Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)

The gut microbiota is known as an ‘extra organ’ and is essential in assisting the host in terms of nutrition and immunity. Gut inflammation associated with bloom of Enterobacteriaceae and overgrowth of existing and invasion of pathogenic bacteria is mainly due to alteration in gut microbial commun...

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Main Author:	Lau, Melinda Mei Lin
Format:	Thesis
Language:	English
Published:	2021
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/36559/1/Melinda%20%20ft.pdf
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id	my-unimas-ir.36559
record_format	uketd_dc
spelling	my-unimas-ir.365592023-09-15T06:33:54Z Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides) 2021 Lau, Melinda Mei Lin Q Science (General) QR Microbiology The gut microbiota is known as an ‘extra organ’ and is essential in assisting the host in terms of nutrition and immunity. Gut inflammation associated with bloom of Enterobacteriaceae and overgrowth of existing and invasion of pathogenic bacteria is mainly due to alteration in gut microbial community. This will eventually increases the disease occurrence within aquaculture farms which bring huge economic loses. However, the possible opportunistic pathogens causing bacterial disease is still in vague. Thus, in this study, the gut microbial community of both diseased and healthy Malaysian mahseer (T. tambroides) were compared and explored using NGS-based 16S rDNA sequencing on the Malaysian mahseer. Three healthy and diseased adult Malaysian mahseers (showing signs of exophthalmia, coelomic distension and petechial haemorrhage) were obtained from LTT Aquaculture Sdn Bhd. Our results revealed significant differences in microbial diversity, composition and function between both populations of T. tambroides. Alpha diversity analysis depicts lower diversity of gut microbiota composition in diseased T. tambroides as compared to the healthy group, which is in coherent with diversity resistance hypothesis, indicating greater chances of occurrence for a species with antagonistic trait against pathogens. Analysis of Composition of Microbiomes (ANCOM) was performed to test on the significant differences at genus and phylum level among the gut microbiota between both groups. In particular, Enterobacteriaceae, Aeromonas, Bacteroides, Vibrio, Arcobacter and Pseudomonas were found within gut microbiota of the diseased fishes. In contrast, Sphingomonadaceae, Methylobacterium, unclassified bacteria and Ruminococcaceae were identified from the gut microbial community of the healthy group. Besides, cellulose-degrading bacteria and protease-producing bacteria were identified from the gut of T. tambroides as well. Taken together, our findings emphasised the association between the alteration in gut microbiota composition and infectious abdominal dropsy in T. tambroides, hoping to aid in diagnosis, prevention and treatment of intestinal diseases in fish. Universiti Malaysia Sarawak (UNIMAS) 2021 Thesis http://ir.unimas.my/id/eprint/36559/ http://ir.unimas.my/id/eprint/36559/1/Melinda%20%20ft.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Lau, Melinda Mei Lin Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)
description	The gut microbiota is known as an ‘extra organ’ and is essential in assisting the host in terms of nutrition and immunity. Gut inflammation associated with bloom of Enterobacteriaceae and overgrowth of existing and invasion of pathogenic bacteria is mainly due to alteration in gut microbial community. This will eventually increases the disease occurrence within aquaculture farms which bring huge economic loses. However, the possible opportunistic pathogens causing bacterial disease is still in vague. Thus, in this study, the gut microbial community of both diseased and healthy Malaysian mahseer (T. tambroides) were compared and explored using NGS-based 16S rDNA sequencing on the Malaysian mahseer. Three healthy and diseased adult Malaysian mahseers (showing signs of exophthalmia, coelomic distension and petechial haemorrhage) were obtained from LTT Aquaculture Sdn Bhd. Our results revealed significant differences in microbial diversity, composition and function between both populations of T. tambroides. Alpha diversity analysis depicts lower diversity of gut microbiota composition in diseased T. tambroides as compared to the healthy group, which is in coherent with diversity resistance hypothesis, indicating greater chances of occurrence for a species with antagonistic trait against pathogens. Analysis of Composition of Microbiomes (ANCOM) was performed to test on the significant differences at genus and phylum level among the gut microbiota between both groups. In particular, Enterobacteriaceae, Aeromonas, Bacteroides, Vibrio, Arcobacter and Pseudomonas were found within gut microbiota of the diseased fishes. In contrast, Sphingomonadaceae, Methylobacterium, unclassified bacteria and Ruminococcaceae were identified from the gut microbial community of the healthy group. Besides, cellulose-degrading bacteria and protease-producing bacteria were identified from the gut of T. tambroides as well. Taken together, our findings emphasised the association between the alteration in gut microbiota composition and infectious abdominal dropsy in T. tambroides, hoping to aid in diagnosis, prevention and treatment of intestinal diseases in fish.
format	Thesis
qualification_level	Master's degree
author	Lau, Melinda Mei Lin
author_facet	Lau, Melinda Mei Lin
author_sort	Lau, Melinda Mei Lin
title	Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)
title_short	Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)
title_full	Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)
title_fullStr	Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)
title_full_unstemmed	Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)
title_sort	metagenomic analysis of gut bacterial communities of healthy and diseased empurau (tor tambroides)
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2021
url	http://ir.unimas.my/id/eprint/36559/1/Melinda%20%20ft.pdf
_version_	1783728474082508800

Metagenomic Analysis of Gut Bacterial Communities of Healthy and Diseased Empurau (Tor tambroides)

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