Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids

Mutation breeding is an alternative strategy to conventional breeding. The physical mutagen, gamma (y) radiation, has long been used in various mutation breeding programmes. In this connection it is necessary to determine the optimum dose before starting any mutation breeding programme. In vitro...

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Main Author:	Ling, Adrain Chieng Kuang
Format:	Thesis
Language:	English English
Published:	1999
Subjects:	Gamma rays Radiation
Online Access:	http://psasir.upm.edu.my/id/eprint/10437/1/FP_1999_15_A.pdf
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id	my-upm-ir.10437
record_format	uketd_dc
spelling	my-upm-ir.104372011-07-25T01:48:26Z Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids 1999 Ling, Adrain Chieng Kuang Mutation breeding is an alternative strategy to conventional breeding. The physical mutagen, gamma (y) radiation, has long been used in various mutation breeding programmes. In this connection it is necessary to determine the optimum dose before starting any mutation breeding programme. In vitro cultured protocorm-like bodies (PLBs) of 2 orchid hybrids, Mokara Chark Kuan and Dendrobium Jacky were used to study the effects of yirradiation and to determine the optimum dose. The PLBs were irradiated with y-rays at different doses of 0, 25, 50, 75, 100, 125 and 150 Grays (Gy) in Set I and 0, 20, 40, 60 and 80 Gy in Set II of the experiment. A senes of observations on the effects of y-irradiation were recorded. Effects on genomic DNA were also observed. The present study showed that irradiation resulted in various degrees of lethality to in vitro cultures of both Mokara Chark Kuan and Dendrobium Jacky. Decrease in survival with increasing doses of y-irradiation was significantly observed. Optimum dose of y-irradiation for in vitro cultures of Mokara Chark Kuan and Dendrobium Jacky were estimated to be in the range of 20-40 Gy and 60-70 Gy respectively. Regeneration of plantlets was found to decline when higher irradiation doses were applied. Irradiation also has inhibited regeneration of in vitro cultures of both hybrids. Gamma rays Plants, Effect of Gamma rays Radiation 1999 Thesis http://psasir.upm.edu.my/id/eprint/10437/ http://psasir.upm.edu.my/id/eprint/10437/1/FP_1999_15_A.pdf application/pdf en public masters Universiti Putra Malaysia Gamma rays Plants, Effect of Gamma rays Radiation Faculty of Agriculture English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
topic	Gamma rays Gamma rays Radiation
spellingShingle	Gamma rays Gamma rays Radiation Ling, Adrain Chieng Kuang Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids
description	Mutation breeding is an alternative strategy to conventional breeding. The physical mutagen, gamma (y) radiation, has long been used in various mutation breeding programmes. In this connection it is necessary to determine the optimum dose before starting any mutation breeding programme. In vitro cultured protocorm-like bodies (PLBs) of 2 orchid hybrids, Mokara Chark Kuan and Dendrobium Jacky were used to study the effects of yirradiation and to determine the optimum dose. The PLBs were irradiated with y-rays at different doses of 0, 25, 50, 75, 100, 125 and 150 Grays (Gy) in Set I and 0, 20, 40, 60 and 80 Gy in Set II of the experiment. A senes of observations on the effects of y-irradiation were recorded. Effects on genomic DNA were also observed. The present study showed that irradiation resulted in various degrees of lethality to in vitro cultures of both Mokara Chark Kuan and Dendrobium Jacky. Decrease in survival with increasing doses of y-irradiation was significantly observed. Optimum dose of y-irradiation for in vitro cultures of Mokara Chark Kuan and Dendrobium Jacky were estimated to be in the range of 20-40 Gy and 60-70 Gy respectively. Regeneration of plantlets was found to decline when higher irradiation doses were applied. Irradiation also has inhibited regeneration of in vitro cultures of both hybrids.
format	Thesis
qualification_level	Master's degree
author	Ling, Adrain Chieng Kuang
author_facet	Ling, Adrain Chieng Kuang
author_sort	Ling, Adrain Chieng Kuang
title	Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids
title_short	Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids
title_full	Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids
title_fullStr	Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids
title_full_unstemmed	Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids
title_sort	effects of gamma irradiation on in vitro cultures of selected orchid hybrids
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	1999
url	http://psasir.upm.edu.my/id/eprint/10437/1/FP_1999_15_A.pdf
_version_	1747811121637621760

Effects of Gamma Irradiation on in Vitro Cultures of Selected Orchid Hybrids

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