Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)

Sago palm is an important agricultural starch producing plant which contributes to the economics of Malaysia where the trunk is the main plant part that responsible for the production of the starch. However, there are sago palms that failed to develop its trunk after 17 years have been planted. This...

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Main Author:	Yan, Wei Jei
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QP Physiology
Online Access:	http://ir.unimas.my/id/eprint/30670/2/Wei%20Jei%20%28fulltext%29.pdf
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id	my-unimas-ir.30670
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spelling	my-unimas-ir.306702023-05-25T09:19:23Z Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage) 2016 Yan, Wei Jei QP Physiology Sago palm is an important agricultural starch producing plant which contributes to the economics of Malaysia where the trunk is the main plant part that responsible for the production of the starch. However, there are sago palms that failed to develop its trunk after 17 years have been planted. This phenomenon is known as 'non-trunking' sago palm, which eliminates the economic value of the plants. Therefore, the objective of the current study was to determine metabolites that related to the non-trunking characteristics. Metabolites present in a leaf of non-trunking and trunking sago palms were extracted using the Sobolev, multiple solvent and Jones & Kinghorn extraction methods. Subsequently, the extracts were analysed using nuclear magnetic resonance spectroscopy (NMR) and gas chromatography - mass spectrometry (GC-MS). Data obtained were subjected to principal component analysis (PCA). Results showed that the Kinghorn & John extraction method was the best extraction method because it provides lower complexity NMR spectra and its PCA analysis shows the lowest significant non-correlation score of r = 0.1818 between the phenotypes. Metabolite groups which are differently express between trunking and non-trunking sago palm are oils and waxes, haloalkanes, sulfite esters, phosphonates, phosphoric acid, thiophene ester, terpenes and tocopherols. GC-MS analysis of Kinghorn & Jones extraction methods determine two sets of metabolite markers which explains the differences in metabolites expression of trunking and non-trunking sago palm in ethyl acetate and methanol extract of 89.55% comprising sulfurous ester compounds and 87.04% comprising sulfurous ester, sulfurous acid and cyclohexylmethyl hexyl ester respectively. Universiti Malaysia Sarawak(UNIMAS) 2016 Thesis http://ir.unimas.my/id/eprint/30670/ http://ir.unimas.my/id/eprint/30670/2/Wei%20Jei%20%28fulltext%29.pdf text en validuser masters Universiti Malaysia Sarawak(UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QP Physiology
spellingShingle	QP Physiology Yan, Wei Jei Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)
description	Sago palm is an important agricultural starch producing plant which contributes to the economics of Malaysia where the trunk is the main plant part that responsible for the production of the starch. However, there are sago palms that failed to develop its trunk after 17 years have been planted. This phenomenon is known as 'non-trunking' sago palm, which eliminates the economic value of the plants. Therefore, the objective of the current study was to determine metabolites that related to the non-trunking characteristics. Metabolites present in a leaf of non-trunking and trunking sago palms were extracted using the Sobolev, multiple solvent and Jones & Kinghorn extraction methods. Subsequently, the extracts were analysed using nuclear magnetic resonance spectroscopy (NMR) and gas chromatography - mass spectrometry (GC-MS). Data obtained were subjected to principal component analysis (PCA). Results showed that the Kinghorn & John extraction method was the best extraction method because it provides lower complexity NMR spectra and its PCA analysis shows the lowest significant non-correlation score of r = 0.1818 between the phenotypes. Metabolite groups which are differently express between trunking and non-trunking sago palm are oils and waxes, haloalkanes, sulfite esters, phosphonates, phosphoric acid, thiophene ester, terpenes and tocopherols. GC-MS analysis of Kinghorn & Jones extraction methods determine two sets of metabolite markers which explains the differences in metabolites expression of trunking and non-trunking sago palm in ethyl acetate and methanol extract of 89.55% comprising sulfurous ester compounds and 87.04% comprising sulfurous ester, sulfurous acid and cyclohexylmethyl hexyl ester respectively.
format	Thesis
qualification_level	Master's degree
author	Yan, Wei Jei
author_facet	Yan, Wei Jei
author_sort	Yan, Wei Jei
title	Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)
title_short	Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)
title_full	Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)
title_fullStr	Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)
title_full_unstemmed	Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)
title_sort	metabolites comparison between trunking and non-trunking sago palm (metroxylon sage)
granting_institution	Universiti Malaysia Sarawak(UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2016
url	http://ir.unimas.my/id/eprint/30670/2/Wei%20Jei%20%28fulltext%29.pdf
_version_	1783728390869614592

Metabolites comparison between trunking and non-trunking sago palm (Metroxylon sage)

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