Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome

Enhancers play major roles in the regulation of gene expression at various developmental stages and biological processes temporally and spatially. Albeit the first enhancer was discovered for more than four decades ago, the identification of enhancers is still at its infancy as there is no single un...

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Main Author:	Lim, Whye Kit Leonard
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General)
Online Access:	http://ir.unimas.my/id/eprint/27454/1/Lim%20Whye.pdf
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id	my-unimas-ir.27454
record_format	uketd_dc
spelling	my-unimas-ir.274542023-06-26T08:00:13Z Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome 2019 Lim, Whye Kit Leonard Q Science (General) Enhancers play major roles in the regulation of gene expression at various developmental stages and biological processes temporally and spatially. Albeit the first enhancer was discovered for more than four decades ago, the identification of enhancers is still at its infancy as there is no single universal enhancer mark revealed to date. With the booming emergence of genome sequences of various non-human primates, the study of enhancer landscape across these primates had been made possible recently. Therefore, this study aimed to identify, isolate and characterize enhancers from the genome of one of the most ancient colobine primate, the proboscis monkey (Nasalis larvatus). A total of 13 liver-specific enhancers were identified using three different in silico approaches and they were inserted into expression plasmids before tested further for their activities in HepG2 and A549 cell lines. Two of the strong enhancers and one weak enhancer were subjected to deletion analysis and activity determination subsequently in both cell lines. This study has discovered several clusters of adjacently located transcription factor binding sites that influence enhancer activities and tissue specificities greatly. The results from this research will drive future investigations on the enhancer landscape of the proboscis monkey and comparison of the landscape across different primates. Keywords: Enhancer, identification, proboscis monkey, in silico, expression Universiti Malaysia Sarawak (UNIMAS) 2019 Thesis http://ir.unimas.my/id/eprint/27454/ http://ir.unimas.my/id/eprint/27454/1/Lim%20Whye.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Lim, Whye Kit Leonard Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome
description	Enhancers play major roles in the regulation of gene expression at various developmental stages and biological processes temporally and spatially. Albeit the first enhancer was discovered for more than four decades ago, the identification of enhancers is still at its infancy as there is no single universal enhancer mark revealed to date. With the booming emergence of genome sequences of various non-human primates, the study of enhancer landscape across these primates had been made possible recently. Therefore, this study aimed to identify, isolate and characterize enhancers from the genome of one of the most ancient colobine primate, the proboscis monkey (Nasalis larvatus). A total of 13 liver-specific enhancers were identified using three different in silico approaches and they were inserted into expression plasmids before tested further for their activities in HepG2 and A549 cell lines. Two of the strong enhancers and one weak enhancer were subjected to deletion analysis and activity determination subsequently in both cell lines. This study has discovered several clusters of adjacently located transcription factor binding sites that influence enhancer activities and tissue specificities greatly. The results from this research will drive future investigations on the enhancer landscape of the proboscis monkey and comparison of the landscape across different primates. Keywords: Enhancer, identification, proboscis monkey, in silico, expression
format	Thesis
qualification_level	Master's degree
author	Lim, Whye Kit Leonard
author_facet	Lim, Whye Kit Leonard
author_sort	Lim, Whye Kit Leonard
title	Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome
title_short	Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome
title_full	Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome
title_fullStr	Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome
title_full_unstemmed	Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome
title_sort	identification and characterization of liver-specific enhancer elements in chromosome 18 of the nasalis larvatus genome
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2019
url	http://ir.unimas.my/id/eprint/27454/1/Lim%20Whye.pdf
_version_	1783728334801207296

Identification and Characterization of Liver-Specific Enhancer Elements in Chromosome 18 of the Nasalis larvatus Genome

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