Etiology and Control of Phytophthora Infestans (Mont.) De Bary in Malaysia
Phytophthora infestans (Mont.) De Bary is the causal agent of late blight disease of tomato, a major destructive disease of tomato in Cameron Highlands. The study undertaken was to isolate the pathogen on artificial media. The morphology and cultural characteristics were described. Disease deve...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1994
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10345/1/FP_1994_4_A.pdf |
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| Summary: | Phytophthora infestans (Mont.) De Bary is the causal
agent of late blight disease of tomato, a major destructive
disease of tomato in Cameron Highlands. The study
undertaken was to isolate the pathogen on artificial media.
The morphology and cultural characteristics were described.
Disease development of P. infestans isolates on tomato was
also studied. In-vivo sensitivity of the isolates towards
metalaxyl was tested. Disease epidemiology studies were
conducted during the two tomato planting periods in Cameron
Highlands.
Three isolates of P. infestans from Cameron High lands
were isolated on Rye Grain Agar (RGrA), V-8 Juice Agar
(V8JA) and Rye wholemeal Agar (RWMA) allamended with
antibiotics with percentage recovery of 2%, 0.1% and
0.4% respectively. The P. infestans isolates were
designated as PIB/01, PIH/01 and PIKR/01. No growth was
observed on the two other media viz. Pea Agar and Difco
LBA. Successful isolation were aided by baiting using
potato tuber slices.
Morphological studies of the P. infestans isolate
showed that the mean zoosporangia size was 37.25um x
19.71um, mean L/B ratio of 1.91 and mean short pedicel
length of 1.60um. Mean zoospores diameter measured 9.99
um. No chlamydospores was produced in culture. |
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