Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge

Enrichment culture technique lead to the discovery of three presumptive TPHdegrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques revealed that the bacteria isolates were Pseudomonas aeruginosa (LIMAS 1 PF), Serratia marcescens (UMAS2...

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Main Author:	Nur Hafizah, Binti Azizan
Format:	Thesis
Language:	English
Published:	2009
Subjects:	TP Chemical technology
Online Access:	http://ir.unimas.my/id/eprint/28271/1/Nur%20Hafizah%20Azizan%20ft.pdf
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spelling	my-unimas-ir.282712023-06-13T03:45:50Z Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge 2009 Nur Hafizah, Binti Azizan TP Chemical technology Enrichment culture technique lead to the discovery of three presumptive TPHdegrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques revealed that the bacteria isolates were Pseudomonas aeruginosa (LIMAS 1 PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF) with 99%, 98% and 84% nucleotide similarity, respectively. These isolates were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt media supplemented with 1% (v/v) crude oil. Growth study showed that isolates produced the highest cell counts on the third or fourth day by' 108 - 1011 CFU/ml. Six artificial consortia have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 - C24 were recorded. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader as they degraded between 55 - 90% of n-C14 - C, 8 in 14 days. This was followed by UMASIPF and UMAS3KF with 11 - 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2: B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, Al: B2, B+NPK and A2: B3. Result showed that UMAS IPF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2: B3 showed the highest PAHs degradation, followed by plots Al: B2, A3: BI: C2 and Al: C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively. Universiti Malaysia Sarawak (UNIMAS) 2009 Thesis http://ir.unimas.my/id/eprint/28271/ http://ir.unimas.my/id/eprint/28271/1/Nur%20Hafizah%20Azizan%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nur Hafizah, Binti Azizan Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge
description	Enrichment culture technique lead to the discovery of three presumptive TPHdegrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques revealed that the bacteria isolates were Pseudomonas aeruginosa (LIMAS 1 PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF) with 99%, 98% and 84% nucleotide similarity, respectively. These isolates were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt media supplemented with 1% (v/v) crude oil. Growth study showed that isolates produced the highest cell counts on the third or fourth day by' 108 - 1011 CFU/ml. Six artificial consortia have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 - C24 were recorded. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader as they degraded between 55 - 90% of n-C14 - C, 8 in 14 days. This was followed by UMASIPF and UMAS3KF with 11 - 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2: B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, Al: B2, B+NPK and A2: B3. Result showed that UMAS IPF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2: B3 showed the highest PAHs degradation, followed by plots Al: B2, A3: BI: C2 and Al: C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.
format	Thesis
qualification_level	Master's degree
author	Nur Hafizah, Binti Azizan
author_facet	Nur Hafizah, Binti Azizan
author_sort	Nur Hafizah, Binti Azizan
title	Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge
title_short	Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge
title_full	Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge
title_fullStr	Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge
title_full_unstemmed	Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge
title_sort	formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2009
url	http://ir.unimas.my/id/eprint/28271/1/Nur%20Hafizah%20Azizan%20ft.pdf
_version_	1783728359032750080

Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge

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