In Vitro Propagation Studies on the Initial and Establishment Stages of Rubber (Hevea Brasiliensis Muell. Arg.)
Development of in vitro methods to propagate rubber is highly desirable. Some procedures exist but they generally do not address well the initial and establishment stages. This leads to minimal arrest of two common deterrents in any micropropagation system, i.e., contamination and browning. This...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1997
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10400/1/FP_1997_9_A.pdf |
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| Summary: | Development of in vitro methods to propagate rubber is highly desirable.
Some procedures exist but they generally do not address well the initial and
establishment stages. This leads to minimal arrest of two common deterrents in any
micropropagation system, i.e., contamination and browning. This project attempted
to study both contamination and browning phenomena with an aim of improving
survival without altering regeneration of explants.
Field materials of clone RRIM 600 were used. Experiments on the Initial and
Establishment stages, their interactions and concluding experiments were conducted.
In all experiments, explants were cultured in the standard tissue culture procedures
on basic MS medium.
In the Initial stage, the results showed no significant difference between
chemical treatments of antibiotics and/or fungicide in the field and control. As for the
physical characteristics, efficient initial measures were to use 6-week old middle
portions or shoot tips. This could be improved by growing these explants in a
glasshouse.
In the Establishment stage, the most efficient procedure to decontaminate
explants was Procedure E that included a succession of treatments with ethanol,
Clorox, Rifampicin, Ampicillin, DMSO and Triton-X before culturing. Regarding
debrowning treatments, the best overall responses were obtained by culturing
explants in MS medium (half strength) modified by the addition of 4 mg/L silver
nitrate.
In the analysis of the interactions between treatments in the Initial and
Establishment stages, results showed that the combination of treatments with better
potential was the one that included the use of mature explants (6-week old) surface decontaminated
by Procedure E. |
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