Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production
Biohydrogen production via an anaerobic dark fermentation process at thermophilic conditions is recognized as an excellent biological method and more cost-effective due to its ability to perform without light energy and oxygen source. At thermophilic, this research aims to investigate the effect...
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my-upm-ir.1040922023-07-07T03:03:19Z Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production 2022-06 Dzul Rashidi, Nur Farahana Biohydrogen production via an anaerobic dark fermentation process at thermophilic conditions is recognized as an excellent biological method and more cost-effective due to its ability to perform without light energy and oxygen source. At thermophilic, this research aims to investigate the effect of bacterial immobilization on a matrix made of alginate and chitosan co-polymerization towards biohydrogen production. In the first objective, the effect of hydrogen production using granular activated carbon (GAC) as a microbial support carrier in forming GAC-attached biofilm was evaluated based on different amounts of sugar concentration as microbial feedstock. The comparison between initial sugars loading was conducted in a water bath shaker at 120rpm in 800 ml working volume. The acclimatization was operated in a sequencing batch system at a thermophilic temperature of 60oC and the initial feedstock was set at pH 6. The fermentation process was continuously carried out until a steady state of biogas was obtained and it showed the attached-biofilm system successfully stabilizing hydrogen production after 40 days. The second objective involved the entrapment process in the formation of GAC-attached biofilm using alginate and chitosan as carrier polymers in the form of beads. Bacterial immobilization was done by entrapment of GAC-attached biofilm into 0.5g,1g,2g,3g and 4g of alginate and chitosan respectively (GAC-Alg and GAC-AlgC). The immobilized beads for both alginate and chitosan were conducted in batch fermentation using a synthetic medium at a temperature of 60°C, pH 6.0 and in 200 ml working volume. The entrapment of GAC-attached biofilm provides good support for microorganisms to grow and colonize where high bacterial loads were observed under a scanning electron microscope (SEM). Lastly, in the final objective, this research was conducted to assess the performance of GAC-Alg and GAC-AlgC immobilized beads by using POME as a fermentation medium. It has been observed that the GAC-Alg immobilized beads resulted in stable hydrogen production after 52 hours with a consistent HPR of 1.02 mmol H₂/l.h and 1.83 mmol H2/l.h for GAC- AlgC. Overall, this study showed the immobilization of bacteria-entrapped beads promising approach to protect the bacteria colonization during the fermentation, thus retaining and promoting microbial growth and protecting the microbial from an unfavourable environment. Biofilms Carbon, Activated Microbial polysaccharides 2022-06 Thesis http://psasir.upm.edu.my/id/eprint/104092/ http://psasir.upm.edu.my/id/eprint/104092/1/NUR%20FARAHANA%20BT%20DZUL%20RASHIDI%20-%20IR.pdf text en public masters Universiti Putra Malaysia Biofilms Carbon, Activated Microbial polysaccharides Jamali, Nur Syakina |
| institution |
Universiti Putra Malaysia |
| collection |
PSAS Institutional Repository |
| language |
English |
| advisor |
Jamali, Nur Syakina |
| topic |
Biofilms Biofilms Microbial polysaccharides |
| spellingShingle |
Biofilms Biofilms Microbial polysaccharides Dzul Rashidi, Nur Farahana Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| description |
Biohydrogen production via an anaerobic dark fermentation process at thermophilic
conditions is recognized as an excellent biological method and more cost-effective due
to its ability to perform without light energy and oxygen source. At thermophilic, this
research aims to investigate the effect of bacterial immobilization on a matrix made of
alginate and chitosan co-polymerization towards biohydrogen production. In the first
objective, the effect of hydrogen production using granular activated carbon (GAC) as a
microbial support carrier in forming GAC-attached biofilm was evaluated based on
different amounts of sugar concentration as microbial feedstock. The comparison
between initial sugars loading was conducted in a water bath shaker at 120rpm in 800
ml working volume. The acclimatization was operated in a sequencing batch system at
a thermophilic temperature of 60oC and the initial feedstock was set at pH 6. The
fermentation process was continuously carried out until a steady state of biogas was
obtained and it showed the attached-biofilm system successfully stabilizing hydrogen
production after 40 days. The second objective involved the entrapment process in the
formation of GAC-attached biofilm using alginate and chitosan as carrier polymers in
the form of beads. Bacterial immobilization was done by entrapment of GAC-attached
biofilm into 0.5g,1g,2g,3g and 4g of alginate and chitosan respectively (GAC-Alg and
GAC-AlgC). The immobilized beads for both alginate and chitosan were conducted in
batch fermentation using a synthetic medium at a temperature of 60°C, pH 6.0 and in
200 ml working volume. The entrapment of GAC-attached biofilm provides good
support for microorganisms to grow and colonize where high bacterial loads were
observed under a scanning electron microscope (SEM). Lastly, in the final objective, this
research was conducted to assess the performance of GAC-Alg and GAC-AlgC
immobilized beads by using POME as a fermentation medium. It has been observed that
the GAC-Alg immobilized beads resulted in stable hydrogen production after 52 hours
with a consistent HPR of 1.02 mmol H₂/l.h and 1.83 mmol H2/l.h for GAC- AlgC.
Overall, this study showed the immobilization of bacteria-entrapped beads promising
approach to protect the bacteria colonization during the fermentation, thus retaining and promoting microbial growth and protecting the microbial from an unfavourable
environment. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Dzul Rashidi, Nur Farahana |
| author_facet |
Dzul Rashidi, Nur Farahana |
| author_sort |
Dzul Rashidi, Nur Farahana |
| title |
Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_short |
Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_full |
Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_fullStr |
Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_full_unstemmed |
Evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| title_sort |
evaluation of granular-activated carbon-attached biofilm coated in alginate-chitosan for biohydrogen production |
| granting_institution |
Universiti Putra Malaysia |
| publishDate |
2022 |
| url |
http://psasir.upm.edu.my/id/eprint/104092/1/NUR%20FARAHANA%20BT%20DZUL%20RASHIDI%20-%20IR.pdf |
| _version_ |
1776100409160499200 |