Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)

Long term storage of recalcitrant seeds by conventional storage methods have by far been unsuccessful. In this study the excised embryos of seeds of jackfruit (Artocarpus heterophyllus). a recalcitrant species has been attempted at for long term preservation using cryogenic means. Stage of fruit...

Full description

Saved in:
Bibliographic Details
Main Author: Krishnapillay, Baskaran
Format: Thesis
Language:English
English
Published: 1989
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/10269/1/FP_1989_8_A.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-upm-ir.10269
record_format uketd_dc
spelling my-upm-ir.102692011-03-21T02:17:46Z Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.) 1989 Krishnapillay, Baskaran Long term storage of recalcitrant seeds by conventional storage methods have by far been unsuccessful. In this study the excised embryos of seeds of jackfruit (Artocarpus heterophyllus). a recalcitrant species has been attempted at for long term preservation using cryogenic means. Stage of fruit maturity was critical in the study where embryos excised from mature unripe fruits were found to be the most appropriate. Selection of uniform sized embryos (4.0 - 5.0 m m in length) minimised variability in embryo fresh weight and moisture content to a considerable extent. A modified Murashige and Skoog medium containing 1.0 mg/l each of NAA and BAP, 2 gil of activated charcoal and 170 mg/l of NaH2 P04. H20 was found to be a suitable medium for development and growth of the excised jackfruit embryos. The safe critical moisture limit for excised jackfruit embryos was found to be around 14-15%. Storage of embryos dried to this moisture limit by conventional storage methods with a view to long term storage was unsuccessful. The maximum storage period achieved was only one month. Attempts at direct storage in liquid nitrogen after desiccation to near critical moisture limit was also found to be unsuccessful. Artocarpus Plant cells and tissues - Cryopreservation 1989 Thesis http://psasir.upm.edu.my/id/eprint/10269/ http://psasir.upm.edu.my/id/eprint/10269/1/FP_1989_8_A.pdf application/pdf en public phd doctoral Universiti Pertanian Malaysia Artocarpus Plant cells and tissues - Cryopreservation Faculty of Agriculture English
institution Universiti Putra Malaysia
collection PSAS Institutional Repository
language English
English
topic Artocarpus
Plant cells and tissues - Cryopreservation

spellingShingle Artocarpus
Plant cells and tissues - Cryopreservation

Krishnapillay, Baskaran
Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
description Long term storage of recalcitrant seeds by conventional storage methods have by far been unsuccessful. In this study the excised embryos of seeds of jackfruit (Artocarpus heterophyllus). a recalcitrant species has been attempted at for long term preservation using cryogenic means. Stage of fruit maturity was critical in the study where embryos excised from mature unripe fruits were found to be the most appropriate. Selection of uniform sized embryos (4.0 - 5.0 m m in length) minimised variability in embryo fresh weight and moisture content to a considerable extent. A modified Murashige and Skoog medium containing 1.0 mg/l each of NAA and BAP, 2 gil of activated charcoal and 170 mg/l of NaH2 P04. H20 was found to be a suitable medium for development and growth of the excised jackfruit embryos. The safe critical moisture limit for excised jackfruit embryos was found to be around 14-15%. Storage of embryos dried to this moisture limit by conventional storage methods with a view to long term storage was unsuccessful. The maximum storage period achieved was only one month. Attempts at direct storage in liquid nitrogen after desiccation to near critical moisture limit was also found to be unsuccessful.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Krishnapillay, Baskaran
author_facet Krishnapillay, Baskaran
author_sort Krishnapillay, Baskaran
title Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_short Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_full Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_fullStr Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_full_unstemmed Towards The Development of a Protocol for Cryopreservation of Embryos of a Recalcitrant Seed (Artocarpus Heterophyllus Lam.)
title_sort towards the development of a protocol for cryopreservation of embryos of a recalcitrant seed (artocarpus heterophyllus lam.)
granting_institution Universiti Pertanian Malaysia
granting_department Faculty of Agriculture
publishDate 1989
url http://psasir.upm.edu.my/id/eprint/10269/1/FP_1989_8_A.pdf
_version_ 1747811084214992896