Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia

Malaysia is blessed with rich biodiversity and her people have harnessed this virtue in many ways such as for medicinal uses. However, not all of the plants could be easily grown or harvest for its bioactive natural products for use. Endophytes which are microorganisms that live in plants could b...

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Main Author:	Mohamed Rafi, Mohamed Ikhtifar
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Endophytes
Online Access:	http://psasir.upm.edu.my/id/eprint/103836/1/MOHAMED%20IKHTIFAR%20BIN%20MOHA%20-%20IR.pdf
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spelling	my-upm-ir.1038362023-05-09T08:10:02Z Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia 2018-11 Mohamed Rafi, Mohamed Ikhtifar Malaysia is blessed with rich biodiversity and her people have harnessed this virtue in many ways such as for medicinal uses. However, not all of the plants could be easily grown or harvest for its bioactive natural products for use. Endophytes which are microorganisms that live in plants could be the key in solving this conundrum. Many past studies have shown that endophytes could have the same properties as the host and proven to produce anti-bacterial or anti-cancer compounds. The aims of this study is to characterize the endophytic bacteria isolates which has been isolated from known medicinal plants such as Senna obtusifolia (SK 2283/13), Leea indica (SK 2285/13), Pereskia sacharosa (SK 2286/13) and Isotoma longiflora (SK 2288/13) according their phenotypic traits and genotypic traits. The phenotypic traits that were taken into consideration were the isolation media, the parts of the plants that the isolates were isolated from, the growth conditions such growth rate and temperature, type of gram, antibiogram profile of the isolates, anti-bacterial susceptibility test against bacterial pathogens. The genotypic traits were deduced by using Random Amplification of Polymorphic DNA (RAPD). This study have found that the highest number of isolates were isolated from Senna obtusifolia and the highest number of isolates that were isolated are from the roots of the plants. The best isolation media to be used for isolating the isolates is Starch-Casein Agar. 36 isolates were gram negative and 27 isolates were gram positive in nature. In this study, it was discovered that most of the isolates were susceptible towards Chloramphenicol and resistant towards Gentamicin. The dichloromethane extracts of 28 bacterial isolates shown inhibition towards 9 bacterial pathogens which are Acinobacter baumanii, Bacillus cereus, Bacillus subtilis, Enterobacter cloacae, Klebsiella pneumonia, MRSA, Proteus vulgaris, Salmonella Paratyphi A and Staphylococcus aureus. For the genotypic traits, the genomic DNA of the isolates were amplified successfully by using Primers OPO 10 and OPO 16. In conclusion, the endophytic bacteria isolates were characterized by their phenotypic and genotypic traits and it was found that 9 single isolates clusters which were (ATC)DB3-B, (PDA)IB(B1)-B, (PDA)IF(B1)- B, (SCA)FSe(B1)-B, (PDA)IS(B1)-B, (SCA)IL(B1)-B and (ATC)IF(B1)-B were unique based on both phenotypic and genotypic traits of the isolates. Endophytes Plants, Medicinal 2018-11 Thesis http://psasir.upm.edu.my/id/eprint/103836/ http://psasir.upm.edu.my/id/eprint/103836/1/MOHAMED%20IKHTIFAR%20BIN%20MOHA%20-%20IR.pdf text en public masters Universiti Putra Malaysia Endophytes Plants, Medicinal Cheah, Yoke Kqueen
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Cheah, Yoke Kqueen
topic	Endophytes Endophytes
spellingShingle	Endophytes Endophytes Mohamed Rafi, Mohamed Ikhtifar Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia
description	Malaysia is blessed with rich biodiversity and her people have harnessed this virtue in many ways such as for medicinal uses. However, not all of the plants could be easily grown or harvest for its bioactive natural products for use. Endophytes which are microorganisms that live in plants could be the key in solving this conundrum. Many past studies have shown that endophytes could have the same properties as the host and proven to produce anti-bacterial or anti-cancer compounds. The aims of this study is to characterize the endophytic bacteria isolates which has been isolated from known medicinal plants such as Senna obtusifolia (SK 2283/13), Leea indica (SK 2285/13), Pereskia sacharosa (SK 2286/13) and Isotoma longiflora (SK 2288/13) according their phenotypic traits and genotypic traits. The phenotypic traits that were taken into consideration were the isolation media, the parts of the plants that the isolates were isolated from, the growth conditions such growth rate and temperature, type of gram, antibiogram profile of the isolates, anti-bacterial susceptibility test against bacterial pathogens. The genotypic traits were deduced by using Random Amplification of Polymorphic DNA (RAPD). This study have found that the highest number of isolates were isolated from Senna obtusifolia and the highest number of isolates that were isolated are from the roots of the plants. The best isolation media to be used for isolating the isolates is Starch-Casein Agar. 36 isolates were gram negative and 27 isolates were gram positive in nature. In this study, it was discovered that most of the isolates were susceptible towards Chloramphenicol and resistant towards Gentamicin. The dichloromethane extracts of 28 bacterial isolates shown inhibition towards 9 bacterial pathogens which are Acinobacter baumanii, Bacillus cereus, Bacillus subtilis, Enterobacter cloacae, Klebsiella pneumonia, MRSA, Proteus vulgaris, Salmonella Paratyphi A and Staphylococcus aureus. For the genotypic traits, the genomic DNA of the isolates were amplified successfully by using Primers OPO 10 and OPO 16. In conclusion, the endophytic bacteria isolates were characterized by their phenotypic and genotypic traits and it was found that 9 single isolates clusters which were (ATC)DB3-B, (PDA)IB(B1)-B, (PDA)IF(B1)- B, (SCA)FSe(B1)-B, (PDA)IS(B1)-B, (SCA)IL(B1)-B and (ATC)IF(B1)-B were unique based on both phenotypic and genotypic traits of the isolates.
format	Thesis
qualification_level	Master's degree
author	Mohamed Rafi, Mohamed Ikhtifar
author_facet	Mohamed Rafi, Mohamed Ikhtifar
author_sort	Mohamed Rafi, Mohamed Ikhtifar
title	Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia
title_short	Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia
title_full	Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia
title_fullStr	Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia
title_full_unstemmed	Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia
title_sort	antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of malaysia
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/103836/1/MOHAMED%20IKHTIFAR%20BIN%20MOHA%20-%20IR.pdf
_version_	1776100372216020992

Antibacterial activity and characterization of endophytic bacteria from selected medicinal plants of Malaysia

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