Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw

Chemical studies and biological activities were performed on Goniothalamus tapis Miq and G. velutinus Airy Shaw. Methanolic extracts were partitioned using solvents with increasing polarity to give hexane, dichloromethane, ethyl acetate and methanol partitions. Purification through extensive column...

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Main Author:	Nabihah, Hamdan
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/8348/1/Nabihah%20Hamdan%20ft.pdf
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spelling	my-unimas-ir.83482023-05-24T03:00:03Z Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw 2014 Nabihah, Hamdan QR Microbiology Chemical studies and biological activities were performed on Goniothalamus tapis Miq and G. velutinus Airy Shaw. Methanolic extracts were partitioned using solvents with increasing polarity to give hexane, dichloromethane, ethyl acetate and methanol partitions. Purification through extensive column chromatography, preparative thin layer chromatography and recrystallization resulted in the isolation and characterization of 5-hydroxy-3-amino-2-aceto- 1,4-napthoquinone and 9-deoxygoniopypyrone from G. tapis, while goniothalamin, aristolactam AII, velutinam and 5,7-dihydroxyflavone were characterized from G. velutinus. Structures of all pure compounds were elucidated based on their spectroscopic data especially mass spectrometry, infrared, nuclear magnetic resonance and comparison with published information. Biological activities screening (antibacterial, antioxidant, brine shrimp toxicity and termiticidal) were performed on all partitions of G. tapis and G. velutinus (bark and root). All G. tapis partitions showed weak antibacterial activities against Bacillus megaterium, Lysteria monocytogenes, Escherichia coli and Salmonella entrica with inhibition zone in the range of 7.2 to 9.7 mm. Ethyl acetate (GTEt) and methanol (GTM) partitions of G. tapis showed strong ability to scavenge DPPH free radicals with EC50 74.5 and 86.0 ppm, respectively and comparable to positive control, butylated hydroxyl toluene with EC50 of 110.1 ppm. 9-Deoxygoniopypyrone, goniothalamin and 5,7-dihydroxyflavone showed weak antioxidant activity with EC50 more than 150.0 ppm. Dichloromethane partitions of G. tapis and G. velutinus barks (GTD and GVBD) exhibited strong toxicity against brine shrimp, Artemia salina with LC50 of 38.2 and 45.1 ppm, respectively and comparable with thymol which was used as positive control with LC50 of 37.5 ppm. Ethyl acetate partition of G. tapis (GTEt) and dichloromethane partition of G. velutinus (GVBD) showed potent termiticidal activity against Coptotermes sp. with 100% mortality at concentration of 10% on the third day of observation. GTEt and GVBD partitions exhibited strongest termiticidal activities with LC50 of 0.04 and 0.06%, respectively. Universiti Malaysia Sarawak, (UNIMAS) 2014 Thesis http://ir.unimas.my/id/eprint/8348/ http://ir.unimas.my/id/eprint/8348/1/Nabihah%20Hamdan%20ft.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology.
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Nabihah, Hamdan Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw
description	Chemical studies and biological activities were performed on Goniothalamus tapis Miq and G. velutinus Airy Shaw. Methanolic extracts were partitioned using solvents with increasing polarity to give hexane, dichloromethane, ethyl acetate and methanol partitions. Purification through extensive column chromatography, preparative thin layer chromatography and recrystallization resulted in the isolation and characterization of 5-hydroxy-3-amino-2-aceto- 1,4-napthoquinone and 9-deoxygoniopypyrone from G. tapis, while goniothalamin, aristolactam AII, velutinam and 5,7-dihydroxyflavone were characterized from G. velutinus. Structures of all pure compounds were elucidated based on their spectroscopic data especially mass spectrometry, infrared, nuclear magnetic resonance and comparison with published information. Biological activities screening (antibacterial, antioxidant, brine shrimp toxicity and termiticidal) were performed on all partitions of G. tapis and G. velutinus (bark and root). All G. tapis partitions showed weak antibacterial activities against Bacillus megaterium, Lysteria monocytogenes, Escherichia coli and Salmonella entrica with inhibition zone in the range of 7.2 to 9.7 mm. Ethyl acetate (GTEt) and methanol (GTM) partitions of G. tapis showed strong ability to scavenge DPPH free radicals with EC50 74.5 and 86.0 ppm, respectively and comparable to positive control, butylated hydroxyl toluene with EC50 of 110.1 ppm. 9-Deoxygoniopypyrone, goniothalamin and 5,7-dihydroxyflavone showed weak antioxidant activity with EC50 more than 150.0 ppm. Dichloromethane partitions of G. tapis and G. velutinus barks (GTD and GVBD) exhibited strong toxicity against brine shrimp, Artemia salina with LC50 of 38.2 and 45.1 ppm, respectively and comparable with thymol which was used as positive control with LC50 of 37.5 ppm. Ethyl acetate partition of G. tapis (GTEt) and dichloromethane partition of G. velutinus (GVBD) showed potent termiticidal activity against Coptotermes sp. with 100% mortality at concentration of 10% on the third day of observation. GTEt and GVBD partitions exhibited strongest termiticidal activities with LC50 of 0.04 and 0.06%, respectively.
format	Thesis
qualification_level	Master's degree
author	Nabihah, Hamdan
author_facet	Nabihah, Hamdan
author_sort	Nabihah, Hamdan
title	Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw
title_short	Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw
title_full	Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw
title_fullStr	Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw
title_full_unstemmed	Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw
title_sort	chemical studies and biological activities of goniothalamus tapis miq and goniothalamus velutinus airy shaw
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology.
publishDate	2014
url	http://ir.unimas.my/id/eprint/8348/1/Nabihah%20Hamdan%20ft.pdf
_version_	1783727985738645504

Chemical studies and biological activities of Goniothalamus Tapis Miq and Goniothalamus Velutinus Airy Shaw

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