Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense

A study on the distribution of Basal Stem Rot (BSR), frequency of isolation of Trichoderma spp. from oil palm rhizosphere, its biological activities and variations was attempted The incidence of BSR was found to be correlated to age of palm. The percentage of disease incidence (PDI) in mature pal...

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Main Author:	Joseph, Hendry
Format:	Thesis
Language:	English English
Published:	2000
Subjects:	Trichoderma Oil palm - Research Ganoderma
Online Access:	http://psasir.upm.edu.my/id/eprint/10524/1/FP_2000_16.pdf
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id	my-upm-ir.10524
record_format	uketd_dc
spelling	my-upm-ir.105242024-04-03T07:46:08Z Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense 2000-05 Joseph, Hendry A study on the distribution of Basal Stem Rot (BSR), frequency of isolation of Trichoderma spp. from oil palm rhizosphere, its biological activities and variations was attempted The incidence of BSR was found to be correlated to age of palm. The percentage of disease incidence (PDI) in mature palm (OP74) was comparatively higher than the middle age palm (OP89) or young palm (OP94). Frequency of isolation of Trichoderma spp. in the oil palm rhizosphere was found to be correlated to age of palm and disease incidence. Frequency of isolation (cfu/g soil) of Trichoderma spp. was higher in OP74 than OP89 or OP94. Four main species aggregates identified from oil palm rhizosphere were Trichoderma harzianum, Trichoderma virens, Trichoderma koningii and Trichoderma longibrachiatum. T. harzianum was the highest in distribution in all the areas sampled with T. longibrachiatum being the lowest in its population dynamic. In-vitro studies showed there were no variations in antagonistic activities between Trichoderma species aggregates. Meanwhile, a significant difference was observed within species aggregate as tested by dual culture and colony degradation tests, and production of volatile and non-volatile substances. Isolates TH1 of T. harzianum and TV1 of T. virens were observed to be consistent in their antagonistic and parasitic activities. Variations between and within species aggregate were studied usmg intracellular isozyme and RAPD-PCR. Peroxidase activity was able to identify variations between species aggregates, however less sensitive to detect variations within intraspecific groups. RAPD was useful and able to detect variations within intraspecific groups, however was not able to identify variations between species aggregates T. virens could hold the possibility to be develop as a potential biopesticide based on its diverse genetic nature and biological activities. Trichoderma Oil palm - Research Ganoderma 2000-05 Thesis http://psasir.upm.edu.my/id/eprint/10524/ http://psasir.upm.edu.my/id/eprint/10524/1/FP_2000_16.pdf text en public masters Universiti Putra Malaysia Trichoderma Oil palm - Research Ganoderma Faculty of Agriculture Meon, Sariah English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Meon, Sariah
topic	Trichoderma Oil palm - Research Ganoderma
spellingShingle	Trichoderma Oil palm - Research Ganoderma Joseph, Hendry Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense
description	A study on the distribution of Basal Stem Rot (BSR), frequency of isolation of Trichoderma spp. from oil palm rhizosphere, its biological activities and variations was attempted The incidence of BSR was found to be correlated to age of palm. The percentage of disease incidence (PDI) in mature palm (OP74) was comparatively higher than the middle age palm (OP89) or young palm (OP94). Frequency of isolation of Trichoderma spp. in the oil palm rhizosphere was found to be correlated to age of palm and disease incidence. Frequency of isolation (cfu/g soil) of Trichoderma spp. was higher in OP74 than OP89 or OP94. Four main species aggregates identified from oil palm rhizosphere were Trichoderma harzianum, Trichoderma virens, Trichoderma koningii and Trichoderma longibrachiatum. T. harzianum was the highest in distribution in all the areas sampled with T. longibrachiatum being the lowest in its population dynamic. In-vitro studies showed there were no variations in antagonistic activities between Trichoderma species aggregates. Meanwhile, a significant difference was observed within species aggregate as tested by dual culture and colony degradation tests, and production of volatile and non-volatile substances. Isolates TH1 of T. harzianum and TV1 of T. virens were observed to be consistent in their antagonistic and parasitic activities. Variations between and within species aggregate were studied usmg intracellular isozyme and RAPD-PCR. Peroxidase activity was able to identify variations between species aggregates, however less sensitive to detect variations within intraspecific groups. RAPD was useful and able to detect variations within intraspecific groups, however was not able to identify variations between species aggregates T. virens could hold the possibility to be develop as a potential biopesticide based on its diverse genetic nature and biological activities.
format	Thesis
qualification_level	Master's degree
author	Joseph, Hendry
author_facet	Joseph, Hendry
author_sort	Joseph, Hendry
title	Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense
title_short	Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense
title_full	Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense
title_fullStr	Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense
title_full_unstemmed	Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense
title_sort	variations in trichoderma from oil palm rhizosphere and its biological activities against ganoderma boninense
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2000
url	http://psasir.upm.edu.my/id/eprint/10524/1/FP_2000_16.pdf
_version_	1804888560008953856

Variations in Trichoderma from Oil Palm Rhizosphere and its Biological Activities Against Ganoderma Boninense

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