Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion

Enhancement of the biocontrol activities of Trichoderma harzianum Rifai against two soilborne pathogens, Sclerotium rolfsii and Ganoderma boninense through protoplast fusion was attempted. Mycelial cultures from three indigenous isolates from the rhizospheres of groundnut (IMI 378843), chilli (I...

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Main Author:	Wong, Mui Yun
Format:	Thesis
Language:	English English
Published:	1999
Subjects:	Trichoderma
Online Access:	http://psasir.upm.edu.my/id/eprint/10423/1/FP_1999_2_A.pdf
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id	my-upm-ir.10423
record_format	uketd_dc
spelling	my-upm-ir.104232011-04-11T02:40:25Z Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion 1999 Wong, Mui Yun Enhancement of the biocontrol activities of Trichoderma harzianum Rifai against two soilborne pathogens, Sclerotium rolfsii and Ganoderma boninense through protoplast fusion was attempted. Mycelial cultures from three indigenous isolates from the rhizospheres of groundnut (IMI 378843), chilli (IMI 378844) and oil palm (IMI 378841) were used for the protoplast isolation and fusion studies. The result showed that the optimum release of viable protoplasts was obtained when mycelial cultures at the exponential stage was incubated for 4 h in Novozym 234 (Sigma) as the lytic enzyme at concentration of 7 mg/ml dissolved in 0.7 M NaCI and 0.6 M sorbitol. Pretreatment of mycelium v.ith 0.01 M 2-mercaptoethanol gave no Significant difference on protoplast yield of the three isolates studied. The protoplast yield was within the range of 10⁶-10⁸ protoplasts/ml and the average size of the protoplasts was 2.5-10.0 µm. Chemically induced fusion, using polyethylene glycol (pEG), among the three isolates yielded a total of 12 fusants. germinated 18 h after incubation in liquid Protoplast Regeneration Medium (PRM). When plated on solid PRM, the fusants regenerated into single colonies between 24-48 h after incubation. Of the 12 fusants obtained, five fusants showed non-parental type in isozyme analysis. They were further evaluated based on the cultural and morphological analysis, biomass growth, antagonistic activities against S. Tolfsii and G. boninense, tolerance to commonly used fungicides, and the ability to produce two extracellular lytic enzymes, B-l,3-glucanase and chitinase. Trichoderma 1999 Thesis http://psasir.upm.edu.my/id/eprint/10423/ http://psasir.upm.edu.my/id/eprint/10423/1/FP_1999_2_A.pdf application/pdf en public masters Universiti Putra Malaysia Trichoderma Faculty of Agriculture English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Trichoderma
spellingShingle	Trichoderma Wong, Mui Yun Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion
description	Enhancement of the biocontrol activities of Trichoderma harzianum Rifai against two soilborne pathogens, Sclerotium rolfsii and Ganoderma boninense through protoplast fusion was attempted. Mycelial cultures from three indigenous isolates from the rhizospheres of groundnut (IMI 378843), chilli (IMI 378844) and oil palm (IMI 378841) were used for the protoplast isolation and fusion studies. The result showed that the optimum release of viable protoplasts was obtained when mycelial cultures at the exponential stage was incubated for 4 h in Novozym 234 (Sigma) as the lytic enzyme at concentration of 7 mg/ml dissolved in 0.7 M NaCI and 0.6 M sorbitol. Pretreatment of mycelium v.ith 0.01 M 2-mercaptoethanol gave no Significant difference on protoplast yield of the three isolates studied. The protoplast yield was within the range of 10⁶-10⁸ protoplasts/ml and the average size of the protoplasts was 2.5-10.0 µm. Chemically induced fusion, using polyethylene glycol (pEG), among the three isolates yielded a total of 12 fusants. germinated 18 h after incubation in liquid Protoplast Regeneration Medium (PRM). When plated on solid PRM, the fusants regenerated into single colonies between 24-48 h after incubation. Of the 12 fusants obtained, five fusants showed non-parental type in isozyme analysis. They were further evaluated based on the cultural and morphological analysis, biomass growth, antagonistic activities against S. Tolfsii and G. boninense, tolerance to commonly used fungicides, and the ability to produce two extracellular lytic enzymes, B-l,3-glucanase and chitinase.
format	Thesis
qualification_level	Master's degree
author	Wong, Mui Yun
author_facet	Wong, Mui Yun
author_sort	Wong, Mui Yun
title	Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion
title_short	Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion
title_full	Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion
title_fullStr	Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion
title_full_unstemmed	Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion
title_sort	enhancement of biocontrol activities of trichoderma harzianum rifai through protoplast fusion
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/10423/1/FP_1999_2_A.pdf
_version_	1747811118437367808

Enhancement of Biocontrol Activities of Trichoderma Harzianum Rifai Through Protoplast Fusion

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