Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer

Biological nitrogen fixation (BNF) studies have been emphasized following the problem faced in the application of chemical nitrogen fertilizer in wetland rice cultivation. Among fertilizer inputs, nitrogen is the major limiting nutrient for crop production. Besides energy intensive, chemical nitr...

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Main Author:	Tan, Geok Hun
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Rhizobacteria - Isolation & purification Biofertilizers Rice - Fertilizers
Online Access:	http://psasir.upm.edu.my/id/eprint/10542/1/FP_2001_2.pdf
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id	my-upm-ir.10542
record_format	uketd_dc
spelling	my-upm-ir.105422024-04-15T07:43:29Z Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer 2001-12 Tan, Geok Hun Biological nitrogen fixation (BNF) studies have been emphasized following the problem faced in the application of chemical nitrogen fertilizer in wetland rice cultivation. Among fertilizer inputs, nitrogen is the major limiting nutrient for crop production. Besides energy intensive, chemical nitrogen fertilizer also cause groundwater pollution. One of the approaches to alleviate this problem is by isolating the beneficial rhizobacterial from wetland rice which have the capability to fix nitrogen biologically and produce plant growth hormones, indole-3-acetic acid (1M) in vitro. The study consisted of three experiments. Experiment I was the isolation and identification of rhizobacterial strains from paddy land and their reactions toward several biochemical tests. Experiment II was the determination of 1M production by the rhizobacterial using modified colorimetric methods. Experiment III was to determine the effects of 1M production by rhizobacterial isolates on growth of rice variety MR 211, the colonization of these isolates on rice roots and nitrogenase activity of rhizobacteria through Acetylene Reduction Assay. From the isolation procedures, several strains of bacterial were isolated from the rice rhizosphere. The population of these rhizobacterial ranged from 105 to 109 cfu/mL. By using Biolog Identification System, these rhizobacterial have been identified as Corynebacterium spp., Proteus mirabilis and Spirillum spp., which showed their unique characteristics to Gram staining, pellicle formation, motility, starch hydrolysis, catalase, antibiotic test and carbohydrate fermentation with different carbon sources. The experimental results indicate that Corynebacterium spp., Proteus mirabilis and Spirillum spp. were able to produce 1M in culture medium supplemented with L-tryptophan as a precursor, which ranged between 1-10 J-lg/mL. The presence of precursor was essential for detection of 1M production by the bacteria. 1M production increased in the presence of 0.1 mg/L, 10 mg/L and 100 mg/L of precursor. Rhizobacteria - Isolation & purification Biofertilizers Rice - Fertilizers 2001-12 Thesis http://psasir.upm.edu.my/id/eprint/10542/ http://psasir.upm.edu.my/id/eprint/10542/1/FP_2001_2.pdf text en public masters Universiti Putra Malaysia Rhizobacteria - Isolation & purification Biofertilizers Rice - Fertilizers Faculty of Agriculture Mohd Saud, Halimi English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Mohd Saud, Halimi
topic	Rhizobacteria - Isolation & purification Biofertilizers Rice - Fertilizers
spellingShingle	Rhizobacteria - Isolation & purification Biofertilizers Rice - Fertilizers Tan, Geok Hun Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer
description	Biological nitrogen fixation (BNF) studies have been emphasized following the problem faced in the application of chemical nitrogen fertilizer in wetland rice cultivation. Among fertilizer inputs, nitrogen is the major limiting nutrient for crop production. Besides energy intensive, chemical nitrogen fertilizer also cause groundwater pollution. One of the approaches to alleviate this problem is by isolating the beneficial rhizobacterial from wetland rice which have the capability to fix nitrogen biologically and produce plant growth hormones, indole-3-acetic acid (1M) in vitro. The study consisted of three experiments. Experiment I was the isolation and identification of rhizobacterial strains from paddy land and their reactions toward several biochemical tests. Experiment II was the determination of 1M production by the rhizobacterial using modified colorimetric methods. Experiment III was to determine the effects of 1M production by rhizobacterial isolates on growth of rice variety MR 211, the colonization of these isolates on rice roots and nitrogenase activity of rhizobacteria through Acetylene Reduction Assay. From the isolation procedures, several strains of bacterial were isolated from the rice rhizosphere. The population of these rhizobacterial ranged from 105 to 109 cfu/mL. By using Biolog Identification System, these rhizobacterial have been identified as Corynebacterium spp., Proteus mirabilis and Spirillum spp., which showed their unique characteristics to Gram staining, pellicle formation, motility, starch hydrolysis, catalase, antibiotic test and carbohydrate fermentation with different carbon sources. The experimental results indicate that Corynebacterium spp., Proteus mirabilis and Spirillum spp. were able to produce 1M in culture medium supplemented with L-tryptophan as a precursor, which ranged between 1-10 J-lg/mL. The presence of precursor was essential for detection of 1M production by the bacteria. 1M production increased in the presence of 0.1 mg/L, 10 mg/L and 100 mg/L of precursor.
format	Thesis
qualification_level	Master's degree
author	Tan, Geok Hun
author_facet	Tan, Geok Hun
author_sort	Tan, Geok Hun
title	Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer
title_short	Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer
title_full	Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer
title_fullStr	Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer
title_full_unstemmed	Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer
title_sort	isolation and identification of rhizobacteria from paddy land and their benefit as a biofertilizer
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/10542/1/FP_2001_2.pdf
_version_	1804888564314406912

Isolation and Identification of Rhizobacteria from Paddy Land and Their Benefit as a Biofertilizer

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