qPCR) while protein expression was detected using Western Blot (WB). Co-immunoprecipitation (co-IP) assay identified RPeL27 and RPeL43 interacting with POTE ankyrin domain family Eltubulin alpha-lA chain (POTEE/TUBAIA) and actin, cytoplasmic Ilbeta-actin-like protein 2 (ACTB/ACTBL2) complex endogenously in HKI while RPeL43 was reported interacting with eukaryotic translation initiation factor 2 subunit 1 (EIF2S 1) and muscle rat sarcoma viral oncogene homolog (MRAS). The differential expression ofthe three above mentioned RPs confinned their involvement in NPc. The identified interacting proteins with RPeL27 and RPeL43 has suggested possible mitogen-activated protein kinase/extracellular signal-related kinase (MAPKIERK) pathway in NPC metastasis.
format	Thesis
qualification_level	Master's degree
author	Chan, Stella Li Li
author_facet	Chan, Stella Li Li
author_sort	Chan, Stella Li Li
title	Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma
title_short	Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma
title_full	Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma
title_fullStr	Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma
title_full_unstemmed	Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma
title_sort	association of human rpel27, rpel143 and rpel41 with nasopharyngeal carcinoma
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2016
url	http://ir.unimas.my/id/eprint/26728/1/Stella%20Chan%20Li%20Li%20ft.pdf
_version_	1783728324412964864

Association of Human Rpel27, Rpel143 and Rpel41 with Nasopharyngeal Carcinoma

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