Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)

Adenosine-5'-diphosphoglucose pyrophosphorylase (AGPase) is the allosterically regulated enzyme that catalyzes the rate-limiting step in starch synthesis. Plant AGPases are heterotetrameric with various isoforms exhibiting distinct properties. The objective of this research is to study large s...

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Main Author:	Chong, Patricia Ing Pei
Format:	Thesis
Language:	English
Published:	2008
Subjects:	SB Plant culture
Online Access:	http://ir.unimas.my/id/eprint/28262/1/Patricia%20Chong%20Ing%20Pei%20ft.pdf
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id	my-unimas-ir.28262
record_format	uketd_dc
spelling	my-unimas-ir.282622023-05-10T02:57:33Z Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu) 2008 Chong, Patricia Ing Pei SB Plant culture Adenosine-5'-diphosphoglucose pyrophosphorylase (AGPase) is the allosterically regulated enzyme that catalyzes the rate-limiting step in starch synthesis. Plant AGPases are heterotetrameric with various isoforms exhibiting distinct properties. The objective of this research is to study large subunits of AGPase of sago palm (Metroxylon sagu). In an attempt to learn more about the organization and expression of the small and largesubunit genes of AGPase, I have isolated the partial small and large-subunit gene and cDNA encoding this enzyme in sago palm from the pith and leaf tissues. Through RTPCR screenings of the leaf and pith samples using specific primers that were used to amplify agpll9 and agppl2 respectively, the leaf and pith cDNAs identified by Au and colleagues (2002), similar nucleic acid sequences of agp119 in both leaf and pith cDNAs have been revealed. Semi-quantitative analysis has previously suggested that the large subunit agpl19 was detected only in the leaves of the sago palm (Au et al., 2002). However, this study has managed to isolate, clone and subsequently sequenced cDNAs not only in young and mature leaves but also in the starchy pith revealing the expression of agp119 in both leaf and pith. The 3' end of the partially sequenced agpll9 of leaf has also been identified using RNA Ligase Mediated Rapid Amplification of cDNA Ends (RLM-RACE) and two possible isoforms of agppl2 found in pith have been isolated and subjected to analysis. Universiti Malaysia Sarawak (UNIMAS) 2008 Thesis http://ir.unimas.my/id/eprint/28262/ http://ir.unimas.my/id/eprint/28262/1/Patricia%20Chong%20Ing%20Pei%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Chong, Patricia Ing Pei Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)
description	Adenosine-5'-diphosphoglucose pyrophosphorylase (AGPase) is the allosterically regulated enzyme that catalyzes the rate-limiting step in starch synthesis. Plant AGPases are heterotetrameric with various isoforms exhibiting distinct properties. The objective of this research is to study large subunits of AGPase of sago palm (Metroxylon sagu). In an attempt to learn more about the organization and expression of the small and largesubunit genes of AGPase, I have isolated the partial small and large-subunit gene and cDNA encoding this enzyme in sago palm from the pith and leaf tissues. Through RTPCR screenings of the leaf and pith samples using specific primers that were used to amplify agpll9 and agppl2 respectively, the leaf and pith cDNAs identified by Au and colleagues (2002), similar nucleic acid sequences of agp119 in both leaf and pith cDNAs have been revealed. Semi-quantitative analysis has previously suggested that the large subunit agpl19 was detected only in the leaves of the sago palm (Au et al., 2002). However, this study has managed to isolate, clone and subsequently sequenced cDNAs not only in young and mature leaves but also in the starchy pith revealing the expression of agp119 in both leaf and pith. The 3' end of the partially sequenced agpll9 of leaf has also been identified using RNA Ligase Mediated Rapid Amplification of cDNA Ends (RLM-RACE) and two possible isoforms of agppl2 found in pith have been isolated and subjected to analysis.
format	Thesis
qualification_level	Master's degree
author	Chong, Patricia Ing Pei
author_facet	Chong, Patricia Ing Pei
author_sort	Chong, Patricia Ing Pei
title	Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)
title_short	Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)
title_full	Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)
title_fullStr	Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)
title_full_unstemmed	Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)
title_sort	isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2008
url	http://ir.unimas.my/id/eprint/28262/1/Patricia%20Chong%20Ing%20Pei%20ft.pdf
_version_	1783728358529433600

Isolation, characterization and localization of partial small and large subunits of the adp-glucose pyrophosphorylase gene from sago palm (metroxylon sagu)

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