Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells

Ribosomal proteins (RPs) were only believed to involve in ribosome biogenesis and protein synthesis but there are increasing evidences to show the potential extraribosomal functions of ribosomal proteins. Ribosomal proteins are involved in regulation of cellular cell growth and differentiation as ma...

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Main Author:	Yew, Keh Li
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General) QH301 Biology QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/29074/2/Yew%28fulltext%29.pdf
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id	my-unimas-ir.29074
record_format	uketd_dc
spelling	my-unimas-ir.290742023-05-25T07:52:57Z Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells 2019-12-22 Yew, Keh Li Q Science (General) QH301 Biology QR Microbiology Ribosomal proteins (RPs) were only believed to involve in ribosome biogenesis and protein synthesis but there are increasing evidences to show the potential extraribosomal functions of ribosomal proteins. Ribosomal proteins are involved in regulation of cellular cell growth and differentiation as many studies have linked cancers to the differential expression of RPs. The ribosomal protein, eL27 has shown significant differential expression in cell lines derived from nasopharyngeal carcinoma (NPC) as compared to normal nasopharyngeal epithelium at both transcript and protein level. However, the protein-interacting partners of RPeL27 in NPC remains largely unknown. Hence, in this study we identified and characterized the proteins affected by RPeL27-knockdown in NPC via siRNA knockdown, 2-Dimensional Gel Electrophoresis (2D GE), liquid chromatography mass spectrometry (LCMS) and bioinformatics analysis. We successfully identified 15 differentially expressed proteins in RPeL27-knockdown HK1 cells and all the 15 proteins are cancer-related, often dysregulated in various diseases and take part in significant biological processes responsible for proper cell growth and proliferation. Therefore, the overexpression of RPeL27 could be responsible for NPC development and progression through association with 15 proteins identified either directly or indirectly. We predicted the possible protein interactions between RPeL27 and the 15 proteins identified via in silico approach. Our findings reinforce the potential role of ribosomal protein L27 in the carcinogenesis of NPC and provided the possible protein interactions between RPeL27 and 15 proteins. Universiti Malaysia Sarawak (UNIMAS) 2019-12 Thesis http://ir.unimas.my/id/eprint/29074/ http://ir.unimas.my/id/eprint/29074/2/Yew%28fulltext%29.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QH301 Biology QR Microbiology
spellingShingle	Q Science (General) QH301 Biology QR Microbiology Yew, Keh Li Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells
description	Ribosomal proteins (RPs) were only believed to involve in ribosome biogenesis and protein synthesis but there are increasing evidences to show the potential extraribosomal functions of ribosomal proteins. Ribosomal proteins are involved in regulation of cellular cell growth and differentiation as many studies have linked cancers to the differential expression of RPs. The ribosomal protein, eL27 has shown significant differential expression in cell lines derived from nasopharyngeal carcinoma (NPC) as compared to normal nasopharyngeal epithelium at both transcript and protein level. However, the protein-interacting partners of RPeL27 in NPC remains largely unknown. Hence, in this study we identified and characterized the proteins affected by RPeL27-knockdown in NPC via siRNA knockdown, 2-Dimensional Gel Electrophoresis (2D GE), liquid chromatography mass spectrometry (LCMS) and bioinformatics analysis. We successfully identified 15 differentially expressed proteins in RPeL27-knockdown HK1 cells and all the 15 proteins are cancer-related, often dysregulated in various diseases and take part in significant biological processes responsible for proper cell growth and proliferation. Therefore, the overexpression of RPeL27 could be responsible for NPC development and progression through association with 15 proteins identified either directly or indirectly. We predicted the possible protein interactions between RPeL27 and the 15 proteins identified via in silico approach. Our findings reinforce the potential role of ribosomal protein L27 in the carcinogenesis of NPC and provided the possible protein interactions between RPeL27 and 15 proteins.
format	Thesis
qualification_level	Master's degree
author	Yew, Keh Li
author_facet	Yew, Keh Li
author_sort	Yew, Keh Li
title	Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells
title_short	Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells
title_full	Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells
title_fullStr	Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells
title_full_unstemmed	Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells
title_sort	identification and characterization of proteins in association with ribosomal protein, el27 in nasopharyngeal carcinoma cells
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2019
url	http://ir.unimas.my/id/eprint/29074/2/Yew%28fulltext%29.pdf
_version_	1783728368440573952

Identification and Characterization of Proteins in Association with Ribosomal Protein, eL27 in Nasopharyngeal Carcinoma Cells

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