Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases.
Nasopharyngeal carcinoma (NPC) is a cancer of the head and neck that is highly associated with Epstein - Barr virus (EBV) infection and shows strong ethnic and geographical clustering. In Malaysia, NPC is the fourth most common cancer on overall and the third most common cancer among males. The d...
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my-unimas-ir.93522023-03-28T03:48:58Z Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. 2015 Ma, Xiang Ru Q Science (General) R Medicine (General) Nasopharyngeal carcinoma (NPC) is a cancer of the head and neck that is highly associated with Epstein - Barr virus (EBV) infection and shows strong ethnic and geographical clustering. In Malaysia, NPC is the fourth most common cancer on overall and the third most common cancer among males. The disease is often diagnosed at relatively later stages due to the signs and symptoms that are not obvious and which could often be mistaken as common illness. It is thus important to identify the molecular pathway(s) and genes involved in NPC carcinogenesis in order to have better prognosis of the disease. Ribosomal protein (RP) genes have recently been implicated in many human disorders and diseases. Apart from their roles in the canonical protein biosynthesis pathway, studies have shown that RP genes could also have extra ribosomal functions. Herein, the potential involvements and role(s) of nine selected ribosomal protein (RP) genes were examined at transcript level in NPC-derived cell lines and paired biopsies using real-time PCR, microarray and DNA sequencing techniques. Westernblotting was performed on NPC-derived cell lines to study the expression of RPs at protein level. Student’s- t test, correlation test and multiple linear regression test were used to determine the statistical significance of result obtained. Both RPS15 and L14 were underexpressed at transcript level in cases of NPC whereas RPS3, S7, S15, S26, S27, L32 and L34 were not differentially expressed. Protein-protein dock models built via bioinformatics approach showed potential interactions between RPS15 with the Agenet-like motif 1 of FMRP. This motif is located in the NDF domain that has been reported to be involved in protein-RNA and protein-protein interactions. No nucleotide aberrancy was detected in the coding regions of all nine RPs examined. There was no association established between the expression of each RP with p53, as well as with NPC related clinicopathologic factors studied.p53 which normally acts as the genome guardian of cells was also not differentially expressed at transcript and protein levels; and no mutation was detected in its entire coding region. Current findings suggested possible involvement of RPS15 and L14 in NPC pathogenesis. RPS15 protein could possibly regulate translation by interacting with FMRP – a predicted function that warrants further experimental investigation. Evidence showed that RPS3, S7, S15, S26, S27, L32 and L34 were unlikely to be directly involved in NPC pathogenesis. All these further strengthen the view that NPC is a unique and distinct type of head and neck cancers. Universiti Malaysia Sarawak, (UNIMAS) 2015 Thesis http://ir.unimas.my/id/eprint/9352/ http://ir.unimas.my/id/eprint/9352/3/Molecular%20Genetic%20Study%20of%20Selected%20Ribosomal%20Protein%20Genes%20In%20Nasopharyngeal%20Carcinoma%20Cases.pdf text en validuser phd doctoral Universiti Malaysia Sarawak, (UNIMAS) Faculty of Resource Science and Technology. |
| institution |
Universiti Malaysia Sarawak |
| collection |
UNIMAS Institutional Repository |
| language |
English |
| topic |
Q Science (General) R Medicine (General) |
| spellingShingle |
Q Science (General) R Medicine (General) Ma, Xiang Ru Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. |
| description |
Nasopharyngeal carcinoma (NPC) is a cancer of the head and neck that is highly associated
with Epstein - Barr virus (EBV) infection and shows strong ethnic and geographical
clustering. In Malaysia, NPC is the fourth most common cancer on overall and the third most
common cancer among males. The disease is often diagnosed at relatively later stages due to
the signs and symptoms that are not obvious and which could often be mistaken as common
illness. It is thus important to identify the molecular pathway(s) and genes involved in NPC
carcinogenesis in order to have better prognosis of the disease. Ribosomal protein (RP) genes
have recently been implicated in many human disorders and diseases. Apart from their roles
in the canonical protein biosynthesis pathway, studies have shown that RP genes could also
have extra ribosomal functions. Herein, the potential involvements and role(s) of nine selected
ribosomal protein (RP) genes were examined at transcript level in NPC-derived cell lines and
paired biopsies using real-time PCR, microarray and DNA sequencing techniques. Westernblotting
was performed on NPC-derived cell lines to study the expression of RPs at protein
level. Student’s- t test, correlation test and multiple linear regression test were used to
determine the statistical significance of result obtained. Both RPS15 and L14 were
underexpressed at transcript level in cases of NPC whereas RPS3, S7, S15, S26, S27, L32 and
L34 were not differentially expressed. Protein-protein dock models built via bioinformatics
approach showed potential interactions between RPS15 with the Agenet-like motif 1 of
FMRP. This motif is located in the NDF domain that has been reported to be involved in
protein-RNA and protein-protein interactions. No nucleotide aberrancy was detected in the
coding regions of all nine RPs examined. There was no association established between the
expression of each RP with p53, as well as with NPC related clinicopathologic factors studied.p53 which normally acts as the genome guardian of cells was also not differentially expressed
at transcript and protein levels; and no mutation was detected in its entire coding region.
Current findings suggested possible involvement of RPS15 and L14 in NPC pathogenesis.
RPS15 protein could possibly regulate translation by interacting with FMRP – a predicted
function that warrants further experimental investigation. Evidence showed that RPS3, S7,
S15, S26, S27, L32 and L34 were unlikely to be directly involved in NPC pathogenesis. All
these further strengthen the view that NPC is a unique and distinct type of head and neck
cancers. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Ma, Xiang Ru |
| author_facet |
Ma, Xiang Ru |
| author_sort |
Ma, Xiang Ru |
| title |
Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. |
| title_short |
Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. |
| title_full |
Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. |
| title_fullStr |
Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. |
| title_full_unstemmed |
Molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. |
| title_sort |
molecular genetic study of selected ribosomal protein genes in nasopharyngeal carcinoma cases. |
| granting_institution |
Universiti Malaysia Sarawak, (UNIMAS) |
| granting_department |
Faculty of Resource Science and Technology. |
| publishDate |
2015 |
| url |
http://ir.unimas.my/id/eprint/9352/3/Molecular%20Genetic%20Study%20of%20Selected%20Ribosomal%20Protein%20Genes%20In%20Nasopharyngeal%20Carcinoma%20Cases.pdf |
| _version_ |
1783728051363774464 |