Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak

In this study, occurrence of Listeria spp., especially Listeria monocylogenes was determined in 440 food samples (poultry, beef and seafood) and 447 of tabletop samples from 11 wet markets in Kuching and Samarahan district, Sarawak. Two enrichment broths; Buffered Listeria Enrichment Broth and Frase...

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Main Author:	Wan Adnawani, Meor Osman
Format:	Thesis
Language:	English
Published:	2007
Subjects:	QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/28841/2/Adnawati.pdf
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id	my-unimas-ir.28841
record_format	uketd_dc
spelling	my-unimas-ir.288412023-05-19T03:09:09Z Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak 2007 Wan Adnawani, Meor Osman QR Microbiology In this study, occurrence of Listeria spp., especially Listeria monocylogenes was determined in 440 food samples (poultry, beef and seafood) and 447 of tabletop samples from 11 wet markets in Kuching and Samarahan district, Sarawak. Two enrichment broths; Buffered Listeria Enrichment Broth and Fraser Broth were used in the isolation of L. monocylogenes followed by plating on selective PALCAM agar. Suspected colonies (black with grey-green zone) were picked and then streaked on the CllROMagar Listeria, a chromogenic medium for detection and isolation of L monocylogenes from other Listeria spp. A total of' 82 isolates were identified as L. monocylogenes by the formation of blue colonies with white halo on this agar. All positive isolates were confirmed using species-specific PC R. Based on the presence of the hlyA gene, the 82 isolates were confirmed as L. monocytogenes. The result showed 16 (3.6%) of the food samples and 11 (2.5%) of' the tabletop samples were positive for the occurrence of L. monocytogenes. This revealed a low occurrence of L. monocylogene. s in food and tabletop samples. All 82 isolates were further studied to determine their antibiotic resistance and occurrence of plasmid. L. monocvlogenes isolates were resistant to most antibiotics tested (nalidixic acid (100%), streptomycin (95.1%), ceftriaxone (89%), vancomycin (36.6%), chlorampenicol (12.2%), methacillin (12.2%), gentamicin (11%). kanamycin (8.5%), cephalothin (7.3%). tetracycline (7.3%) and erythromycin (3.7%)). However, all isolates were susceptible towards 3 antibiotics; ampicillin, hacitracin and carbenicillin. None of the L. monocivogencs isolates harboured plasmid. The results of' antibiotyping and plasmid profiles showed that no relationship could he established between the isolates. Screening for presence of the virulence genes (inWA gene, i(q) gene, pr/A gene and picA gene), 60 isolates were selected from food (30) and tabletop (30) samples. All the isolates tested showed positive results for the presence ofiap gene and pr1A gene. Universiti Malaysia Sarawak (UNIMAS) 2007 Thesis http://ir.unimas.my/id/eprint/28841/ http://ir.unimas.my/id/eprint/28841/2/Adnawati.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Wan Adnawani, Meor Osman Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak
description	In this study, occurrence of Listeria spp., especially Listeria monocylogenes was determined in 440 food samples (poultry, beef and seafood) and 447 of tabletop samples from 11 wet markets in Kuching and Samarahan district, Sarawak. Two enrichment broths; Buffered Listeria Enrichment Broth and Fraser Broth were used in the isolation of L. monocylogenes followed by plating on selective PALCAM agar. Suspected colonies (black with grey-green zone) were picked and then streaked on the CllROMagar Listeria, a chromogenic medium for detection and isolation of L monocylogenes from other Listeria spp. A total of' 82 isolates were identified as L. monocylogenes by the formation of blue colonies with white halo on this agar. All positive isolates were confirmed using species-specific PC R. Based on the presence of the hlyA gene, the 82 isolates were confirmed as L. monocytogenes. The result showed 16 (3.6%) of the food samples and 11 (2.5%) of' the tabletop samples were positive for the occurrence of L. monocytogenes. This revealed a low occurrence of L. monocylogene. s in food and tabletop samples. All 82 isolates were further studied to determine their antibiotic resistance and occurrence of plasmid. L. monocvlogenes isolates were resistant to most antibiotics tested (nalidixic acid (100%), streptomycin (95.1%), ceftriaxone (89%), vancomycin (36.6%), chlorampenicol (12.2%), methacillin (12.2%), gentamicin (11%). kanamycin (8.5%), cephalothin (7.3%). tetracycline (7.3%) and erythromycin (3.7%)). However, all isolates were susceptible towards 3 antibiotics; ampicillin, hacitracin and carbenicillin. None of the L. monocivogencs isolates harboured plasmid. The results of' antibiotyping and plasmid profiles showed that no relationship could he established between the isolates. Screening for presence of the virulence genes (inWA gene, i(q) gene, pr/A gene and picA gene), 60 isolates were selected from food (30) and tabletop (30) samples. All the isolates tested showed positive results for the presence ofiap gene and pr1A gene.
format	Thesis
qualification_level	Master's degree
author	Wan Adnawani, Meor Osman
author_facet	Wan Adnawani, Meor Osman
author_sort	Wan Adnawani, Meor Osman
title	Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak
title_short	Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak
title_full	Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak
title_fullStr	Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak
title_full_unstemmed	Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak
title_sort	isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in kuching and samarahan divisions, sarawak
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2007
url	http://ir.unimas.my/id/eprint/28841/2/Adnawati.pdf
_version_	1783728364509462528

Isolation, phenotyping and genotyping of listeria monocytogenes from food and wet markets in Kuching and Samarahan divisions, Sarawak

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