Cloning and expression of influenza A virus protiens and its use in serological assay

Avian influenza poses a threat to many species including human, as proven by the current situation in Southeast Asia region. It appears that avian influenza of influenza A virus can spread to many species causing severe disease in these new species. The aim of this study is to clone and expressed th...

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Main Author:	Norkasinah, Binti Ramji
Format:	Thesis
Language:	English
Published:	2009
Subjects:	RA0421 Public health Hygiene Preventive Medicine
Online Access:	http://ir.unimas.my/id/eprint/395/2/Norkasinah%20Ramji%20ft.pdf
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id	my-unimas-ir.395
record_format	uketd_dc
spelling	my-unimas-ir.3952024-07-16T01:56:37Z Cloning and expression of influenza A virus protiens and its use in serological assay 2009 Norkasinah, Binti Ramji RA0421 Public health. Hygiene. Preventive Medicine Avian influenza poses a threat to many species including human, as proven by the current situation in Southeast Asia region. It appears that avian influenza of influenza A virus can spread to many species causing severe disease in these new species. The aim of this study is to clone and expressed the major influenza A virus proteins namely matrix 1 (M1), matrix 2 (M2), hemagglutinin 1 (HA1), hemagglutinin 2 (HA2) and nucleoprotein (NP) and also to assess its use in serological assay. Although all genes studied were successfully cloned, only the M1 construct protein resulted in good protein expression. The matrix 1 protein is a conserved protein. The viral gene encoding matrix protein 1 (M1) of influenza A virus is a multifunctional protein that plays essential structural and function roles in virus life cycle. M1 forms the major structural component of the virion, lying beneath the lipid envelope containing the viral hemaglutinin (HA) and neuraminidase (NA) glycoproteins and the M2 ion channel and it is presumed to play an important factor in initiating progeny virus assembly Universiti Malaysia Sarawak, (UNIMAS) 2009 Thesis http://ir.unimas.my/id/eprint/395/ http://ir.unimas.my/id/eprint/395/2/Norkasinah%20Ramji%20ft.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Institute of Health and Community Medicine
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	RA0421 Public health Hygiene Preventive Medicine
spellingShingle	RA0421 Public health Hygiene Preventive Medicine Norkasinah, Binti Ramji Cloning and expression of influenza A virus protiens and its use in serological assay
description	Avian influenza poses a threat to many species including human, as proven by the current situation in Southeast Asia region. It appears that avian influenza of influenza A virus can spread to many species causing severe disease in these new species. The aim of this study is to clone and expressed the major influenza A virus proteins namely matrix 1 (M1), matrix 2 (M2), hemagglutinin 1 (HA1), hemagglutinin 2 (HA2) and nucleoprotein (NP) and also to assess its use in serological assay. Although all genes studied were successfully cloned, only the M1 construct protein resulted in good protein expression. The matrix 1 protein is a conserved protein. The viral gene encoding matrix protein 1 (M1) of influenza A virus is a multifunctional protein that plays essential structural and function roles in virus life cycle. M1 forms the major structural component of the virion, lying beneath the lipid envelope containing the viral hemaglutinin (HA) and neuraminidase (NA) glycoproteins and the M2 ion channel and it is presumed to play an important factor in initiating progeny virus assembly
format	Thesis
qualification_level	Master's degree
author	Norkasinah, Binti Ramji
author_facet	Norkasinah, Binti Ramji
author_sort	Norkasinah, Binti Ramji
title	Cloning and expression of influenza A virus protiens and its use in serological assay
title_short	Cloning and expression of influenza A virus protiens and its use in serological assay
title_full	Cloning and expression of influenza A virus protiens and its use in serological assay
title_fullStr	Cloning and expression of influenza A virus protiens and its use in serological assay
title_full_unstemmed	Cloning and expression of influenza A virus protiens and its use in serological assay
title_sort	cloning and expression of influenza a virus protiens and its use in serological assay
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Institute of Health and Community Medicine
publishDate	2009
url	http://ir.unimas.my/id/eprint/395/2/Norkasinah%20Ramji%20ft.pdf
_version_	1804888406043394048

Cloning and expression of influenza A virus protiens and its use in serological assay

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