Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)

Isolation and characterization of secondary metabolites and biological activity screening on five species of soft corals Sinularia brassica, S. frondosa, Sarcophyton glaucum, S. trocheliopherum and Cladiella sp. have been performed, Three compounds of indole derivative have been purified and charac...

Full description

Saved in:

Bibliographic Details
Main Author:	Diana Kertini, Binti Monir
Format:	Thesis
Language:	English
Published:	2009
Subjects:	QL Zoology
Online Access:	http://ir.unimas.my/id/eprint/28275/1/Diana%20Kertini%20binti%20Monir%20ft.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-unimas-ir.28275
record_format	uketd_dc
spelling	my-unimas-ir.282752023-05-11T09:15:28Z Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae) 2009 Diana Kertini, Binti Monir QL Zoology Isolation and characterization of secondary metabolites and biological activity screening on five species of soft corals Sinularia brassica, S. frondosa, Sarcophyton glaucum, S. trocheliopherum and Cladiella sp. have been performed, Three compounds of indole derivative have been purified and characterized from Sinularia frondosa. The compounds have been identified as N-methyl-3,4,5- tribromoindole, N-methyl-3, 5, 6-tribromoindole and 3,5, 6-tribromoindole. N-methyl3, 4, 5-tribromoindole and N-methyl -3, 5, 6-tribromoindole have been isolated by extensive column chromatography techniques using C6H14 CH2Cl2 (hexane dichloromethane) (1 . 1) and preparative thin layer chromatography using C6H14 Et20 (hexane : diethyl ether) (4 1). 3,5,6- Tribromoindole was purled by column chromatography using CH2C12. N-methyl -3, 4, 5-Tribromoindole and N-methyl-3, 5, 6- tribromoindole were obtained as a white crystalline needles with melting point of 116 - 118 °C and 160 - 162 °C, respectively. 3,5, 6-tribromoindole was obtained as a pale brown crystalline needles with melting point of 106 - 108 °C. The structures of all compounds were established based on various spectroscopic techniques such as infrared, nuclear magnetic resonans ('H- AMR, 13C-NMRO and mass spectrometry. ýToxicity, antifungal and antibacterial screening have been performed on all the five species of soft corals studied' sults showed that all the partition of the soft corals were toxic to the brine shrimps Artemia sauna with LC5o value between 1 - 70 pg/mL. Dichloromethane partition of Cladiella sp. and Sarcoph vton trochehopherum, hexane partition of Sarcophvton glaucum and Sarcophvton trochehopherum and ethyl acetate partition of Sarcophvton glaucum and Sarcophvton trochehopherum and ethyl acetate partition of Sarcophvton glaucum and Sarcophvton trocheliopherum gave strong toxicity against brine shrimp Artemia V sauna with LC5o in a range of 1 - 3 ug/mL. Antifungal screening showed that all the partition obtained from the five species of soft coral studied inhibit Asp piker and Fusarium sp. with inhibition zone of 15.29 - 23.56 % and 15.91- 24.92 respectively at concentration of 80 ppm. Ethyl acetate partition from Sinularia frondosa showed strongest inhibition (23.56 %) towards Asperyillus or n while dicholomethane partition from Sinularia brassica showed strongest inhibiton (24.92 %) towards Fusarium sp.. Antibacterial screening showed that all the partition from the five species of soft corals studied exhibited antibacterial activity at least towards five species of bacteria out of 15 species of bacteria tested at a concentration of 2.5 mg/disk. Universiti Malaysia Sarawak (UNIMAS) 2009 Thesis http://ir.unimas.my/id/eprint/28275/ http://ir.unimas.my/id/eprint/28275/1/Diana%20Kertini%20binti%20Monir%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QL Zoology
spellingShingle	QL Zoology Diana Kertini, Binti Monir Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)
description	Isolation and characterization of secondary metabolites and biological activity screening on five species of soft corals Sinularia brassica, S. frondosa, Sarcophyton glaucum, S. trocheliopherum and Cladiella sp. have been performed, Three compounds of indole derivative have been purified and characterized from Sinularia frondosa. The compounds have been identified as N-methyl-3,4,5- tribromoindole, N-methyl-3, 5, 6-tribromoindole and 3,5, 6-tribromoindole. N-methyl3, 4, 5-tribromoindole and N-methyl -3, 5, 6-tribromoindole have been isolated by extensive column chromatography techniques using C6H14 CH2Cl2 (hexane dichloromethane) (1 . 1) and preparative thin layer chromatography using C6H14 Et20 (hexane : diethyl ether) (4 1). 3,5,6- Tribromoindole was purled by column chromatography using CH2C12. N-methyl -3, 4, 5-Tribromoindole and N-methyl-3, 5, 6- tribromoindole were obtained as a white crystalline needles with melting point of 116 - 118 °C and 160 - 162 °C, respectively. 3,5, 6-tribromoindole was obtained as a pale brown crystalline needles with melting point of 106 - 108 °C. The structures of all compounds were established based on various spectroscopic techniques such as infrared, nuclear magnetic resonans ('H- AMR, 13C-NMRO and mass spectrometry. ýToxicity, antifungal and antibacterial screening have been performed on all the five species of soft corals studied' sults showed that all the partition of the soft corals were toxic to the brine shrimps Artemia sauna with LC5o value between 1 - 70 pg/mL. Dichloromethane partition of Cladiella sp. and Sarcoph vton trochehopherum, hexane partition of Sarcophvton glaucum and Sarcophvton trochehopherum and ethyl acetate partition of Sarcophvton glaucum and Sarcophvton trochehopherum and ethyl acetate partition of Sarcophvton glaucum and Sarcophvton trocheliopherum gave strong toxicity against brine shrimp Artemia V sauna with LC5o in a range of 1 - 3 ug/mL. Antifungal screening showed that all the partition obtained from the five species of soft coral studied inhibit Asp piker and Fusarium sp. with inhibition zone of 15.29 - 23.56 % and 15.91- 24.92 respectively at concentration of 80 ppm. Ethyl acetate partition from Sinularia frondosa showed strongest inhibition (23.56 %) towards Asperyillus or n while dicholomethane partition from Sinularia brassica showed strongest inhibiton (24.92 %) towards Fusarium sp.. Antibacterial screening showed that all the partition from the five species of soft corals studied exhibited antibacterial activity at least towards five species of bacteria out of 15 species of bacteria tested at a concentration of 2.5 mg/disk.
format	Thesis
qualification_level	Master's degree
author	Diana Kertini, Binti Monir
author_facet	Diana Kertini, Binti Monir
author_sort	Diana Kertini, Binti Monir
title	Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)
title_short	Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)
title_full	Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)
title_fullStr	Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)
title_full_unstemmed	Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)
title_sort	kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (alcyonaceae)
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2009
url	http://ir.unimas.my/id/eprint/28275/1/Diana%20Kertini%20binti%20Monir%20ft.pdf
_version_	1783728359345225728

Kajian kimia dan penyaringan aktiviti biologi ke atas lima spesis karang lembut (Alcyonaceae)

Similar Items