Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma

Nasopharyngeal carcinoma (NPC) is currently the fourth most common cancer in Malaysia. Ribosomal protein genes are crucial in producing functional ribosomes that assist in cell functions and have shown to be associated in pathways that link to the formation of cancer. Previous studies have shown tha...

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Main Author:	Chee, Cassandra Sheau Mei
Format:	Thesis
Language:	English English
Published:	2020
Subjects:	Q Science (General) QR180 Immunology
Online Access:	http://ir.unimas.my/id/eprint/31512/1/Mei%2824%29.pdf http://ir.unimas.my/id/eprint/31512/4/Cassandra%20Chee%20Sheau%20Mei%20ft.pdf
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id	my-unimas-ir.31512
record_format	uketd_dc
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English English
topic	Q Science (General) QR180 Immunology
spellingShingle	Q Science (General) QR180 Immunology Chee, Cassandra Sheau Mei Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma
description	Nasopharyngeal carcinoma (NPC) is currently the fourth most common cancer in Malaysia. Ribosomal protein genes are crucial in producing functional ribosomes that assist in cell functions and have shown to be associated in pathways that link to the formation of cancer. Previous studies have shown that uS19 gene is involved in the Mdm2-p53-MdmX pathway, where it inhibits the p53, a tumor suppressor. Furthermore, expression of ribosomal proteins have been studied to observe its expression levels, in which potential biomarkers could be discovered that assist in the early diagnosis of cancers. Notably, uS19 is currently understudied, though studies have been done on the expression of uS19 gene in NPC, where it was found to be upregulated in poorly differentiated NPC tissue samples and HK1 cell line. However, further validation is required. Studies on the post-translational effect of uS19 in NPC carcinogenesis are yet to be done. In this study, mRNA and protein expression of uS19 data obtained revealed a differential expression at both gene and protein levels in NPC cell lines compared to normal nasopharyngeal epithelial (NPE) cells. The gene expression of uS19 was found to be down-regulated following a trend of cell type and differentiation, while protein expression of uS19 was found to be upregulated in all NPC cell lines, with C666-1 cell line being the highest. The data attained showed that uS19 might be involved in the NPC tumorigenesis based on the difference in transcription and translational level. Protein-protein interaction via in silico approach revealed uS19 could possibly bind with 10 proteins, which includes LMP2 and EBNA1, a known Epstein-Barr virus (EBV) protein. Our findings strengthen the potential role of uS19 in NPC tumorigenesis based on the expression data obtained and calculated interactions through bioinformatics analysis that revealed a high possible chance of uS19 protein interacting with EBV proteins involved in the carcinogenesis of NPC.
format	Thesis
qualification_level	Master's degree
author	Chee, Cassandra Sheau Mei
author_facet	Chee, Cassandra Sheau Mei
author_sort	Chee, Cassandra Sheau Mei
title	Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma
title_short	Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma
title_full	Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma
title_fullStr	Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma
title_full_unstemmed	Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma
title_sort	expression analysis of us19 and its association with nasopharyngeal carcinoma
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2020
url	http://ir.unimas.my/id/eprint/31512/1/Mei%2824%29.pdf http://ir.unimas.my/id/eprint/31512/4/Cassandra%20Chee%20Sheau%20Mei%20ft.pdf
_version_	1783728404761149440
spelling	my-unimas-ir.315122023-04-18T02:56:37Z Expression Analysis of uS19 and its Association with Nasopharyngeal Carcinoma 2020-09-01 Chee, Cassandra Sheau Mei Q Science (General) QR180 Immunology Nasopharyngeal carcinoma (NPC) is currently the fourth most common cancer in Malaysia. Ribosomal protein genes are crucial in producing functional ribosomes that assist in cell functions and have shown to be associated in pathways that link to the formation of cancer. Previous studies have shown that uS19 gene is involved in the Mdm2-p53-MdmX pathway, where it inhibits the p53, a tumor suppressor. Furthermore, expression of ribosomal proteins have been studied to observe its expression levels, in which potential biomarkers could be discovered that assist in the early diagnosis of cancers. Notably, uS19 is currently understudied, though studies have been done on the expression of uS19 gene in NPC, where it was found to be upregulated in poorly differentiated NPC tissue samples and HK1 cell line. However, further validation is required. Studies on the post-translational effect of uS19 in NPC carcinogenesis are yet to be done. In this study, mRNA and protein expression of uS19 data obtained revealed a differential expression at both gene and protein levels in NPC cell lines compared to normal nasopharyngeal epithelial (NPE) cells. The gene expression of uS19 was found to be down-regulated following a trend of cell type and differentiation, while protein expression of uS19 was found to be upregulated in all NPC cell lines, with C666-1 cell line being the highest. The data attained showed that uS19 might be involved in the NPC tumorigenesis based on the difference in transcription and translational level. Protein-protein interaction via in silico approach revealed uS19 could possibly bind with 10 proteins, which includes LMP2 and EBNA1, a known Epstein-Barr virus (EBV) protein. Our findings strengthen the potential role of uS19 in NPC tumorigenesis based on the expression data obtained and calculated interactions through bioinformatics analysis that revealed a high possible chance of uS19 protein interacting with EBV proteins involved in the carcinogenesis of NPC. Universiti Malaysia Sarawak (UNIMAS) 2020-09 Thesis http://ir.unimas.my/id/eprint/31512/ http://ir.unimas.my/id/eprint/31512/1/Mei%2824%29.pdf text en public http://ir.unimas.my/id/eprint/31512/4/Cassandra%20Chee%20Sheau%20Mei%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology Arendt, J. F. B., Pederson, L., Nexo, E., Sorensen, H. T. (2013). Elevated plasma vitamin B12 level as a marker for cancer: A population-based cohort. 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