Molecular phylogenetic of two Bornean Microchiroptera genera Hipposideros and Myotis and phylogeography of Hipposideros cervinus in Sabah and Sarawak
Phylogenetic relationships on Microchiroptera have previously been constructed based upon morphological and behavioral data but to date no one has investigated those relationships using molecular characters. This study reports the results of molecular analysis using sequences from two mitochondri...
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| Format: | Thesis |
| Language: | English |
| Published: |
2007
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/28814/1/Imelda%20Vivian%20Paul%20ft.pdf |
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| Summary: | Phylogenetic relationships on Microchiroptera have previously been constructed based upon
morphological and behavioral data but to date no one has investigated those relationships
using molecular characters. This study reports the results of molecular analysis using
sequences from two mitochondrial genes (12S rRNA and 16S rRNA) on two genera
Hipposideros and Myotis of the Bornean Microchiroptera and also the phylogeography of H
cervinus species in Sabah and Sarawak.
Phylogenetic analysis of the genus Hipposideros was mostly concordant with those of Payne
et a1 (1985) and Corbet and Hill (1992) especially regarding the sister relationships
between H bicolor and Hater and between H larvatus and H diadema. The ML tree
obtained also gave supports for the seven taxonomic species groups of Hipposideros
proposed by Hill (1963). Unfortunately, phylogenetic analysis of the genus Myotis could not
be fully resolved especially the interspecies relationship within this genus. There was also
no support for the subdivision of genus Myotis into two subgenus; Selysius and Leuconoe.
The only strong support was the close relationship between M horsfieldii and M hasseltii.
This present study also utilised PCR-RFLP approach in exammmg the phylogenetic
relationships among the species in the genus Hipposlderos. Although, there are some
disagreements in the phylogenetic tree obtained if compared to sequence analysis,
especially regarding the sister relationship but this method had successfully discovered two
enzymes HindIII and KpnII as being species specific for H dyacorum and Hater. |
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