In Vitro Production and Infectivity of Oncobasidium Theobromae Talbot and Keane Basidiospores
In vitro sporulation of Oncobasidium theobromae Talbot & Keane was induced using a modified version of the system by Shari Fudin (1995) by exposing 14 day old cultures to saturated moist air from a humidifier. Basidiospore production was able to be repeated consistently using the new system w...
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| Format: | Thesis |
| Language: | English English |
| Published: |
1999
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10424/1/FP_1999_3.pdf |
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| Summary: | In vitro sporulation of Oncobasidium theobromae Talbot & Keane was
induced using a modified version of the system by Shari Fudin (1995) by exposing
14 day old cultures to saturated moist air from a humidifier. Basidiospore
production was able to be repeated consistently using the new system where a black
perspex cabinet was used as an enclosure but the number of spores produced was
low (not exceeding 36,300 spores per ml). Sporulation occurred when cultures
were exposed to a mean relative humidity above 90% and low mean temperature
(< 26°C). Duration of exposure to saturated moist air was vital for in vitro
sporulation. O. theobromae cultures produced spores when exposed to continuous
saturated moist air for more than 240 mins/day but significantly higher counts and
faster formation of spores were achieved at exposures of 480 mins/day to saturated
moist air. 14 days old cultures gave high spore counts.
Observations indicated that the presence of monilioid hyphae was necessary
for sporulation of O. theobromae since the basidium arises from it, but the
formation did not appear to be influenced by factors of sporulation like saturated moist air. There was no significant difference in the formation of monilioid hyphae
when the duration of exposure of cultures to saturated moist air was varied. Basidia
only formed when the cultures were exposed to more than 240 mins/day of saturated
moist air but the duration of exposure to saturated moist air did not appear to
influence the quantity of basidia formed in cultures. Sporulation was improved by
maintaining the modified system under controlled environmental conditions with
the temperatures between 21°C-23°C and the mean relative humidity above 90%.
Cultures were exposed to continuous saturated moist air for 480 mins/day. The
spore production under these conditions was consistent with mean spore counts
ranging from 62,300 spores/ml to 120,000 spores/ml. The spores produced were
viable as confirmed by an average spore germination of 46% and 57.5% after 12
hours and 24 hours incubation in the laboratory |
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