Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production
Biohydrogen production via an anaerobic dark fermentation process at thermophilic conditions is recognized as an excellent biological method and more cost-effective due to its ability to perform without light energy and oxygen source. At thermophilic, this research aims to investigate the effect...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2022
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/104092/1/NUR%20FARAHANA%20BT%20DZUL%20RASHIDI%20-%20IR.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Biohydrogen production via an anaerobic dark fermentation process at thermophilic
conditions is recognized as an excellent biological method and more cost-effective due
to its ability to perform without light energy and oxygen source. At thermophilic, this
research aims to investigate the effect of bacterial immobilization on a matrix made of
alginate and chitosan co-polymerization towards biohydrogen production. In the first
objective, the effect of hydrogen production using granular activated carbon (GAC) as a
microbial support carrier in forming GAC-attached biofilm was evaluated based on
different amounts of sugar concentration as microbial feedstock. The comparison
between initial sugars loading was conducted in a water bath shaker at 120rpm in 800
ml working volume. The acclimatization was operated in a sequencing batch system at
a thermophilic temperature of 60oC and the initial feedstock was set at pH 6. The
fermentation process was continuously carried out until a steady state of biogas was
obtained and it showed the attached-biofilm system successfully stabilizing hydrogen
production after 40 days. The second objective involved the entrapment process in the
formation of GAC-attached biofilm using alginate and chitosan as carrier polymers in
the form of beads. Bacterial immobilization was done by entrapment of GAC-attached
biofilm into 0.5g,1g,2g,3g and 4g of alginate and chitosan respectively (GAC-Alg and
GAC-AlgC). The immobilized beads for both alginate and chitosan were conducted in
batch fermentation using a synthetic medium at a temperature of 60°C, pH 6.0 and in
200 ml working volume. The entrapment of GAC-attached biofilm provides good
support for microorganisms to grow and colonize where high bacterial loads were
observed under a scanning electron microscope (SEM). Lastly, in the final objective, this
research was conducted to assess the performance of GAC-Alg and GAC-AlgC
immobilized beads by using POME as a fermentation medium. It has been observed that
the GAC-Alg immobilized beads resulted in stable hydrogen production after 52 hours
with a consistent HPR of 1.02 mmol H₂/l.h and 1.83 mmol H2/l.h for GAC- AlgC.
Overall, this study showed the immobilization of bacteria-entrapped beads promising
approach to protect the bacteria colonization during the fermentation, thus retaining and promoting microbial growth and protecting the microbial from an unfavourable
environment. |
|---|