In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract

Lipogenesis is a mechanism that enhance lipid deposits in meat, and it can increase the market value of meat as increasing of its content in meat would improve meat quality. The fruit of Shorea macrophylla (engkabang), which is the illipe nut is rich in lipid contents. As the common feed of wild ani...

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Main Author:	Ivy Yee Yen, Chew
Format:	Thesis
Language:	English English English
Published:	2023
Subjects:	Q Science (General) QH301 Biology
Online Access:	http://ir.unimas.my/id/eprint/43057/3/Ivy%20Chew%20Yee%20Yen_dsva.pdf http://ir.unimas.my/id/eprint/43057/4/Master%20Thesis_Ivy%20Chew%20Yee%20Yen%20-%2024%20pages.pdf http://ir.unimas.my/id/eprint/43057/5/Master%20Thesis_Ivy%20Chew%20Yee%20Yen.ftext.pdf
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spelling	my-unimas-ir.430572024-01-16T08:29:47Z In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract 2023-10-06 Ivy Yee Yen, Chew Q Science (General) QH301 Biology Lipogenesis is a mechanism that enhance lipid deposits in meat, and it can increase the market value of meat as increasing of its content in meat would improve meat quality. The fruit of Shorea macrophylla (engkabang), which is the illipe nut is rich in lipid contents. As the common feed of wild animal, its’ composition is then an interested topic to be analysed. To identify if there are any lipogenesis-inducing factors in the engkabang fruit, phytochemical extraction is then carried out using various types of solvents. The isolated compound then was identified using GCMS approach and molecular docking between the lipogenesis-related proteins and identified compounds was carried out. In-vitro analysis was also carried out by treating the crude extracts on 3T3-L1 cells and MTT assay and adipogenesis assay was tested. Ethanol which has the highest polarity have the lowest extraction efficiency at 8.80% while acetone has the highest extraction efficiency at 38.30%. From the GCMS analysis of all the extracts, methyl stearate, methyl elaidate, methyl palmitate and methyl arachidate was found to be available in all the extracts. Methyl stearate is found to be the most abundant in most of the extracts except for acetone. Besides, the molecular docking results shown the four compounds achieved good docking score ranging from -6.7 kcal/mol to -7.1 kcal/mol with SREBP protein. By also considering on amino acid residues interaction, methyl elaidate is suggested to be the most potent lipogenesis-inducing factors which induce lipogenesis via regulation of SREBP protein. The suggested potent lipogenesis-inducing factors then can be included in diet of vertebrates hoping to increase the amount of fat stored in meat and improving its overall quality. University Malaysia Sarawak (UNIMAS) 2023-10 Thesis http://ir.unimas.my/id/eprint/43057/ http://ir.unimas.my/id/eprint/43057/3/Ivy%20Chew%20Yee%20Yen_dsva.pdf text en staffonly http://ir.unimas.my/id/eprint/43057/4/Master%20Thesis_Ivy%20Chew%20Yee%20Yen%20-%2024%20pages.pdf text en public http://ir.unimas.my/id/eprint/43057/5/Master%20Thesis_Ivy%20Chew%20Yee%20Yen.ftext.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Resource Science and Technology
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English English English
topic	Q Science (General) QH301 Biology
spellingShingle	Q Science (General) QH301 Biology Ivy Yee Yen, Chew In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract
description	Lipogenesis is a mechanism that enhance lipid deposits in meat, and it can increase the market value of meat as increasing of its content in meat would improve meat quality. The fruit of Shorea macrophylla (engkabang), which is the illipe nut is rich in lipid contents. As the common feed of wild animal, its’ composition is then an interested topic to be analysed. To identify if there are any lipogenesis-inducing factors in the engkabang fruit, phytochemical extraction is then carried out using various types of solvents. The isolated compound then was identified using GCMS approach and molecular docking between the lipogenesis-related proteins and identified compounds was carried out. In-vitro analysis was also carried out by treating the crude extracts on 3T3-L1 cells and MTT assay and adipogenesis assay was tested. Ethanol which has the highest polarity have the lowest extraction efficiency at 8.80% while acetone has the highest extraction efficiency at 38.30%. From the GCMS analysis of all the extracts, methyl stearate, methyl elaidate, methyl palmitate and methyl arachidate was found to be available in all the extracts. Methyl stearate is found to be the most abundant in most of the extracts except for acetone. Besides, the molecular docking results shown the four compounds achieved good docking score ranging from -6.7 kcal/mol to -7.1 kcal/mol with SREBP protein. By also considering on amino acid residues interaction, methyl elaidate is suggested to be the most potent lipogenesis-inducing factors which induce lipogenesis via regulation of SREBP protein. The suggested potent lipogenesis-inducing factors then can be included in diet of vertebrates hoping to increase the amount of fat stored in meat and improving its overall quality.
format	Thesis
qualification_level	Master's degree
author	Ivy Yee Yen, Chew
author_facet	Ivy Yee Yen, Chew
author_sort	Ivy Yee Yen, Chew
title	In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract
title_short	In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract
title_full	In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract
title_fullStr	In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract
title_full_unstemmed	In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract
title_sort	in-silico and in-vitro analysis of lipogenesis effect of shorea macrophylla fruits’ crude extract
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Resource Science and Technology
publishDate	2023
url	http://ir.unimas.my/id/eprint/43057/3/Ivy%20Chew%20Yee%20Yen_dsva.pdf http://ir.unimas.my/id/eprint/43057/4/Master%20Thesis_Ivy%20Chew%20Yee%20Yen%20-%2024%20pages.pdf http://ir.unimas.my/id/eprint/43057/5/Master%20Thesis_Ivy%20Chew%20Yee%20Yen.ftext.pdf
_version_	1794023042520711168

In-silico and in-vitro analysis of Lipogenesis Effect of Shorea macrophylla Fruits’ Crude Extract

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