Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak

Yellow stemborer (YSB) (Lepidoptera: Crambidae: Scirpophaga incertulas) is a monophagous insect pest that threatening the production of rice, a human staple food plant. It is speculated that the bacterial community associated with both rice plants and YSB are involved in the feeding process of YSB o...

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Main Author:	Yin Hui, Cheok
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QL Zoology QR Microbiology S Agriculture (General)
Online Access:	http://ir.unimas.my/id/eprint/39290/8/Thesis%20PhD_Cheok%20Yin%20Hui%20-%20fulltext.pdf
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id	my-unimas-ir.39290
record_format	uketd_dc
spelling	my-unimas-ir.392902024-09-13T02:12:01Z Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak 2022 Yin Hui, Cheok QL Zoology QR Microbiology S Agriculture (General) Yellow stemborer (YSB) (Lepidoptera: Crambidae: Scirpophaga incertulas) is a monophagous insect pest that threatening the production of rice, a human staple food plant. It is speculated that the bacterial community associated with both rice plants and YSB are involved in the feeding process of YSB on rice plants. Sarawak has a wide variety of local rice landraces, which may harbour special bacteria communities that contribute to YSB resistance. There is a need to profile the bacterial community associated with local rice landraces and YSB. However, there is a lack of bacterial profiling for Sarawak local rice landraces as well as YSB. Therefore, 16S rDNA amplicon sequencing technique was used to characterise the bacterial community associated with YSB larvae and three selected Sarawak local rice landraces viz. Bajong, Bario, and Bubok. On top of that, the bacterial diversity and composition were compared between bacterial communities from three rice landraces and two rice plant organs. The involvement of YSB infestation in the changes in bacterial community in rice plants was understudied. Therefore, this study also aimed to investigate the change in the diversity and composition of bacterial communities by comparing the bacterial community from YSB free and YSB infested samples. The bacterial community of both YSB and rice plants was dominated by bacteria from phylum Proteobacteria. There is no significant difference in the bacterial diversity of bacterial community associated with three rice landraces, nor do they differ in terms of bacterial composition. Determination of bacterial diversity by rice plant organs was only shown by Faith’s phylogenetic diversity, where the leaf blade has a phylogenetically more diverse bacterial community than the rice stem. The bacterial composition of rice plants was predominantly determined by rice plant organs. YSB infestation had a significant effect on the rice plant's bacterial diversity and composition. Generally, bacterial diversity in rice plants decreased posterior to YSB infestation. The bacterial genera shared between YSB and rice plants infested by YSB but absent in YSB free rice plants suggested a possible bacterial transmission from YSB to rice plants. This result may suggest that YSB infestation has affected the bacterial community of rice plants. Universiti Malaysia Sarawak 2022 Thesis http://ir.unimas.my/id/eprint/39290/ http://ir.unimas.my/id/eprint/39290/8/Thesis%20PhD_Cheok%20Yin%20Hui%20-%20fulltext.pdf text en validuser phd doctoral Universiti Malaysia Sarawak Faculty of Resource Science and Technology Fundamental Research Grant Scheme (FRGS)
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QL Zoology QR Microbiology S Agriculture (General)
spellingShingle	QL Zoology QR Microbiology S Agriculture (General) Yin Hui, Cheok Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak
description	Yellow stemborer (YSB) (Lepidoptera: Crambidae: Scirpophaga incertulas) is a monophagous insect pest that threatening the production of rice, a human staple food plant. It is speculated that the bacterial community associated with both rice plants and YSB are involved in the feeding process of YSB on rice plants. Sarawak has a wide variety of local rice landraces, which may harbour special bacteria communities that contribute to YSB resistance. There is a need to profile the bacterial community associated with local rice landraces and YSB. However, there is a lack of bacterial profiling for Sarawak local rice landraces as well as YSB. Therefore, 16S rDNA amplicon sequencing technique was used to characterise the bacterial community associated with YSB larvae and three selected Sarawak local rice landraces viz. Bajong, Bario, and Bubok. On top of that, the bacterial diversity and composition were compared between bacterial communities from three rice landraces and two rice plant organs. The involvement of YSB infestation in the changes in bacterial community in rice plants was understudied. Therefore, this study also aimed to investigate the change in the diversity and composition of bacterial communities by comparing the bacterial community from YSB free and YSB infested samples. The bacterial community of both YSB and rice plants was dominated by bacteria from phylum Proteobacteria. There is no significant difference in the bacterial diversity of bacterial community associated with three rice landraces, nor do they differ in terms of bacterial composition. Determination of bacterial diversity by rice plant organs was only shown by Faith’s phylogenetic diversity, where the leaf blade has a phylogenetically more diverse bacterial community than the rice stem. The bacterial composition of rice plants was predominantly determined by rice plant organs. YSB infestation had a significant effect on the rice plant's bacterial diversity and composition. Generally, bacterial diversity in rice plants decreased posterior to YSB infestation. The bacterial genera shared between YSB and rice plants infested by YSB but absent in YSB free rice plants suggested a possible bacterial transmission from YSB to rice plants. This result may suggest that YSB infestation has affected the bacterial community of rice plants.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Yin Hui, Cheok
author_facet	Yin Hui, Cheok
author_sort	Yin Hui, Cheok
title	Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak
title_short	Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak
title_full	Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak
title_fullStr	Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak
title_full_unstemmed	Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak
title_sort	relationship between selected rice plant landraces, bacteria and yellow stemborer from sarawak
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology
publishDate	2022
url	http://ir.unimas.my/id/eprint/39290/8/Thesis%20PhD_Cheok%20Yin%20Hui%20-%20fulltext.pdf
_version_	1811771559098974208

Relationship Between Selected Rice Plant Landraces, Bacteria and Yellow Stemborer from Sarawak

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