Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)

This study involved the isolation of a eDNA and a near oomplete genomic sequence that determine the granule-bound starch synthase {GBSS) gene from sago palm (Metroxylon sagu). The eDNA was isolated via a reverse transcription-polymerase chain reaction (RT -PCR) technique using primers designed bas...

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Main Author:	Lau, Jennifer Siew Kee
Format:	Thesis
Language:	English
Published:	2001
Subjects:	QK Botany
Online Access:	http://ir.unimas.my/id/eprint/27918/2/isolation%20full.pdf
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id	my-unimas-ir.27918
record_format	uketd_dc
spelling	my-unimas-ir.279182023-03-09T07:46:38Z Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu) 2001 Lau, Jennifer Siew Kee QK Botany This study involved the isolation of a eDNA and a near oomplete genomic sequence that determine the granule-bound starch synthase {GBSS) gene from sago palm (Metroxylon sagu). The eDNA was isolated via a reverse transcription-polymerase chain reaction (RT -PCR) technique using primers designed based on the conserved regions of the GBSS. The RT-PCR product obtained, 917 bp in size, was cloned and sequenced. Homology search by using the BLASTX search program confirmed that the RT-PCR product consists of part of the GBSS gene, with the deduced amino acid sequence sharing 68-7:Z01o identities with GBSS of rice, potato, maize, wheat and cassava. A pair of specific internal primers was then designed based on the RT-PCR product. These primers were used to amplify the corresponding genomic DNA region via a genomic PCR method. A 2,008 bp genomic PCR product was generated by this primer set. Cloning and sequencing of the genomic PCR product confinned that it consists of a partial genomic GBSS gene. This genomic PCR product was then used as a probe in an attempt to isolate the entire genomic locus ofthe sago GBSS via Southern hybridization. Universiti Malaysia Sarawak (UNIMAS) 2001 Thesis http://ir.unimas.my/id/eprint/27918/ http://ir.unimas.my/id/eprint/27918/2/isolation%20full.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QK Botany
spellingShingle	QK Botany Lau, Jennifer Siew Kee Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
description	This study involved the isolation of a eDNA and a near oomplete genomic sequence that determine the granule-bound starch synthase {GBSS) gene from sago palm (Metroxylon sagu). The eDNA was isolated via a reverse transcription-polymerase chain reaction (RT -PCR) technique using primers designed based on the conserved regions of the GBSS. The RT-PCR product obtained, 917 bp in size, was cloned and sequenced. Homology search by using the BLASTX search program confirmed that the RT-PCR product consists of part of the GBSS gene, with the deduced amino acid sequence sharing 68-7:Z01o identities with GBSS of rice, potato, maize, wheat and cassava. A pair of specific internal primers was then designed based on the RT-PCR product. These primers were used to amplify the corresponding genomic DNA region via a genomic PCR method. A 2,008 bp genomic PCR product was generated by this primer set. Cloning and sequencing of the genomic PCR product confinned that it consists of a partial genomic GBSS gene. This genomic PCR product was then used as a probe in an attempt to isolate the entire genomic locus ofthe sago GBSS via Southern hybridization.
format	Thesis
qualification_level	Master's degree
author	Lau, Jennifer Siew Kee
author_facet	Lau, Jennifer Siew Kee
author_sort	Lau, Jennifer Siew Kee
title	Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_short	Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_full	Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_fullStr	Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_full_unstemmed	Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)
title_sort	isolation and characterization of near complete cdna and genomic sequences coding for granule-bound starch synthase in sago palm (metroxylon sagu)
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2001
url	http://ir.unimas.my/id/eprint/27918/2/isolation%20full.pdf
_version_	1783728352645873664

Isolation and characterization of near complete cDNA and genomic sequences coding for granule-bound starch synthase in sago palm (Metroxylon sagu)

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