Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification

The present study was conducted to develop a protocol for long-term preservation of orchid germplasm. Protocorm-like bodies (PLBs) of orchid hybrid Dendrobium Kasem Boonchoo White were subjected to different treatments of preculture, cryoprotection and dehydration procedures of vitrification tech...

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Main Author:	Sipen, Philip
Format:	Thesis
Language:	English English
Published:	2000
Subjects:	Orchids - Breeding Plant hybridization
Online Access:	http://psasir.upm.edu.my/id/eprint/10521/1/FP_2000_14.pdf
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id	my-upm-ir.10521
record_format	uketd_dc
spelling	my-upm-ir.105212024-04-03T07:24:53Z Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification 2000-08 Sipen, Philip The present study was conducted to develop a protocol for long-term preservation of orchid germplasm. Protocorm-like bodies (PLBs) of orchid hybrid Dendrobium Kasem Boonchoo White were subjected to different treatments of preculture, cryoprotection and dehydration procedures of vitrification technique. The PLBs were precultured on Vacin and Went medium supplemented with 0, 0.06,0.1,0.3,0.5 and 0.7 M sucrose for 3 days. Preculturing with 0.06 to 0.5 M sucrose gave 3.7 to 23.3% viability after cryopreservation. No viable PLBs were found from 0 and 0.7 M sucrose treatments. None of the PLBs survived cryopreservation in all the sucrose concentrations tested. The PLBs were precultured with 0.3 M sucrose for 0, 1, 3, 5, 7 and 9 days. Viability ranged from 11.1 to 26.3% after cryopreservation from 1 to 9 days treatments. No viability was recorded after cryopreservation from treatment without sucrose preculture. There was no survival recorded in all treatments after cryopreservation. When precultured PLBs were cryoprotected with different loading solutions (LSI, LS2, LS3, LS4, LS5 and LS6) for 15 min, all treatments gave 7.4 to 34.4% viability after cryopreservation. All treatments gave 3.7 to 11.1% survival after cryopreservation except LS 1 and LS2 treatments. PLBs were cryoprotected with LS5 for 0, 5, 10, 15, 20, 25 and 30 min. Viability of 7.4 to 36.8% were recorded after cryopreservation when cryoprotected for 5 to 30 min. Without cryoprotection, viability was zero after cryopreservation. The survival rates of 11.1 to 30.3% were obtained after cryopreservation from 5 to 25 min treatments. Cryoprotection for 30 min and without cryoprotection did not give any survival at all after cryopreservation. Cryoprotected PLBs were dehydrated with different vitrification solutions (VS 1, VS2, VS3, VS4 and VS5) for 10 min. All vitrification solutions gave 11.1 to 39.2% viability after cryopreservation. Survival of 7.4 to 26.3% after cryopreservation were recorded in all treatments except VS 1. Orchids - Breeding Plant hybridization 2000-08 Thesis http://psasir.upm.edu.my/id/eprint/10521/ http://psasir.upm.edu.my/id/eprint/10521/1/FP_2000_14.pdf text en public masters Universiti Putra Malaysia Orchids - Breeding Plant hybridization Faculty of Agriculture Kadzimin, Saleh English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Kadzimin, Saleh
topic	Orchids - Breeding Plant hybridization
spellingShingle	Orchids - Breeding Plant hybridization Sipen, Philip Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification
description	The present study was conducted to develop a protocol for long-term preservation of orchid germplasm. Protocorm-like bodies (PLBs) of orchid hybrid Dendrobium Kasem Boonchoo White were subjected to different treatments of preculture, cryoprotection and dehydration procedures of vitrification technique. The PLBs were precultured on Vacin and Went medium supplemented with 0, 0.06,0.1,0.3,0.5 and 0.7 M sucrose for 3 days. Preculturing with 0.06 to 0.5 M sucrose gave 3.7 to 23.3% viability after cryopreservation. No viable PLBs were found from 0 and 0.7 M sucrose treatments. None of the PLBs survived cryopreservation in all the sucrose concentrations tested. The PLBs were precultured with 0.3 M sucrose for 0, 1, 3, 5, 7 and 9 days. Viability ranged from 11.1 to 26.3% after cryopreservation from 1 to 9 days treatments. No viability was recorded after cryopreservation from treatment without sucrose preculture. There was no survival recorded in all treatments after cryopreservation. When precultured PLBs were cryoprotected with different loading solutions (LSI, LS2, LS3, LS4, LS5 and LS6) for 15 min, all treatments gave 7.4 to 34.4% viability after cryopreservation. All treatments gave 3.7 to 11.1% survival after cryopreservation except LS 1 and LS2 treatments. PLBs were cryoprotected with LS5 for 0, 5, 10, 15, 20, 25 and 30 min. Viability of 7.4 to 36.8% were recorded after cryopreservation when cryoprotected for 5 to 30 min. Without cryoprotection, viability was zero after cryopreservation. The survival rates of 11.1 to 30.3% were obtained after cryopreservation from 5 to 25 min treatments. Cryoprotection for 30 min and without cryoprotection did not give any survival at all after cryopreservation. Cryoprotected PLBs were dehydrated with different vitrification solutions (VS 1, VS2, VS3, VS4 and VS5) for 10 min. All vitrification solutions gave 11.1 to 39.2% viability after cryopreservation. Survival of 7.4 to 26.3% after cryopreservation were recorded in all treatments except VS 1.
format	Thesis
qualification_level	Master's degree
author	Sipen, Philip
author_facet	Sipen, Philip
author_sort	Sipen, Philip
title	Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification
title_short	Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification
title_full	Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification
title_fullStr	Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification
title_full_unstemmed	Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification
title_sort	cryopreservation of protocorm-like bodies of orchid hybrid dendrobium kasem boonchoo white by vitrification
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2000
url	http://psasir.upm.edu.my/id/eprint/10521/1/FP_2000_14.pdf
_version_	1804888559251881984

Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification

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