Induction and Plant Regeneration of Callus in Dendrobium
The present study examines the effects of some selected chemicals and physical treatments on protocorm-like body (plbs) of orchid hybrid, Dendrobium Kasem White . This is an attempt at initiating embryogenic callus, and setting conditions optimal for its maintenance and regeneration into plbs an...
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| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
2000
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/10522/1/FP_2000_15_A.pdf |
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| Summary: | The present study examines the effects of some selected chemicals
and physical treatments on protocorm-like body (plbs) of orchid
hybrid, Dendrobium Kasem White . This is an attempt at initiating
embryogenic callus, and setting conditions optimal for its
maintenance and regeneration into plbs and hence plantlets. This
protocol was initiated to establish a system for the transfer of genetic
material through genetic engineering technology.
The study was conducted in four main parts, a set of preliminary
studies, callus induction, maintenance and regeneration. The
critical concentration of picloram and kinetin for the survival of
wounded and unwounded plbs were obtained from the preliminary
studies. Optimal concentration of picloram and kinetin for
unwounded plbs ranged from 0 to 1.0 mg/l. For wounded plbs the concentration ranged from 0 to 0.5 mg/l and 0 to 1 mg/l for
picloram and kinetin respectively.
Callus-like tissue formation was observed from unwounded plbs
cultured on % MS supplemented with 0.6 to 0.9mg/l picloram and
0.3 to 0.6 mg/l kinetin treatments. The best concentrations were
0.75 mg/l picloram and 0 .60 mg/l kinetin. Induction was achieved
in the 40-day dark treatment. Wounded plbs produced callus-like
tissue on 0.1 mg/l picloram + 0.8 mg/l kinetin and 0 mg/l picloram
+ 1.0 mg/l kinetin treatments after 30 days of culture in the dark.
Callus-like tissue remained viable in both solid and liquid medium of
% MS supplemented with 0.75 mg/l picloram and 0.6 mg/l kinetin,
cultured in the dark.
Through several modifications of medium, the callus-like tissue
regenerated into plbs when the medium was devoid of hormones and
cultured in the light. Regenerated plbs formed shoots and roots
upon transfer to medium with lBA and BA. |
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