Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak

The objectives of this study were to develop species specific polymerase chain reaction (PCR) primers for molecular detection of monkey malaria parasites and to use molecular methods to identify and characterize plasmodium species from macaques. Since P. knowlesi is potentially of zoonotic origin, t...

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Main Author:	Divis, Paul Cliff Simon
Format:	Thesis
Language:	English
Published:	2007
Subjects:	QR180 Immunology
Online Access:	http://ir.unimas.my/id/eprint/601/1/Paul%20Cliff%20Simon%20Divis.pdf
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id	my-unimas-ir.601
record_format	uketd_dc
spelling	my-unimas-ir.6012023-05-11T09:28:10Z Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak 2007 Divis, Paul Cliff Simon QR180 Immunology The objectives of this study were to develop species specific polymerase chain reaction (PCR) primers for molecular detection of monkey malaria parasites and to use molecular methods to identify and characterize plasmodium species from macaques. Since P. knowlesi is potentially of zoonotic origin, these methods would assist in determining whether macaques are the natural reservoir of P. knowlesi in the Kapit Division. In this study, species-specific PCR primers for the identification of P. knowlesi, P. cynomolgi, P. coatneyi, P. fieldi, P. inui and P. fragile were developed based on the variable regions in the small subunit ribosomal RNA (SSU rRNA) genes. Universiti Malaysia Sarawak, (UNIMAS) 2007 Thesis http://ir.unimas.my/id/eprint/601/ http://ir.unimas.my/id/eprint/601/1/Paul%20Cliff%20Simon%20Divis.pdf text en validuser masters Universiti Malaysia Sarawak (UNIMAS) Faculty of Medicine and Health Sciences
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QR180 Immunology
spellingShingle	QR180 Immunology Divis, Paul Cliff Simon Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak
description	The objectives of this study were to develop species specific polymerase chain reaction (PCR) primers for molecular detection of monkey malaria parasites and to use molecular methods to identify and characterize plasmodium species from macaques. Since P. knowlesi is potentially of zoonotic origin, these methods would assist in determining whether macaques are the natural reservoir of P. knowlesi in the Kapit Division. In this study, species-specific PCR primers for the identification of P. knowlesi, P. cynomolgi, P. coatneyi, P. fieldi, P. inui and P. fragile were developed based on the variable regions in the small subunit ribosomal RNA (SSU rRNA) genes.
format	Thesis
qualification_level	Master's degree
author	Divis, Paul Cliff Simon
author_facet	Divis, Paul Cliff Simon
author_sort	Divis, Paul Cliff Simon
title	Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak
title_short	Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak
title_full	Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak
title_fullStr	Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak
title_full_unstemmed	Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak
title_sort	identification and molecular characterisation of malaria parasites of macaques in kapit, sarawak
granting_institution	Universiti Malaysia Sarawak (UNIMAS)
granting_department	Faculty of Medicine and Health Sciences
publishDate	2007
url	http://ir.unimas.my/id/eprint/601/1/Paul%20Cliff%20Simon%20Divis.pdf
_version_	1783727894563913728

Identification and molecular characterisation of malaria parasites of macaques in Kapit, Sarawak

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