Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris

Laccases are multipurpose enzymes that have wide biotechnological applications. The present study seeks to evaluate the feasibility of spent fermentation broth generated upon the bioethanol production from sago hampas, as a feedstock for production of laccases in recombinant Pichia pastoris GS115. C...

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Main Author:	Farah Wahida, Mamat
Format:	Thesis
Language:	English English
Published:	2022
Subjects:	Q Science (General) QD Chemistry TP Chemical technology
Online Access:	http://ir.unimas.my/id/eprint/38082/1/Farah%20Wahida%20Binti%20Mamat%20-%2024%20pgs.pdf http://ir.unimas.my/id/eprint/38082/4/Farah%20Wahida.pdf
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spelling	my-unimas-ir.380822023-03-13T08:16:34Z Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris 2022-04-27 Farah Wahida, Mamat Q Science (General) QD Chemistry TP Chemical technology Laccases are multipurpose enzymes that have wide biotechnological applications. The present study seeks to evaluate the feasibility of spent fermentation broth generated upon the bioethanol production from sago hampas, as a feedstock for production of laccases in recombinant Pichia pastoris GS115. Characterisation of the sago bioethanol liquid waste (SBLW) indicated glycerol as the main component, along with glucose and lactic acid. Evaluation of SBLW as a fermentation feedstock for laccase production in P. pastoris GS115 showed that the cell growth was generally feasible when SBLW was used as a feedstock. The activity of laccases reached the highest (0.00076 U mL-1 ± 3.5 × 10-5) in fermentations that employed 40% (v/v) SBLW. This represented 73% of that obtained using the standard synthetic medium. Supplementation of 40% (v/v) SBLW with 1.0% (w/v) yeast extract (YE) yielded enhancements of 1.2-fold and 1.5-fold of biomass concentration and laccase activity, respectively. The expression of laccases was further enhanced when the 40% (v/v) SBLW and 1.0% (w/v) YE was supplemented with 2.0% (v/v) glycerol. The highest laccase activity recorded was 0.00206 U mL-1 ± 5.8 × 10-5 . Both of the biomass and laccase production were increased by 1.9-fold and 2.1-fold in comparison to that achieved by the standard synthetic medium. The efficacy of laccases produced by the optimised SBLW medium was evaluated in terms of the capability of the enzymes to decolourise Remazol Brilliant Blue R (RBBR) dye. The enzyme showed a decolourisation percentage of 68.6%, which represented 91% of the decolourising capability of laccases produced using standard BMMH medium. This indicates the promising efficacy of laccases produced from SBLW. In summary, this work gives an important insight into exploitation of SBLW for production of value-added products. Moreover, this work contributes to the development of recombinant laccase production using low cost and eco-friendly feedstock. Universiti Malaysia Sarawak (UNIMAS) 2022-04 Thesis http://ir.unimas.my/id/eprint/38082/ http://ir.unimas.my/id/eprint/38082/1/Farah%20Wahida%20Binti%20Mamat%20-%2024%20pgs.pdf text en public http://ir.unimas.my/id/eprint/38082/4/Farah%20Wahida.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology Tun Openg Research Fund (F07/TOC/1744/2018)
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English English
topic	Q Science (General) QD Chemistry TP Chemical technology
spellingShingle	Q Science (General) QD Chemistry TP Chemical technology Farah Wahida, Mamat Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris
description	Laccases are multipurpose enzymes that have wide biotechnological applications. The present study seeks to evaluate the feasibility of spent fermentation broth generated upon the bioethanol production from sago hampas, as a feedstock for production of laccases in recombinant Pichia pastoris GS115. Characterisation of the sago bioethanol liquid waste (SBLW) indicated glycerol as the main component, along with glucose and lactic acid. Evaluation of SBLW as a fermentation feedstock for laccase production in P. pastoris GS115 showed that the cell growth was generally feasible when SBLW was used as a feedstock. The activity of laccases reached the highest (0.00076 U mL-1 ± 3.5 × 10-5) in fermentations that employed 40% (v/v) SBLW. This represented 73% of that obtained using the standard synthetic medium. Supplementation of 40% (v/v) SBLW with 1.0% (w/v) yeast extract (YE) yielded enhancements of 1.2-fold and 1.5-fold of biomass concentration and laccase activity, respectively. The expression of laccases was further enhanced when the 40% (v/v) SBLW and 1.0% (w/v) YE was supplemented with 2.0% (v/v) glycerol. The highest laccase activity recorded was 0.00206 U mL-1 ± 5.8 × 10-5 . Both of the biomass and laccase production were increased by 1.9-fold and 2.1-fold in comparison to that achieved by the standard synthetic medium. The efficacy of laccases produced by the optimised SBLW medium was evaluated in terms of the capability of the enzymes to decolourise Remazol Brilliant Blue R (RBBR) dye. The enzyme showed a decolourisation percentage of 68.6%, which represented 91% of the decolourising capability of laccases produced using standard BMMH medium. This indicates the promising efficacy of laccases produced from SBLW. In summary, this work gives an important insight into exploitation of SBLW for production of value-added products. Moreover, this work contributes to the development of recombinant laccase production using low cost and eco-friendly feedstock.
format	Thesis
qualification_level	Master's degree
author	Farah Wahida, Mamat
author_facet	Farah Wahida, Mamat
author_sort	Farah Wahida, Mamat
title	Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris
title_short	Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris
title_full	Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris
title_fullStr	Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris
title_full_unstemmed	Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris
title_sort	feasibility of bioethanol waste stream for production of laccase in pichia pastoris
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2022
url	http://ir.unimas.my/id/eprint/38082/1/Farah%20Wahida%20Binti%20Mamat%20-%2024%20pgs.pdf http://ir.unimas.my/id/eprint/38082/4/Farah%20Wahida.pdf
_version_	1783728496654155776

Feasibility of Bioethanol Waste Stream for Production of Laccase in Pichia pastoris

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