Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)

The unregulated and/or incorrect use of antimicrobial agents against diseases of farmed aquatic species poses a considerable threat to the development and growth of a sustainable aquaculture industry. Hence, vaccination has emerged as a pivotal strategy to mitigate this threat. This study marks the...

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Main Author:	CINDY JIA YUNG, KHO
Format:	Thesis
Language:	English English English
Published:	2024
Subjects:	QR180 Immunology
Online Access:	http://ir.unimas.my/id/eprint/44661/3/Thesis%20Master_Cindy%20Kho%20Jia%20Yung.ftext.pdf http://ir.unimas.my/id/eprint/44661/4/Thesis%20Master_Cindy%20Kho%20Jia%20Yung%20-24%20pages.pdf http://ir.unimas.my/id/eprint/44661/5/DSVA%20_Cindy%20Kho%20Jia.pdf
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id	my-unimas-ir.44661
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spelling	my-unimas-ir.446612024-05-10T02:35:55Z Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau) 2024-05-02 CINDY JIA YUNG, KHO QR180 Immunology The unregulated and/or incorrect use of antimicrobial agents against diseases of farmed aquatic species poses a considerable threat to the development and growth of a sustainable aquaculture industry. Hence, vaccination has emerged as a pivotal strategy to mitigate this threat. This study marks the first utilization of reverse vaccinology to develop subunit vaccines against Pseudomonas koreensis infection in Empurau (Tor tambroides). WGS analysis revealed that the genomic size of P. koreensis CM-01 is 6,171,880 bp and has a G+C content of 60.5%. The proteome (5,538 proteins) was screened against various filters to prioritize proteins based on features that are associated with virulence, subcellular localization, transmembrane helical structure, antigenicity, essentiality, non-homology with the host proteome, molecular weight, and stability, which led to the identification of eight potential vaccine candidates. These potential vaccine candidates were cloned and expressed, with six achieving successful expression and purification. The antigens were formulated into two distinct vaccine mixtures, Vac A and Vac B, and their protective efficacy was assessed through in vivo challenge experiments. Vac A and Vac B demonstrated high protective efficacies of 100% and 81.2%, respectively. Histological analyses revealed reduced tissue damage in vaccinated fish after experimental infection, with Vac A showing no adverse effects, whereas Vac B exhibited mild degenerative changes. Quantitative real-time PCR results showed a significant upregulation of TNF-α and downregulation of IL-1β in the kidneys, spleen, gills, and intestine in both Vac A- and Vac B immunized fish after challenged with P. koreensis. Additionally, IL-8 exhibits tissue-specific differential expression, with significant upregulation in the kidney, gills, and intestine, and downregulation in the spleen, particularly notable in Vac A immunized fish. The research underscores the effectiveness of the reverse vaccinology approach in fish and demonstrates the promising potential of Vac A and Vac B as recombinant subunit vaccines. Not applicable 2024-05 Thesis http://ir.unimas.my/id/eprint/44661/ http://ir.unimas.my/id/eprint/44661/3/Thesis%20Master_Cindy%20Kho%20Jia%20Yung.ftext.pdf text en validuser http://ir.unimas.my/id/eprint/44661/4/Thesis%20Master_Cindy%20Kho%20Jia%20Yung%20-24%20pages.pdf text en public http://ir.unimas.my/id/eprint/44661/5/DSVA%20_Cindy%20Kho%20Jia.pdf text en staffonly masters UNIMAS FACULTY OF RESOURCES SCIENCE AND TECHNOLOGY
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English English English
topic	QR180 Immunology
spellingShingle	QR180 Immunology CINDY JIA YUNG, KHO Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)
description	The unregulated and/or incorrect use of antimicrobial agents against diseases of farmed aquatic species poses a considerable threat to the development and growth of a sustainable aquaculture industry. Hence, vaccination has emerged as a pivotal strategy to mitigate this threat. This study marks the first utilization of reverse vaccinology to develop subunit vaccines against Pseudomonas koreensis infection in Empurau (Tor tambroides). WGS analysis revealed that the genomic size of P. koreensis CM-01 is 6,171,880 bp and has a G+C content of 60.5%. The proteome (5,538 proteins) was screened against various filters to prioritize proteins based on features that are associated with virulence, subcellular localization, transmembrane helical structure, antigenicity, essentiality, non-homology with the host proteome, molecular weight, and stability, which led to the identification of eight potential vaccine candidates. These potential vaccine candidates were cloned and expressed, with six achieving successful expression and purification. The antigens were formulated into two distinct vaccine mixtures, Vac A and Vac B, and their protective efficacy was assessed through in vivo challenge experiments. Vac A and Vac B demonstrated high protective efficacies of 100% and 81.2%, respectively. Histological analyses revealed reduced tissue damage in vaccinated fish after experimental infection, with Vac A showing no adverse effects, whereas Vac B exhibited mild degenerative changes. Quantitative real-time PCR results showed a significant upregulation of TNF-α and downregulation of IL-1β in the kidneys, spleen, gills, and intestine in both Vac A- and Vac B immunized fish after challenged with P. koreensis. Additionally, IL-8 exhibits tissue-specific differential expression, with significant upregulation in the kidney, gills, and intestine, and downregulation in the spleen, particularly notable in Vac A immunized fish. The research underscores the effectiveness of the reverse vaccinology approach in fish and demonstrates the promising potential of Vac A and Vac B as recombinant subunit vaccines.
format	Thesis
qualification_level	Master's degree
author	CINDY JIA YUNG, KHO
author_facet	CINDY JIA YUNG, KHO
author_sort	CINDY JIA YUNG, KHO
title	Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)
title_short	Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)
title_full	Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)
title_fullStr	Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)
title_full_unstemmed	Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)
title_sort	selection of vaccine candidates against pseudomonas koreensis using reverse vaccinology and a preliminary efficacy trial in tor tambroides (empurau)
granting_institution	UNIMAS
granting_department	FACULTY OF RESOURCES SCIENCE AND TECHNOLOGY
publishDate	2024
url	http://ir.unimas.my/id/eprint/44661/3/Thesis%20Master_Cindy%20Kho%20Jia%20Yung.ftext.pdf http://ir.unimas.my/id/eprint/44661/4/Thesis%20Master_Cindy%20Kho%20Jia%20Yung%20-24%20pages.pdf http://ir.unimas.my/id/eprint/44661/5/DSVA%20_Cindy%20Kho%20Jia.pdf
_version_	1804888431823683584

Selection of Vaccine Candidates Against Pseudomonas koreensis Using Reverse Vaccinology and a Preliminary Efficacy Trial in Tor tambroides (Empurau)

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