Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM
The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the dir...
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| 格式: | Thesis |
| 语言: | English |
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2008
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my-unimas-ir.35882023-04-13T03:59:19Z Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM 2008 Bay, Hui Han QK Botany The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the direct regeneration pathway. The primers produced amplification products which were monomorphic across the mother plants and the micropropagated plantlets, confirming the reliability of developed micropropagation protocol to produce true-to-tpe plantlets. Both leaves and leaf sheaths were used for callus induction in the indirect regeneration pathway. Universiti Malaysia Sarawak, (UNIMAS) 2008 Thesis http://ir.unimas.my/id/eprint/3588/ http://ir.unimas.my/id/eprint/3588/1/Bay%20Hui%20Han%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Institute of Biodiversity and Environmental Conservation. |
| institution |
Universiti Malaysia Sarawak |
| collection |
UNIMAS Institutional Repository |
| language |
English |
| topic |
QK Botany |
| spellingShingle |
QK Botany Bay, Hui Han Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM |
| description |
The axenic seedlings were kept in vitro as the source axenic explants. Shoot-tip explants derived from axenic off shoots were cultured in Gamborg B5 media incorporated with 30% sucrose, 3 g/L gelrite and different concentrations of cytokinins i.e. TDZ, BAP alone or in combination with NAA in the direct regeneration pathway. The primers produced amplification products which were monomorphic across the mother plants and the micropropagated plantlets, confirming the reliability of developed micropropagation protocol to produce true-to-tpe plantlets. Both leaves and leaf sheaths were used for callus induction in the indirect regeneration pathway. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Bay, Hui Han |
| author_facet |
Bay, Hui Han |
| author_sort |
Bay, Hui Han |
| title |
Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM |
| title_short |
Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM |
| title_full |
Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM |
| title_fullStr |
Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM |
| title_full_unstemmed |
Micropropagation and monitoring of genetic stability of micropagated plantlets of Hornstedtia reticulata K. SCHUM |
| title_sort |
micropropagation and monitoring of genetic stability of micropagated plantlets of hornstedtia reticulata k. schum |
| granting_institution |
Universiti Malaysia Sarawak (UNIMAS) |
| granting_department |
Institute of Biodiversity and Environmental Conservation. |
| publishDate |
2008 |
| url |
http://ir.unimas.my/id/eprint/3588/1/Bay%20Hui%20Han%20ft.pdf |
| _version_ |
1783727935373443072 |