Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia

The emergence and re-emergence or antimicrobial resistant diseases are one of the health threatening phenomenons and become the main causative for significant mortality and morbidity in human worldwide. Fungi, on the other hand, are a renowned kingdom as important source of pharmaceutically bioactiv...

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Main Author:	Anita, Tahir
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QR Microbiology
Online Access:	http://ir.unimas.my/id/eprint/8352/1/Anita%20Binti%20Tahir%20ft.pdf
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id	my-unimas-ir.8352
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spelling	my-unimas-ir.83522023-03-24T04:27:52Z Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia 2014 Anita, Tahir QR Microbiology The emergence and re-emergence or antimicrobial resistant diseases are one of the health threatening phenomenons and become the main causative for significant mortality and morbidity in human worldwide. Fungi, on the other hand, are a renowned kingdom as important source of pharmaceutically bioactive compounds thus offers an option for controlling the spread of antimicrobial resistant pathogens. The present study aims to isolate and identify compound(s) with antimicrobial properties from fungi isolated endophytic-, marine- and soil-origin. Bioactive compounds from selected fungi cultivated in Potato Dextrose agar, Czapek Dox agar and V8 Juice agar, were extracted using hexane, dichloromethane, ethyl acetate, methanol and water solvent. The extracts bioactivities against Staphylococcus aureus, Salmonella typhi, Escherichia coli and Enterobacter aerogenes were assessed via series of bioassay-guided approach that employed diffusion, dilution and bioautography assay coupled with thin layer chromatography method. The cytotoxicity of semipure fractions were tested and the bioactive compounds were identified using gas chromatography-mass spectrometry (GC-MS). The studied fungi were identified using phenotypic and genotypic methods. In preliminary study, hexane extracts of fungi collected from three types media were found active against at least two bacteria, S. typhi and E. aerogenes. However, V8JA produced higher amount hexane thus become the main media for production of other fungi. After being screened through a series of bioassay-guided method, two hexane subfractions from fungi isolates 1C and L10.1.F3, denoted as H.1C.1.4 and H.L10.1.4, were found potentially yielding bioactive compounds. The LC50 of subfraction H.1C.1.4 was approximately 100 µg/ml while no toxicity effect was detected to Vero cells for 2 subfraction H.L10.1.4 at the same concentration. Four compounds were identified via GC-MS, two inclusive for both fractions namely 1-Nonadecanol, 3-Hexen-2-one while 1-Pentadecanol and 1-Heptacosanol were exclusive to semipure fraction H.1C.1.1.4 and H.L10.1.1.4, respectively. Using 16S rDNA partial sequence analysis, fungi 1C and L10.1.F3 were identified as Penicillium sclerotiorum. Additionally, fungi isolate AF1.4 is Penicillium simplicissimum while fungi isolates AF2.1, L12.1F1 and 2D were identified as Penicillium dipodomyicola. The present study highlights the potential of indigenous microbe in Sarawak, Malaysia as source of biologically active compounds for pharmacology. Universiti Malaysia Sarawak, (UNIMAS) 2014 Thesis http://ir.unimas.my/id/eprint/8352/ http://ir.unimas.my/id/eprint/8352/1/Anita%20Binti%20Tahir%20ft.pdf text en validuser masters Universiti Malaysia Sarawak Faculty of Resource Science and Technology.
institution	Universiti Malaysia Sarawak
collection	UNIMAS Institutional Repository
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Anita, Tahir Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia
description	The emergence and re-emergence or antimicrobial resistant diseases are one of the health threatening phenomenons and become the main causative for significant mortality and morbidity in human worldwide. Fungi, on the other hand, are a renowned kingdom as important source of pharmaceutically bioactive compounds thus offers an option for controlling the spread of antimicrobial resistant pathogens. The present study aims to isolate and identify compound(s) with antimicrobial properties from fungi isolated endophytic-, marine- and soil-origin. Bioactive compounds from selected fungi cultivated in Potato Dextrose agar, Czapek Dox agar and V8 Juice agar, were extracted using hexane, dichloromethane, ethyl acetate, methanol and water solvent. The extracts bioactivities against Staphylococcus aureus, Salmonella typhi, Escherichia coli and Enterobacter aerogenes were assessed via series of bioassay-guided approach that employed diffusion, dilution and bioautography assay coupled with thin layer chromatography method. The cytotoxicity of semipure fractions were tested and the bioactive compounds were identified using gas chromatography-mass spectrometry (GC-MS). The studied fungi were identified using phenotypic and genotypic methods. In preliminary study, hexane extracts of fungi collected from three types media were found active against at least two bacteria, S. typhi and E. aerogenes. However, V8JA produced higher amount hexane thus become the main media for production of other fungi. After being screened through a series of bioassay-guided method, two hexane subfractions from fungi isolates 1C and L10.1.F3, denoted as H.1C.1.4 and H.L10.1.4, were found potentially yielding bioactive compounds. The LC50 of subfraction H.1C.1.4 was approximately 100 µg/ml while no toxicity effect was detected to Vero cells for 2 subfraction H.L10.1.4 at the same concentration. Four compounds were identified via GC-MS, two inclusive for both fractions namely 1-Nonadecanol, 3-Hexen-2-one while 1-Pentadecanol and 1-Heptacosanol were exclusive to semipure fraction H.1C.1.1.4 and H.L10.1.1.4, respectively. Using 16S rDNA partial sequence analysis, fungi 1C and L10.1.F3 were identified as Penicillium sclerotiorum. Additionally, fungi isolate AF1.4 is Penicillium simplicissimum while fungi isolates AF2.1, L12.1F1 and 2D were identified as Penicillium dipodomyicola. The present study highlights the potential of indigenous microbe in Sarawak, Malaysia as source of biologically active compounds for pharmacology.
format	Thesis
qualification_level	Master's degree
author	Anita, Tahir
author_facet	Anita, Tahir
author_sort	Anita, Tahir
title	Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia
title_short	Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia
title_full	Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia
title_fullStr	Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia
title_full_unstemmed	Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia
title_sort	isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in sarawak, malaysia
granting_institution	Universiti Malaysia Sarawak
granting_department	Faculty of Resource Science and Technology.
publishDate	2014
url	http://ir.unimas.my/id/eprint/8352/1/Anita%20Binti%20Tahir%20ft.pdf
_version_	1783727986856427520

Isolation and screening of potential antimicrobial metabolites from selected endophytic, marine and soil fungi in Sarawak, Malaysia

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