Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)

This study was carried out with the main objective of establishing somatic embryo production in papaya that may be used for further genetic improvement of the crop. The specific objectives were to induce somatic embryogenesis from immature zygotic embryos, to establish cell suspension culture of...

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Main Author:	Munari, Agus Sutanto
Format:	Thesis
Language:	English English
Published:	2001
Subjects:	Papaya - Somatic embryogenesis - Case studies
Online Access:	http://psasir.upm.edu.my/id/eprint/10559/1/FP_2001_16.pdf
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id	my-upm-ir.10559
record_format	uketd_dc
spelling	my-upm-ir.105592024-04-17T02:21:07Z Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.) 2001-09 Munari, Agus Sutanto This study was carried out with the main objective of establishing somatic embryo production in papaya that may be used for further genetic improvement of the crop. The specific objectives were to induce somatic embryogenesis from immature zygotic embryos, to establish cell suspension culture of somatic embryos and to isolate and culture protoplasts from in vitro leaves. The first experiment studied the effect of growth regulators on the induction and regeneration of somatic embryos from immature zygotic embryos. The combinations of 2,4-dichlorophenoxyacetic acid (2,4-0) and 6-benzylaminopurine (BAP) at various concentrations were assessed in order to determine the best combination for somatic embryogenesis. The experiment was conducted in a Completely Randomized Design. The results showed that higher 2,4-D with or without BAP in the medium increased the percentage of somatic embryo formation and the number of somatic embryos per explant. MS medium supplemented with 5.0 mg/L 2,4-D promoted the highest percentage of somatic embryogenesis (74.55%), while the combinati on of 1.0 mg/L 2,4-0 and 0.01 mg/L BAP produced the highest percentage of callus formation (52. 58%). The highest number of somatic embryos per explant (66.61) was obtained when 5.0 mg/L 2,4-D and 0.01 mg/L BAP were added into MS medium. For germinati on of somatic embryos, the result was very inconsistent; nevertheless, the best treatment for plantlet formation was MS medium without growth hormone (NoBo). The second experiment was the establishment of cell suspensi on culture of somatic embryos. Four weeks after the transfer of somatic embryos into liquid MS medium containing 2.0 mg/L 2,4-0, pro-embryogenic masses (PEMs) were formed in the suspension. Maturation of embryos was achieved on transferring the heart-shaped embryos to liquid MS medium without growth regulator. Germination of somatic embryos occurred following the subculture of cotyledonary embryos from liquid MS medium with 0.2 mg/L BAP and 0.02 mg/L naphthalene acetic acid (NAA) to solid hormone-free MS medium. Papaya - Somatic embryogenesis - Case studies 2001-09 Thesis http://psasir.upm.edu.my/id/eprint/10559/ http://psasir.upm.edu.my/id/eprint/10559/1/FP_2001_16.pdf text en public masters Universiti Putra Malaysia Papaya - Somatic embryogenesis - Case studies Faculty of Agriculture Abdul Aziz, Maheran English
institution	Universiti Putra Malaysia
collection	PSAS Institutional Repository
language	English English
advisor	Abdul Aziz, Maheran
topic	Papaya - Somatic embryogenesis - Case studies
spellingShingle	Papaya - Somatic embryogenesis - Case studies Munari, Agus Sutanto Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)
description	This study was carried out with the main objective of establishing somatic embryo production in papaya that may be used for further genetic improvement of the crop. The specific objectives were to induce somatic embryogenesis from immature zygotic embryos, to establish cell suspension culture of somatic embryos and to isolate and culture protoplasts from in vitro leaves. The first experiment studied the effect of growth regulators on the induction and regeneration of somatic embryos from immature zygotic embryos. The combinations of 2,4-dichlorophenoxyacetic acid (2,4-0) and 6-benzylaminopurine (BAP) at various concentrations were assessed in order to determine the best combination for somatic embryogenesis. The experiment was conducted in a Completely Randomized Design. The results showed that higher 2,4-D with or without BAP in the medium increased the percentage of somatic embryo formation and the number of somatic embryos per explant. MS medium supplemented with 5.0 mg/L 2,4-D promoted the highest percentage of somatic embryogenesis (74.55%), while the combinati on of 1.0 mg/L 2,4-0 and 0.01 mg/L BAP produced the highest percentage of callus formation (52. 58%). The highest number of somatic embryos per explant (66.61) was obtained when 5.0 mg/L 2,4-D and 0.01 mg/L BAP were added into MS medium. For germinati on of somatic embryos, the result was very inconsistent; nevertheless, the best treatment for plantlet formation was MS medium without growth hormone (NoBo). The second experiment was the establishment of cell suspensi on culture of somatic embryos. Four weeks after the transfer of somatic embryos into liquid MS medium containing 2.0 mg/L 2,4-0, pro-embryogenic masses (PEMs) were formed in the suspension. Maturation of embryos was achieved on transferring the heart-shaped embryos to liquid MS medium without growth regulator. Germination of somatic embryos occurred following the subculture of cotyledonary embryos from liquid MS medium with 0.2 mg/L BAP and 0.02 mg/L naphthalene acetic acid (NAA) to solid hormone-free MS medium.
format	Thesis
qualification_level	Master's degree
author	Munari, Agus Sutanto
author_facet	Munari, Agus Sutanto
author_sort	Munari, Agus Sutanto
title	Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)
title_short	Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)
title_full	Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)
title_fullStr	Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)
title_full_unstemmed	Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)
title_sort	somatic embryogenesis and protoplast isolation and culture in papaya (carica papaya l.)
granting_institution	Universiti Putra Malaysia
granting_department	Faculty of Agriculture
publishDate	2001
url	http://psasir.upm.edu.my/id/eprint/10559/1/FP_2001_16.pdf
_version_	1804888567583866880

Somatic Embryogenesis and Protoplast Isolation and Culture in Papaya (Carica Papaya L.)

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