Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds

Heterocyclic hydrocarbon compounds are known as by-products for various industries and has become a threat of contamination in the environment. Bioremediation was applied to solve this problem. However, high toxicity levels tend to inhibit the performance of the bacteria for biodegradation of the co...

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Main Author:	Intan Mariana, Maliki
Format:	Thesis
Language:	English
Published:	2020
Subjects:	QR Microbiology TP Chemical technology
Online Access:	http://ir.unimas.my/id/eprint/33571/1/Intan.pdf
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spelling	my-unimas-ir.335712023-05-10T06:25:59Z Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds 2020-12-23 Intan Mariana, Maliki QR Microbiology TD Environmental technology. Sanitary engineering TP Chemical technology Heterocyclic hydrocarbon compounds are known as by-products for various industries and has become a threat of contamination in the environment. Bioremediation was applied to solve this problem. However, high toxicity levels tend to inhibit the performance of the bacteria for biodegradation of the compounds. Cell immobilization technique was developed and applied to resolve this issue. This study aimed to develop and investigate the performance of immobilized cell in comparison to free cells in the degradation of heterocyclic hydrocarbon compounds such as carbazole (CAR), dibenzofuran (DBF), dibenzothiophene (DBT) and fluorene (FL). In this study, the optimal cell immobilization conditions of isolated marine bacteria, T. profundimaris strain M02 was immobilized by using calcium alginate at 4% (w/v) concentration with cell mass loading of 1.25 g. These developed immobilized cells were also able to be used repetitively although depletion of the cell’s performance was observed. On other hand, T. profundimaris strain M02 was able to degrade multiple heterocyclic hydrocarbon compounds and showed significant performance improvement when immobilized. Actual performance test was carried out in bioreactor and from the test, both free and immobilized cells showed improved performance in a controlled environment in the bioreactor when compared to the laboratory scale experiment. Comparative study of free and immobilized cell performance in bioreactor showed that immobilized cells exhibited higher concentration of CAR degraded as well as higher specific growth rate. To conclude, the findings of this study discovered the optimum conditions for bacteria immobilization for heterocyclic hydrocarbon compounds degradation as well as revealing that a single bacteria strain are able to degrade multiple compounds. This information is important as it can be utilized for biodegradation of heterocyclic hydrocarbon compounds in the environment and improved the existing technique that is currently implemented today. Universiti Malaysia Sarawak (UNIMAS) 2020-12 Thesis http://ir.unimas.my/id/eprint/33571/ http://ir.unimas.my/id/eprint/33571/1/Intan.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Engineering
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QR Microbiology QR Microbiology TP Chemical technology
spellingShingle	QR Microbiology QR Microbiology TP Chemical technology Intan Mariana, Maliki Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds
description	Heterocyclic hydrocarbon compounds are known as by-products for various industries and has become a threat of contamination in the environment. Bioremediation was applied to solve this problem. However, high toxicity levels tend to inhibit the performance of the bacteria for biodegradation of the compounds. Cell immobilization technique was developed and applied to resolve this issue. This study aimed to develop and investigate the performance of immobilized cell in comparison to free cells in the degradation of heterocyclic hydrocarbon compounds such as carbazole (CAR), dibenzofuran (DBF), dibenzothiophene (DBT) and fluorene (FL). In this study, the optimal cell immobilization conditions of isolated marine bacteria, T. profundimaris strain M02 was immobilized by using calcium alginate at 4% (w/v) concentration with cell mass loading of 1.25 g. These developed immobilized cells were also able to be used repetitively although depletion of the cell’s performance was observed. On other hand, T. profundimaris strain M02 was able to degrade multiple heterocyclic hydrocarbon compounds and showed significant performance improvement when immobilized. Actual performance test was carried out in bioreactor and from the test, both free and immobilized cells showed improved performance in a controlled environment in the bioreactor when compared to the laboratory scale experiment. Comparative study of free and immobilized cell performance in bioreactor showed that immobilized cells exhibited higher concentration of CAR degraded as well as higher specific growth rate. To conclude, the findings of this study discovered the optimum conditions for bacteria immobilization for heterocyclic hydrocarbon compounds degradation as well as revealing that a single bacteria strain are able to degrade multiple compounds. This information is important as it can be utilized for biodegradation of heterocyclic hydrocarbon compounds in the environment and improved the existing technique that is currently implemented today.
format	Thesis
qualification_level	Master's degree
author	Intan Mariana, Maliki
author_facet	Intan Mariana, Maliki
author_sort	Intan Mariana, Maliki
title	Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds
title_short	Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds
title_full	Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds
title_fullStr	Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds
title_full_unstemmed	Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds
title_sort	immobilization of thalassospira profundimaris m02 for improved biotransformation of heterocyclic hydrocarbon compounds
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Engineering
publishDate	2020
url	http://ir.unimas.my/id/eprint/33571/1/Intan.pdf
_version_	1783728425850109952

Immobilization of Thalassospira profundimaris M02 for Improved Biotransformation of Heterocyclic Hydrocarbon Compounds

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