Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)

Rapid clonal propagation of Neolamarckia cadamba (Roxb.) Bosser is significant for large-scale production of planting materials to support the development of planted forests and reduce reliance on natural forests in Malaysia. The genetic stability of regenerated plantlets is crucial to ascertain the...

Full description

Saved in:

Bibliographic Details
Main Author:	Mok, Pei Kieng
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QP Physiology
Online Access:	http://ir.unimas.my/id/eprint/27460/2/Mok%20Pei%20Kieng.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimas-ir.27460
record_format	uketd_dc
spelling	my-unimas-ir.274602023-03-22T09:10:52Z Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan) 2019 Mok, Pei Kieng QP Physiology Rapid clonal propagation of Neolamarckia cadamba (Roxb.) Bosser is significant for large-scale production of planting materials to support the development of planted forests and reduce reliance on natural forests in Malaysia. The genetic stability of regenerated plantlets is crucial to ascertain the quality and uniformity of planting materials for planted forests development. An efficient protocol was established for micropropagation of N. cadamba through direct shoot organogenesis using nodal explants. An average of 5.4 shoots per explant was obtained in B5 medium fortified with 1.0 mg/L BAP. The axillary shoot proliferation was achieved with the number of shoots ranging from 3.5 to 5.4 after subcultured on B5 medium augmented with 0.8 mg/L BAP. Half-strength B5 medium enriched with 0.1 mg/L PBZ was found suitable for rooting with 100% of root induction. The rooted plantlets were successfully acclimatised and transplanted to the greenhouse with more than 95% survival rate. The genetic stability of the regenerants from first, second, third and sixth subcultures of four stock plants was verified by using five random amplified polymorphism DNA (RAPD) and two inter-simple sequence repeats (ISSR) primers along with single nucleotide polymorphism (SNP) analysis by using a gene-specific marker. By using the RAPD and ISSR markers, a total of 9,334 bands and 2,760 bands were amplified respectively, from a total number of 164 tested plants. All of these bands were monomorphic across all in vitro regenerated plantlets. The consensus sequences of Susy gene from plantlets were aligned and compared, however no SNP was detected. These results ascertained the efficiency and reliability of this micropropagation protocol for commercial propagation and germplasm conservation of selected N. cadamba, as well as to facilitate the development of planted forests. Universiti Malaysia Sarawak, (UNIMAS) 2019 Thesis http://ir.unimas.my/id/eprint/27460/ http://ir.unimas.my/id/eprint/27460/2/Mok%20Pei%20Kieng.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QP Physiology
spellingShingle	QP Physiology Mok, Pei Kieng Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)
description	Rapid clonal propagation of Neolamarckia cadamba (Roxb.) Bosser is significant for large-scale production of planting materials to support the development of planted forests and reduce reliance on natural forests in Malaysia. The genetic stability of regenerated plantlets is crucial to ascertain the quality and uniformity of planting materials for planted forests development. An efficient protocol was established for micropropagation of N. cadamba through direct shoot organogenesis using nodal explants. An average of 5.4 shoots per explant was obtained in B5 medium fortified with 1.0 mg/L BAP. The axillary shoot proliferation was achieved with the number of shoots ranging from 3.5 to 5.4 after subcultured on B5 medium augmented with 0.8 mg/L BAP. Half-strength B5 medium enriched with 0.1 mg/L PBZ was found suitable for rooting with 100% of root induction. The rooted plantlets were successfully acclimatised and transplanted to the greenhouse with more than 95% survival rate. The genetic stability of the regenerants from first, second, third and sixth subcultures of four stock plants was verified by using five random amplified polymorphism DNA (RAPD) and two inter-simple sequence repeats (ISSR) primers along with single nucleotide polymorphism (SNP) analysis by using a gene-specific marker. By using the RAPD and ISSR markers, a total of 9,334 bands and 2,760 bands were amplified respectively, from a total number of 164 tested plants. All of these bands were monomorphic across all in vitro regenerated plantlets. The consensus sequences of Susy gene from plantlets were aligned and compared, however no SNP was detected. These results ascertained the efficiency and reliability of this micropropagation protocol for commercial propagation and germplasm conservation of selected N. cadamba, as well as to facilitate the development of planted forests.
format	Thesis
qualification_level	Master's degree
author	Mok, Pei Kieng
author_facet	Mok, Pei Kieng
author_sort	Mok, Pei Kieng
title	Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)
title_short	Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)
title_full	Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)
title_fullStr	Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)
title_full_unstemmed	Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)
title_sort	micropropagation and genetic stability of neolamarckia cadamba (kelampayan)
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2019
url	http://ir.unimas.my/id/eprint/27460/2/Mok%20Pei%20Kieng.pdf
_version_	1783728336094101504

Micropropagation and Genetic Stability of Neolamarckia cadamba (Kelampayan)

Similar Items