In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption

Human airway system is lined by epithelial cells attached to each other by junctional system. This epithelial barrier can be disrupted by common allergen such as house dust mites (HDM) and consequently resulting in airway diseases such as asthma. Zerumbone was found to possess anti-asthmatic effect...

Full description

Saved in:

Bibliographic Details
Main Author:	Rohhimi, Wafda
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Dust - Prevention & control Mites Asthma
Online Access:	http://psasir.upm.edu.my/id/eprint/103837/1/WAFDA%20BINTI%20ROHHIMI%20-%20IR.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-upm-ir.103837
record_format	uketd_dc
spelling	my-upm-ir.1038372023-05-15T23:53:50Z In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption 2018-10 Rohhimi, Wafda Human airway system is lined by epithelial cells attached to each other by junctional system. This epithelial barrier can be disrupted by common allergen such as house dust mites (HDM) and consequently resulting in airway diseases such as asthma. Zerumbone was found to possess anti-asthmatic effect by modulating Th1/Th2 cytokines. However, there is yet study done to assess whether zerumbone protects the epithelial barrier from junctional disruption before the allergen invades into the immunological barrier of the airway system. This study aims to investigate the effect of zerumbone on HDM-induced airway epithelial barrier disruption. A transformed epithelial cell line derived from human bronchial epithelium (16HBE14o-), which possesses characteristics of in vivo human airway lining, was treated with a range of concentrations of zerumbone (6.25 μM-200 μM) for 24 hours to investigate the non-cytotoxic concentrations of the compound on 16HBE14o- cells. As a result of the cytotoxicity assay, the cells were then co-treated with 100 μg/mL HDM and three selected concentrations of zerumbone (6.25 μM, 12.5 μM and 25 μM) for 24 hours in subsequent experiments. Transepithelial electrical resistance (TEER) assay and FITC-Dextran permeability assay were carried out to study the effect of zerumbone on HDM-induced junctional integrity and permeability of the epithelial monolayer respectively. Zerumbone has shown protective effect on the junctional integrity as all three concentrations (6.25 μM, 12.5 μM and 25 μM) possess significantly lower TEER change and supressed the change by 27%, 48.8% and 49.7% respectively, compared to the HDM group. The permeability assay result complement the result of the TEER assay as 6.25 μM, 12.5 μM and 25 μM of zerumbone shows inhibition in the flux of FITC-Dextran through the epithelial monolayer by 37.8%, 58.6% and 62.2% respectively compared to the HDM group. The localization of junctional proteins, occludin and ZO-1, was studied by using immunofluorescence (IF) while the protein and gene expression were studied by immunoblotting and Real Time-Polymerase Chain Reaction (qPCR) respectively. However, no significant changes can be seen in both protein and gene expression of occludin and ZO-1 as compared to the HDM group. This study has proven that zerumbone preserves both HDM-induced junctional integrity and permeability by maintaining the localization of occludin and ZO-1 without affecting both proteins and gene expression. As a conclusion, zerumbone possesses protective effect on HDM-induced airway epithelial barrier disruption by preserving the junctional permeability and localization without affecting the junctional protein and mRNA expression. This study has proven that zerumbone possess protective effect on the allergen-induced airway barrier disruption therefore enabling it to be further studied as a potential drug for epithelial barrier-related airway inflammatory diseases. Dust - Prevention & control Mites Asthma 2018-10 Thesis http://psasir.upm.edu.my/id/eprint/103837/ http://psasir.upm.edu.my/id/eprint/103837/1/WAFDA%20BINTI%20ROHHIMI%20-%20IR.pdf text en public masters Universiti Putra Malaysia Dust - Prevention & control Mites Asthma Tham, Chau Ling
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English
advisor	Tham, Chau Ling
topic	Dust - Prevention & control Mites Asthma
spellingShingle	Dust - Prevention & control Mites Asthma Rohhimi, Wafda In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption
description	Human airway system is lined by epithelial cells attached to each other by junctional system. This epithelial barrier can be disrupted by common allergen such as house dust mites (HDM) and consequently resulting in airway diseases such as asthma. Zerumbone was found to possess anti-asthmatic effect by modulating Th1/Th2 cytokines. However, there is yet study done to assess whether zerumbone protects the epithelial barrier from junctional disruption before the allergen invades into the immunological barrier of the airway system. This study aims to investigate the effect of zerumbone on HDM-induced airway epithelial barrier disruption. A transformed epithelial cell line derived from human bronchial epithelium (16HBE14o-), which possesses characteristics of in vivo human airway lining, was treated with a range of concentrations of zerumbone (6.25 μM-200 μM) for 24 hours to investigate the non-cytotoxic concentrations of the compound on 16HBE14o- cells. As a result of the cytotoxicity assay, the cells were then co-treated with 100 μg/mL HDM and three selected concentrations of zerumbone (6.25 μM, 12.5 μM and 25 μM) for 24 hours in subsequent experiments. Transepithelial electrical resistance (TEER) assay and FITC-Dextran permeability assay were carried out to study the effect of zerumbone on HDM-induced junctional integrity and permeability of the epithelial monolayer respectively. Zerumbone has shown protective effect on the junctional integrity as all three concentrations (6.25 μM, 12.5 μM and 25 μM) possess significantly lower TEER change and supressed the change by 27%, 48.8% and 49.7% respectively, compared to the HDM group. The permeability assay result complement the result of the TEER assay as 6.25 μM, 12.5 μM and 25 μM of zerumbone shows inhibition in the flux of FITC-Dextran through the epithelial monolayer by 37.8%, 58.6% and 62.2% respectively compared to the HDM group. The localization of junctional proteins, occludin and ZO-1, was studied by using immunofluorescence (IF) while the protein and gene expression were studied by immunoblotting and Real Time-Polymerase Chain Reaction (qPCR) respectively. However, no significant changes can be seen in both protein and gene expression of occludin and ZO-1 as compared to the HDM group. This study has proven that zerumbone preserves both HDM-induced junctional integrity and permeability by maintaining the localization of occludin and ZO-1 without affecting both proteins and gene expression. As a conclusion, zerumbone possesses protective effect on HDM-induced airway epithelial barrier disruption by preserving the junctional permeability and localization without affecting the junctional protein and mRNA expression. This study has proven that zerumbone possess protective effect on the allergen-induced airway barrier disruption therefore enabling it to be further studied as a potential drug for epithelial barrier-related airway inflammatory diseases.
format	Thesis
qualification_level	Master's degree
author	Rohhimi, Wafda
author_facet	Rohhimi, Wafda
author_sort	Rohhimi, Wafda
title	In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption
title_short	In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption
title_full	In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption
title_fullStr	In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption
title_full_unstemmed	In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption
title_sort	in vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption
granting_institution	Universiti Putra Malaysia
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/103837/1/WAFDA%20BINTI%20ROHHIMI%20-%20IR.pdf
_version_	1776100372458242048

In vitro protective effect of zerumbone on house dust mite-induced airway epithelial barrier disruption

Similar Items